Lipoteichoic acid fromLactobacillus plantarumelicits both the production of Interleukin-23p19 and suppression of pathogen-mediated Interleukin-10 in THP-1 cells

Abstract

In this study, the stimulatory effects of different lactic acid bacteria strains, and their subcellular fractions, on the THP-1 cell line were evaluated. Lactobacillus plantarum was found in particular to induce high levels of IL-23p19 mRNA, but it moderately induced TNF-a production. IL-10 production was not entirely affected by L. plantarum stimulation. When subcellular fractions of L. plantarum were used to treat THP-1 cells, IL-23p19 mRNA expression was enhanced in a doseresponsive manner, specifically by lipoteichoic acid (LTA). The cotreatment of THP-1 cells by both L. plantarum and Staphylococcus aureus LTA resulted in decreased IL-10 production when compared with cells treated by S. aureus LTA alone. Taken together, these data suggest that LTA isolated from L. plantarum elicits stimulatory effects upon the expression of IL-23p19 and inhibitory effects on pathogen-mediated IL-10 production.

Piliation of Lactobacillus rhamnosus GG Promotes Adhesion, Phagocytosis, and Cytokine Modulation in Macrophages

“…Furthermore, teichoic acids (TAs) from L. plantarum ATCC 14917 were found to be recognized by TLR2, activate the extracellular signal-regulated kinase pathway, and act synergistically with L. casei Shirota YIT9029 to induce IL-10 production in mouse peritoneal macrophages (55). However, other studies showed that expression of IL-10 was almost entirely unaffected in THP-1 cells after treatment with LTA isolated from L. plantarum KCTC10887BP (56) and that LTA from L. plantarum NCIMB8826 promotes IL-12 rather than IL-10 induction (57). Clearly, multiple Lactobacillus cell wall or secreted molecules could be directly involved in IL-10 mRNA induction.…”

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

“…Whole cell bacteria and subcellular bacterial fractions were prepared from S. flexneri (KCTC 2517), L. plantarum K8 (KCTC10887BP), and S. aureus (KCTC 1621) as previously described (Kim et al, 2007a). P. gingivalis LPS (PGLPS), muramyl dipeptide (MDP), Pam3CSK4, and monophosphoryl lipid A from S. minnesota (MPLA) were purchased from Invivogen (USA).…”

Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Lipopolysaccharide-Induced TNF-α Production in THP-1 Cells and Endotoxin Shock in Mice

“…The purity of the purified LTA was determined by measuring the protein and endotoxin contents through the conventional silver staining after PAGE and through the Limulus amebocyte lysate assay (BioWhittaker), respectively. DNA or RNA contamination was assessed by measuring UV absorption at 260 and 280 nm (12). The modification of LTA from L. plantarum was performed as previously described with minor modifications (5, 13).…”