Lipotoxic Effect of p21 on Free Fatty Acid-Induced Steatosis in L02 Cells

Wang, Jie-wei; Wan, Xingyong; Zhu, Hongyan; Lü, Chao; Yu, Wei-Lai; Shen, Zhe; Li, Youming

doi:10.1371/journal.pone.0096124

Cited by 12 publications

(11 citation statements)

References 33 publications

(45 reference statements)

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“…It seems that 10 mg/dl UA at 96 h has a beneficial effect. However, for AST, we could not found a tendency dependent on time, This result conflicts with that of Wang et al (17), and it needs to be further researched. ALT is the enzyme that catalyzes the conversions of α-ketoglutarate to glutamate and pyruvic acid to alanine and is a rate-controlling enzyme for gluconeogenesis in the liver (34).…”

Section: Discussioncontrasting

confidence: 99%

“…The method for dissolving oleic acid (OA) and developing a steatosis cell model was similar to that previously published by Wang et al (17). Briefly, OA (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and NaOH were mixed in ultrapure water, and the solution was added to the standard medium to prepare a sodium oleate medium with OA concentrations of 0.1, 0.2, 0.3, and 0.4 mM.…”

Section: Methodsmentioning

confidence: 99%

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Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Cheng

Yang²,

Zhou

et al. 2018

Exp Ther Med

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Abstract. The development of nonalcoholic fatty liver disease (NAFLD) is caused by the steatosis of hepatocytes, which induces oxidative stress (OS). Thus, OS has an important role in the development of NAFLD. In the present study, the L-02 hepatocyte cell line was used to develop a steatosis cell model. The best model was determined using an MTT assay and the triglyceride levels. Model cells were treated with high concentrations of uric acid (UA; 0, 5, 10, 20 and 30 mg/dl) for 24, 48, 72 and 96 h. Indicators of oxidation were then measured, which included total superoxide dismutase (SOD), malonaldehyde (MDA) and reduced glutathione (GSH), and the transcriptional and translational levels of SOD1 and γ-glutamate-cysteine ligase (γ-GCLC) were also determined. In addition, the intracellular levels of aspartate aminotransferase and alanine aminotransferase (ALT) were detected. The activity of SOD1 decreased over time and the result was supported by the results of western blotting. The transcriptional levels of SOD1 in model cells was significantly higher than untreated cells at 48 h. With the decreased levels of SOD1 and GSH, MDA increased in a time-dependent manner. The content of GSH decreased with time as well, which was also reflected in the results of western blotting. The transcriptional levels of γ-GCLC in all UA-treated groups were lower when compared with those observed in the model group. The activity of ALT tended to increase, depending on the duration of treatment. Treatment with 5 and 10 mg/dl UA had an antioxidative effect on the model cells, and 30 mg/dl UA treatment for 48 h increased OS in the cells.

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Section: Discussioncontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Cheng

Yang²,

Zhou

et al. 2018

Exp Ther Med

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“…The offspring that received high saturated fat input during perinatal life could have adapted to down-regulate the lipid catabolism machinery, reducing fat utilization as energy source. The development of this adaptation might be the cause of liver lipid overaccumulation that induces lipotoxicity and is harmful for liver function [46,47]. PPARα expression showed no changes in all the developmental stages studied.…”

Section: Discussionmentioning

confidence: 99%

Maternal saturated-fat-rich diet promotes leptin resistance in fetal liver lipid catabolism and programs lipid homeostasis impairments in the liver of rat offspring

Mazzucco

Fornes

Capobianco

et al. 2016

The Journal of Nutritional Biochemistry

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“…Nedavna definicija ukazuje da je stres stanje u kojem pritisak na homeostazu nadilazi regulatorne kapacitete za očuvanje homeostaze, tj. zahtjevi (mikro)okoliša su veći od kapaciteta regulatornih reakcija 87 . Klasifikacija stresa je etiopatogenetska.…”

Section: Ahilova Peta Stanice -Definicija Jezgrice I Bolesti U 21 Stunclassified

Descriptive and functional definition of nucleolus – a pathway to cancer therapy

et al. 2016

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Sažetak. Očekuje se da moderna terapija tumora djeluje ciljano i sveobuhvatno. U novim kliničkim ispitivanjima terapije raka primjenjuju se mali molekularni inhibitori transkripcije ribosomalnih gena i aktivatori apoptoze. Model terapije prikazan ovdje je aktivacija supresora tumora koji djeluju u domeni jezgrice. Stanice tumora pokazuju poremećaj regulacije proteina i mutaciju P53 gena koji suprimira rast. Razvoj bolesti definira terapiju. Moderna se definicija bolesti temelji na etiopatogenezi staničnog stresa u kojem sudjeluju brojne organele, uključujući jezgricu. Jezgra i jezgrica su mikroskopski oblici. Ovaj prikaz daje sažet opis onoga što se vidi kada se kaže "jezgrica". Nadalje su opisani kiseli nehistonski proteini koji se okupljaju oko transkripcijskog ustroja RNK polimeraze I te je istaknut značaj njihove vizualizacije za promjenu definicije jezgrice. Osim što je tjelešce u nukleoplazmi i subnuklearna organela, jezgrica se može vizualizirati pomoću njezinih funkcionalnih struktura -AgNOR-a. AgNOR proteini imaju ulogu u nukleolarnom stresu. Svrha ovoga teksta je raspraviti nukleolarni stres.Ključne riječi: biološki stres; bolest; karcinogeneza; stanična jezgrica; terapija Abstract. Modern tumour therapy should be target specific and comprehensive. Recent clinical trials test small molecular inhibitors of transcription of ribosomal genes and activators of apoptosis. We treatise on models of activation of the suppressors of tumour growth acting in the nucleolar domain. Tumour cells demonstrate a disordered protein regulation and mutation of P53 gene which codes for a suppressor of growth. Therapy is guided by pathogenesis. Modern disease definition is based on etiopathogenesis of cell stress, wherein participate numerous organelles, including the nucleolus. Nucleus and nucleolus are forms seen on light microscopy. This presentation focuses on the description of the visible correlate of the notion of "nucleolus". Further to this, acid non-histone proteins which gather around the transcriptional machinery of RNA polymerase I are described, and the significance of their visualisation towards the alteration of the definition of nucleolus is pointed out. Besides being a particle within the nucleoplasm and a subnuclear organelle, the nucleolus is visualized through its functional structures -the AgNORs. The AgNOR proteins have a role in nucleolar stress. The aim of this article is to discuss the nucleolar stress.

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Lipotoxic Effect of p21 on Free Fatty Acid-Induced Steatosis in L02 Cells

Cited by 12 publications

References 33 publications

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Maternal saturated-fat-rich diet promotes leptin resistance in fetal liver lipid catabolism and programs lipid homeostasis impairments in the liver of rat offspring

Descriptive and functional definition of nucleolus – a pathway to cancer therapy

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