Lipoxygenase pathway in model bryophytes: 12-oxo-9(13),15-phytodienoic acid is a predominant oxylipin in Physcomitrella patens

Mukhtarova, Lucia S.; Lantsova, Natalia V.; Хайрутдинов, Б. И.; Grechkin, Alexander N.

doi:10.1016/j.phytochem.2020.112533

Cited by 12 publications

(17 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…dn- iso -OPDA may be synthesized from OPDA by the β-oxidation of iso -OPDA and/or isomerization of dn-OPDA as proposed in M . polymorpha and P. patens ( Monte et al, 2018 ; Mukhtarova et al, 2020 ). In M .…”

Section: Discussionmentioning

confidence: 99%

“…polymorpha , dn- iso -OPDA is most abundant after wounding, followed by OPDA and dn-OPDA, the levels of which are almost comparable ( Monte et al, 2018 ). Additionally, in the model moss P. patens , iso -OPDA is predominant, and smaller amounts of OPDA and dn- iso -OPDA exist with or without the feeding of α-linolenic acid ( Mukhtarova et al, 2020 ). The differences in the profiles of OPDA and its derivatives among the three species indicate that the metabolic rates of isomerization and β-oxidation are different.…”

Section: Discussionmentioning

confidence: 99%

“…dn-OPDA and dn- iso -OPDA are recognized by MpCOI1 to activate downstream signaling ( Monte et al, 2018 ). The model moss Physcomitrium patens , formerly known as Physcomitrella patens , also produces dn-OPDA and dn- iso -OPDA ( Stumpe et al, 2010 ; Mukhtarova et al, 2020 ). Additionally, 12-oxo-9(13),15( Z )-phytodienoic acid ( iso -OPDA; 5 ) has been found in P. patens , but its biological activity has not been revealed ( Mukhtarova et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The model moss Physcomitrium patens , formerly known as Physcomitrella patens , also produces dn-OPDA and dn- iso -OPDA ( Stumpe et al, 2010 ; Mukhtarova et al, 2020 ). Additionally, 12-oxo-9(13),15( Z )-phytodienoic acid ( iso -OPDA; 5 ) has been found in P. patens , but its biological activity has not been revealed ( Mukhtarova et al, 2020 ). Comparative genomics and metabolomics suggest that dn-OPDA and dn- iso -OPDA might act as COI1 ligands in mosses ( Monte et al, 2018 ; Mukhtarova et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, 12-oxo-9(13),15( Z )-phytodienoic acid ( iso -OPDA; 5 ) has been found in P. patens , but its biological activity has not been revealed ( Mukhtarova et al, 2020 ). Comparative genomics and metabolomics suggest that dn-OPDA and dn- iso -OPDA might act as COI1 ligands in mosses ( Monte et al, 2018 ; Mukhtarova et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Deciphering OPDA Signaling Components in the Momilactone-Producing Moss Calohypnum plumiforme

et al. 2021

View full text Add to dashboard Cite

Jasmonic acid (JA) and its biologically active form jasmonoyl-L-isoleucine (JA-Ile) regulate defense responses to various environmental stresses and developmental processes in plants. JA and JA-Ile are synthesized from α-linolenic acids derived from membrane lipids via 12-oxo-phytodienoic acid (OPDA). In the presence of JA-Ile, the COI1 receptor physically interacts with JAZ repressors, leading to their degradation, resulting in the transcription of JA-responsive genes by MYC transcription factors. Although the biosynthesis of JA-Ile is conserved in vascular plants, it is not recognized by COI1 in bryophytes and is not biologically active. In the liverwort Marchantia polymorpha, dinor-OPDA (dn-OPDA), a homolog of OPDA with two fewer carbons, and its isomer dn-iso-OPDA accumulate after wounding and are recognized by COI1 to activate downstream signaling. The moss Calohypnum plumiforme produces the antimicrobial-specialized metabolites, momilactones. It has been reported that JA and JA-Ile are not detected in C. plumiforme and that OPDA, but not JA, can induce momilactone accumulation and the expression of these biosynthetic genes, suggesting that OPDA or its derivative is a biologically active molecule in C. plumiforme that induces chemical defense. In the present study, we investigated the biological functions of OPDA and its derivatives in C. plumiforme. Searching for the components potentially involving oxylipin signaling from transcriptomic and genomic data revealed that two COI1, three JAZ, and two MYC genes were present. Quantification analyses revealed that OPDA and its isomer iso-OPDA accumulated in larger amounts than dn-OPDA and dn-iso-OPDA after wounding. Moreover, exogenously applied OPDA, dn-OPDA, or dn-iso-OPDA induced the transcription of JAZ genes. These results imply that OPDA, dn-OPDA, and/or their isomers potentially act as biologically active molecules to induce the signaling downstream of COI1-JAZ. Furthermore, co-immunoprecipitation analysis showed the physical interaction between JAZs and MYCs, indicating the functional conservation of JAZs in C. plumiforme with other plants. These results suggest that COI1-JAZ-MYC mediated signaling is conserved and functional in C. plumiforme.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%