Liposomes: Versatile and Biocompatible Nanovesicles for Efficient Biomolecules Delivery

Mallick, Sudipta; Choi, Joon Sig

doi:10.1166/jnn.2014.9080

Cited by 133 publications

(97 citation statements)

References 73 publications

(81 reference statements)

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“…TTX is very hydrophilic and therefore readily encapsulated within liposomes, like some other site 1 sodium-channel blockers (6,7). Liposomes were selected as the drug-delivery vehicle because of their biocompatibility, injectability, acceptable drug-loading levels, and track record of clinical use and safety (37,38). To enhance the encapsulation of TTX, which contains a positively charged guanidinium group (39), a negatively charged lipid, 1,2-distearoyl-sn-glycero-3-phosphatidylglycerol (DSPG), was added in the liposome formulation, as we have done in encapsulating other cationic site 1 sodiumchannel blockers (6,7).…”

Section: Significancementioning

confidence: 99%

Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes

Rwei

Lee

Zhan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

105

127

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Pain management would be greatly enhanced by a formulation that would provide local anesthesia at the time desired by patients and with the desired intensity and duration. To this end, we have developed near-infrared (NIR) light-triggered liposomes to provide on-demand adjustable local anesthesia. The liposomes contained tetrodotoxin (TTX), which has ultrapotent local anesthetic properties. They were made photo-labile by encapsulation of a NIR-triggerable photosensitizer; irradiation at 730 nm led to peroxidation of liposomal lipids, allowing drug release. In vitro, 5.6% of TTX was released upon NIR irradiation, which could be repeated a second time. The formulations were not cytotoxic in cell culture. In vivo, injection of liposomes containing TTX and the photosensitizer caused an initial nerve block lasting 13.5 ± 3.1 h. Additional periods of nerve block could be induced by irradiation at 730 nm. The timing, intensity, and duration of nerve blockade could be controlled by adjusting the timing, irradiance, and duration of irradiation. Tissue reaction to this formulation and the associated irradiation was benign.pain | site 1 sodium-channel blocker | photosensitizer | light | near infrared

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Section: Significancementioning

confidence: 99%

Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes

Rwei

Lee

Zhan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

105

127

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“…3,4 Liposomes are the best-known example of artificial nanosized vesicles, and consist of a phospholipid bilayer entrapping an aqueous core. 5,6 They are successfully used for a number of biomedical purposes: from model systems to study biological membranes to efficient vectoring carriers for delivering bioactive substances or other molecules to pathological sites. [7][8][9] The need for systematic approaches to optimize specific membrane physico-chemical characteristics (e.g.…”

Section: Introductionmentioning

confidence: 99%

Novel stable dendrimersome formulation for safe bioimaging applications

et al. 2015

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a Dendrimersomes are nanosized vesicles constituted by amphiphilic Janus dendrimers (JDs), which have been recently proposed as innovative nanocarriers for biomedical applications. Recently, we have demonstrated that dendrimersomes self-assembled from (3,5)12G1-PE-BMPA-G2-(OH) 8 dendrimers can be successfully loaded with hydrophilic and amphiphilic imaging contrast agents. Here, we present two newly synthesized low generation isomeric JDs: JDG0G1(3,5) and JDG0G1(3,4). Though less branched than the above-cited dendrimers, they retain the ability to form self-assembled, almost monodisperse vesicular nanoparticles. This contribution reports on the characterization of such nanovesicles loaded with the clinically approved MRI probe Gadoteridol and the comparison with the related nanoparticles assembled from more branched dendrimers. Special emphasis was given to the in vitro stability test of the systems in biologically relevant media, complemented by preliminary in vivo data about blood circulation lifetime collected from healthy mice. The results point to very promising safety and stability profiles of the nanovesicles, in particular for those made of JDG0G1(3,5), whose spontaneous self-organization in water gives rise to a homogeneous suspension. Importantly, the blood lifetimes of these systems are comparable to those of standard liposomes. By virtue of the reported results, the herein presented nanovesicles augur well for future use in a variety of biomedical applications.

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“…Phospholipids are cell membrane component, so liposome made from lipid mixture (CHEMS/NHS/TEA/PEG) is wellknown non-cytotoxicity [25]. As show in figure 5, there was no significant difference in cell viability over the liposome concentration range of 0 to 4 mg ml -1 for 24 h or 48 h in MCF-7 cells ( figure 5(a)) and HCT116 cells ( figure 5(b)).…”

Section: Cytotoxicity Study By Mtt Methodsmentioning

confidence: 95%

Enhanced anticancer efficacy and tumor targeting through folate-PEG modified nanoliposome loaded with 5-fluorouracil

Tran

et al. 2017

Adv. Nat. Sci: Nanosci. Nanotechnol.

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Cancer targeted therapies have attracted considerable attention over the past year. Recently, 5-fluouracil (5-FU), which has high toxicity to normal cells and short half-life associated with rapid metabolism, is one of the most commonly used therapies in the treatment of cancer. In this study the folic acid-conjugated pegylated nanoliposomes were synthesized and then loaded into them with 5-FU to improve the anti-tumor efficacy. The average size of liposomes (LPs) was about 52.7 nm which was identified by TEM. In the liposome uptake studies, the level uptake of folate-conjugated liposomes has increased compared to non-conjugated LPs according to LPs concentration, incubation time and presence of concentration of free folic acid (FA). The MTT assay and apoptotic test were carried out in HCT116 and MCF-7 cells for 24 or 48 h. The results revealed that the folate-PEG modified 5-Fu loaded nanoliposomes had strong cytotoxicity to cancer cell compared to pure 5-FU or PEG modified 5-FU loaded liposomes in a concentration- and time-dependent manner, and mainly enhanced the cancer cell death through folate-mediated endocytosis. Hence, the folate-PEG modified nanoliposome is a potential targeted drug-delivery system for the treatment of FR-positive cancers.

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Liposomes: Versatile and Biocompatible Nanovesicles for Efficient Biomolecules Delivery

Cited by 133 publications

References 73 publications

Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes

Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes

Novel stable dendrimersome formulation for safe bioimaging applications

Enhanced anticancer efficacy and tumor targeting through folate-PEG modified nanoliposome loaded with 5-fluorouracil

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