Lipoxygenase Inhibitors Nordihydroguaiaretic Acid and Fungus Lecanicillum lecanii Extract Induce Death of Lymphoid Leukemia Cells

Bibikova, Marina; Spiridonova, N. A.; Korystova, A. F.; Kublik, L. N.; Levitman, M. Kh.; Shaposhnikova, V. V.; Korystov, Yu. N.

doi:10.1007/s10517-017-3796-9

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…It is widely distributed in the warm deserts of North American and Argentina [2], and known to attain ages of several thousand years [3]. Apart from the pharmacological use of L. tridentata in traditional system of medicine for diverse ailments [4], it contains the potent antioxidant phenolic lignan / phenylpropanoids, nordihydroguaiaretic acid (NDGA) [4][5][6][7][8][9] which exerts in vitro anti-cancer effects [10,11].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome characterization of Larrea tridentata and identification of genes associated with phenylpropanoid metabolic pathways

et al. 2022

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Larrea tridentata (Sesse and Moc. ex DC.) Coville (family: Zygophyllaceae) is an aromatic evergreen shrub with resin-covered leaves, known to use in traditional medicine for diverse ailments. It also has immense pharmacological significance due to presence of powerful phenylpropanoids antioxidant, nordihydroguaiaretic acid (NDGA). The RNA sequence/transcriptome analyses connect the genomic information into the discovery of gene function. Hence, the acquaint analysis of L. tridentata is in lieu to characterize the transcriptome, and to identify the candidate genes involved in the phenylpropanoid biosynthetic pathway. To gain molecular insight, the bioinformatics analysis of transcriptome was performed. The total bases covered 48,630 contigs of length greater than 200 bp and above came out to 21,590,549 with an average GC content of 45% and an abundance of mononucleotide, SSR, including C3H, FAR1, and MADS transcription gene families. The best enzyme commission (EC) classification obtained from the assembled sequences represented major abundant enzyme classes e.g., RING-type E3 ubiquitin transferase and non-specific serine/threonine protein kinase. The KEGG pathway analysis mapped into 377 KEGG different metabolic pathways. The enrichment of phenylpropanoid biosynthesis pathways (22 genes i.e., phenylalanine ammonia-lyase, trans-cinnamate 4-monooxygenase, 4-coumarate-CoA ligase, cinnamoyl-CoA reductase, beta-glucosidase, shikimate O-hydroxycinnamoyl transferase, 5-O-(4-coumaroyl)-D-quinate 3’-monooxygenase, cinnamyl-alcohol dehydrogenase, peroxidase, coniferyl-alcohol glucosyltransferase, caffeoyl shikimate esterase, caffeoyl-CoA O-methyltransferase, caffeate O-methyltransferase, coniferyl-aldehyde dehydrogenase, feruloyl-CoA 6-hydroxylase, and ferulate-5-hydroxylase), and expression profile indicated antioxidant, anti-arthritic, and anticancer properties of L. tridentata. The present results could provide an important resource for squeezing biotechnological applications of L. tridentata.

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Section: Introductionmentioning

confidence: 99%

Transcriptome characterization of Larrea tridentata and identification of genes associated with phenylpropanoid metabolic pathways

et al. 2022

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“…The nordihydroguaiaretic acid (NDGA) is the most abundant and studied metabolite of LT [ 22 ]. NDGA is a potent inhibitor of lipoxygenase and cyclooxygenase pathway [ 26 ]. Tea brewed of LT was used to treat digestive disorders, rheumatism and most common colds [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Tea brewed of LT was used to treat digestive disorders, rheumatism and most common colds [ 23 ]. In 2018, Chan et al reported that NDGA protect against obesity in vivo model [ 26 ]. LT is a rich source of metabolites including phenolic, terpenoids, saponins, and odorous vinyl ketones ( Figure 1 ) [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Assessing the Antioxidant Properties of Larrea tridentata Extract as a Potential Molecular Therapy against Oxidative Stress

et al. 2018

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Oxidative stress has been linked to neurodegenerative diseases such as Huntington’s, Parkinson’s, Alzheimer’s and amyotrophic lateral sclerosis diseases. Larrea tridentata (LT) also known as Creosote Bush is an evergreen shrub found in the Chihuahuan desert which has been used medicinally by Native American tribes in southwestern North America and the Amerindians of South America. However, studies of the antioxidant capacity of the crude extract of LT towards the discovery of novel molecular therapies bearing antioxidants and drug-like properties are lacking. In this study, we assessed the antioxidant properties of Larrea tridentata, collected specifically from the Chihuahuan desert in the region of El Paso del Norte, TX, USA. LT phytochemicals were obtained from three different extracts (ethanol; ethanol: water (60:40) and water). Then the extracts were evaluated in eight different assays (DPPH, ABTS, superoxide; FRAP activity, nitric oxide, phenolic content, UV visible absorption and cytotoxicity in non-cancerous HS27 cells). The three extracts were not affecting the HS27 cells at concentrations up to 120 µg/mL. Among the three extracts, we found that the mixture of ethanol: water (60:40) LT extract has the most efficient antioxidant properties (IC50 (DPPH at 30 min) = 111.7 ± 3.8 μg/mL; IC50 (ABTS) = 8.49 ± 2.28 μg/mL; IC50 (superoxide) = 0.43 ± 0.17 μg/mL; IC50 (NO) = 230.4 ± 130.4 μg/mL; and the highest phenolic content was estimated to 212.46 ± 7.05 mg GAE/L). In addition, there was a strong correlation between phenolic content and the free-radical scavenging activity assays. HPLC-MS study identified nine compounds from the LT-ethanol: water extract including Justicidin B and Beta peltain have been previously reported as secondary metabolites of Larrea tridentata.

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“…Disruption of the actin cytoskeleton in association with the activation of stress-activated protein kinases has also been observed (Seufferlein et al, 2002). NDGA, along with the extract from the fungus Lecanicillum lecanii, inhibits the growth of lymphatic leukemia cells by inducing nuclear damage leading to reduced DNA content in the cells (Bibikova et al, 2017). Besides, the compound causes cell death in AML cell lines as well as in patient samples via inhibition of AKT phosphorylation, suggesting that the extrinsic and the mitochondrial apoptotic pathways are not essential for cell death (Mak et al, 2007).…”

Section: Discussionmentioning

confidence: 97%

Repurposing Drugs by In Silico Methods to Target BCR Kinase Domain in Chronic Myeloid Leukemia

Natarajan¹,

Rajkumar²,

Sabitha³

2019

Asian Pac J Cancer Prev

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Lipoxygenase Inhibitors Nordihydroguaiaretic Acid and Fungus Lecanicillum lecanii Extract Induce Death of Lymphoid Leukemia Cells

Cited by 5 publications

References 12 publications

Transcriptome characterization of Larrea tridentata and identification of genes associated with phenylpropanoid metabolic pathways

Transcriptome characterization of Larrea tridentata and identification of genes associated with phenylpropanoid metabolic pathways

Assessing the Antioxidant Properties of Larrea tridentata Extract as a Potential Molecular Therapy against Oxidative Stress

Repurposing Drugs by In Silico Methods to Target BCR Kinase Domain in Chronic Myeloid Leukemia

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