Liposomes with Caffeic Acid: Morphological and Structural Characterisation, Their Properties and Stability in Time

Dejeu, Ioana Lavinia; Vicaş, Laura; Jurca, Tünde; Teușdea, Alin Cristian; Mureşan, Mariana; Fritea, Luminița; Svera, Paula; Gabor, Gianina; Dejeu, George Emanuiel; Maghiar, Octavian; Bodea, Anca Salomea; Pallag, Annamária; Marian, Eleonora

doi:10.3390/pr9060912

Cited by 9 publications

(6 citation statements)

References 43 publications

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“…Moreover, it was observed that the release of entrapped CA occurs gradually. In the first eight hours the release is at its high (over 80%), after which it is getting reduced 50 . Katuwavila et al 51 prepared caffeic acid-loaded egg phosphatidylcholine liposomes with a similar encapsulation efficiency of nearly 70%.…”

Section: Discussionmentioning

confidence: 99%

“…According to Caddeo et al 49 , in general, liposomal encapsulation is not significantly influenced by the lipids used for the preparation of the liposomes. Dejeu et al 50 determining of entrapment efficiency showed that, regardless of the composition of phospholipids (PC, DMPC and DPPC), the percentage of CA entrapment was up to 76%. Moreover, it was observed that the release of entrapped CA occurs gradually.…”

Section: Discussionmentioning

confidence: 99%

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The influence of the pH on the incorporation of caffeic acid into biomimetic membranes and cancer cells

Naumowicz

Kusaczuk

Zając

et al. 2022

Sci Rep

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Caffeic acid (CA) is a phenolic compound synthesized by all plant species. It constitutes the main hydroxycinnamic acid found in human diet and presents a variety of beneficial effects including anticancer activity. Current data suggests essential role of the interplay between anticancer drugs and the cell membrane. Given this, biophysical interactions between CA and cancer cells or biomimetic membranes were investigated. Glioblastoma cell line U118MG and colorectal adenocarcinoma cell line DLD-1, as well as lipid bilayers and liposomes, were used as in vitro models. Electrophoretic light scattering was used to assess the effect of CA on the surface charge of cancer cells and liposomal membranes. Electrochemical impedance spectroscopy was chosen to evaluate CA-dependent modulatory effect on the electrical capacitance and electrical resistance of the bilayers. Our results suggest that CA fulfills physicochemical criteria determining drug-like properties of chemical compounds, and may serve as a potential cytostatic agent in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The influence of the pH on the incorporation of caffeic acid into biomimetic membranes and cancer cells

Naumowicz

Kusaczuk

Zając

et al. 2022

Sci Rep

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“…In vitro release studies were conducted using the cellulose dialysis membrane diffusion technique, according to Dejeu’s work [ 23 ]. Prior to beginning the assay, cellulose dialysis membranes (Float-A-Lyzer G2, CE, 10 kDa, SpectrumLabs, Los Angeles, CA, USA) were equilibrated at 4 °C with ultrapure water for 12 h, which was replaced with PBS for 1 h. The donor chamber was filled with 1 mL of GA-loaded Tf-functionalized liposomes.…”

Section: Methodsmentioning

confidence: 99%

Transferrin-Functionalized Liposomes for the Delivery of Gallic Acid: A Therapeutic Approach for Alzheimer’s Disease

2022

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Senile plaques composed of amyloid β (Aβ) fibrils are considered the leading cause of Alzheimer’s disease (AD). Molecules with the ability to inhibit Aβ aggregation and/or promote Aβ clearance are thus a promising approach for AD therapy. Our group recently demonstrated that gallic acid (GA) has strong anti-amyloidogenic properties. In this study, stealth liposomes were prepared for the delivery of GA for AD therapy. The liposomes were functionalized with transferrin (Tf) to direct them to the brain, since Tf receptors are overexpressed in the endothelial cells of the blood–brain barrier. GA-loaded Tf-functionalized liposomes showed mean diameters of 130 nm, low polydispersity index values, and neutral zeta potential. Moreover, the produced nanocarriers promoted the sustained release of GA over 5 days and are physically stable for 1 month under storage conditions. Furthermore, GA-loaded Tf-functionalized liposomes showed a strong ability to interact with Aβ1-42 monomers, slowing down the Aβ monomer-to-oligomer and oligomer-to-fibril transitions and decreasing the number of fibrils formed by 56%. In addition, the NPs disaggregated approximately 30% of preformed Aβ fibrils. The presented results suggest that Tf-functionalized liposomes could be a viable platform for the brain delivery of GA for AD therapy. Studies with animal models of AD will be valuable for validating the therapeutic efficacy of this novel liposomal formulation.

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“…The characteristics of liposomes containing CAPE, including size, zeta potential, PDI, and EE%, as well as their morphology, were determined. The results obtained from the EE% indicated that the liposomes were able to load a significant amount of CAPE, as per Dejeu et al37 This study employed HSPC, cholesterol, and DSPE-PEG2000 for the synthesis of liposomes. The efficiency of the liposome was found to increaseF I G U R E 5 The release percentage of (A) liposomal caffeic acid phenethyl ester (CAPE) and (B) free CAPE versus time at different pH values.…”

mentioning

confidence: 52%

The anticancer impacts of free and liposomal caffeic acid phenethyl ester (CAPE) on melanoma cell line (A375)

Bahrami,

Farasat,

Zolghadr

et al. 2023

Cell Biochemistry & Function

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The deadliest type of skin cancer, malignant melanoma, is also the reason for the majority of skin cancer‐related deaths. The objective of this article was to investigate the efficiency of free caffeic acid phenethyl ester (CAPE) and liposomal CAPE in inducing apoptosis in melanoma cells (A375) in in vitro. CAPE was loaded into liposomes made up of hydrogenated soybean phosphatidylcholine, cholesterol, and 1,2‐distearoyl‐sn‐glycero‐3 phosphoethanolamine‐N‐[methoxy (polyethylene glycol)‐2000], and their physicochemical properties were assessed. (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) test was performed for comparing the cytotoxicity of free CAPE and liposomal CAPE at dosages of 10, 15, 25, 50, 75 and the highest dose of 100 μg/mL for period of 24 and 48 h on A375 cell line to calculate IC50. Apoptosis and necrosis were evaluated in A375 melanoma cancer cells using flow cytometry. Atomic force microscopy was utilized to determine the nanomechanical attributes of the membrane structure of A375 cells. To determine whether there were any effects on apoptosis, the expression of PI3K/AKT1 and BAX/BCL2 genes was analyzed using the real‐time polymerase chain reaction technique. According to our results, the maximum amount of drug release from nanoliposomes was determined to be 91% and the encapsulation efficiency of CAPE in liposomes was 85.24%. Also, the release of free CAPE was assessed to be 97%. Compared with liposomal CAPE, free CAPE showed a greater effect on reducing the cancer cell survival after 24 and 48 h. Therefore, IC50 values of A375 cells treated with free and liposomal CAPE were calculated as 47.34 and 63.39 μg/mL for 24 h. After 48 h of incubation of A375 cells with free and liposomal CAPE, IC50 values were determined as 30.55 and 44.83 μg/mL, respectively. The flow cytometry analysis revealed that the apoptosis induced in A375 cancer cells was greater when treated with free CAPE than when treated with liposomal CAPE. The highest nanomechanical changes in the amount of cell adhesion forces, and elastic modulus value were seen in free CAPE. Subsequently, the greatest decrease in PI3K/AKT1 gene expression ratio occurred in free CAPE.

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Liposomes with Caffeic Acid: Morphological and Structural Characterisation, Their Properties and Stability in Time

Cited by 9 publications

References 43 publications

The influence of the pH on the incorporation of caffeic acid into biomimetic membranes and cancer cells

The influence of the pH on the incorporation of caffeic acid into biomimetic membranes and cancer cells

Transferrin-Functionalized Liposomes for the Delivery of Gallic Acid: A Therapeutic Approach for Alzheimer’s Disease

The anticancer impacts of free and liposomal caffeic acid phenethyl ester (CAPE) on melanoma cell line (A375)

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