Liposomes: vehicles for the targeted and controlled delivery of peptides and proteins

Crommelin, Daan J.A.; Daemen, Toos; Scherphof, Gerrit L.; Vingerhoeds, Monique H.; Heeremans, J.L.M.; Kluft, C.; Storm, Gert

doi:10.1016/s0168-3659(96)01583-0

Cited by 29 publications

(14 citation statements)

References 45 publications

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“…PEGylation is generally described as the molecular attachment of polyethylene glycols (PEGs) with different molecular weights to active drug molecules or surface treatment of drug-bearing particles with PEGs and is one of the most promising and extensively studied strategies for improving the pharmacokinetic behavior of therapeutic drugs by increasing their circulation time in the body. In this article, a simple thin film hydration method was used for preparing conventional and PEGylated liposomes (Crommelin et al, 1997) through a combination of high pressure homogenizer and extrusion through polycarbonate membrane filters to form uniform size vesicles (monodisperse) for intravenous administration to improve their pharmacokinetic profiles, conventional and PEGylated liposome's by prolonging their circulation time in the blood. Further optimized formulations were subjected to Fourier transform infrared (FTIR) study, differential scanning calorimetry (DSC) analysis, particle size distribution, zeta potential, in vitro cytotoxicity, stability studies and in vivo behavior in rats.…”

Section: Introductionmentioning

confidence: 99%

PEGylated liposomes of anastrozole for long-term treatment of breast cancer:in vitroandin vivoevaluation

Shavi

Reddy

Raghavendra

et al. 2015

Journal of Liposome Research

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The aim of present study was to develop conventional and PEGylated (long circulating), liposomes containing anastrozole (ANS) for effective treatment of breast cancer. ANS is a third-generation non-steroidal aromatase inhibitor of the triazole class used for the treatment of advanced and late-stage breast cancer in post-menopausal women. Under such disease conditions the median duration of therapy should be prolonged until tumor regression ends (>31 months). Liposomes were prepared by the thin film hydration method by using ANS and various lipids such as soyaphosphatidyl choline, cholesterol and methoxy polyethylene glycol distearoyl ethanolamine in different concentration ratios and evaluated for physical characteristics, in vitro drug release and stability. Optimized formulations of liposome were studied for in vitro cytotoxic activity against the BT-549 and MCF-7 cell lines and in vivo behavior in Wistar rats. Preformulation studies, both Fourier transform infrared study and differential scanning calorimetry analysis showed no interaction between the drug and the excipients used in the formulations. The optimized formulations AL-07 and AL-09 liposomes showed encapsulation efficiencies in the range 65.12 ± 1.05% to 69.85 ± 3.2% with desired mean particle size distribution of 101.1 ± 5.9 and 120.2 ± 2.8 nm and zeta potentials of -43.7 ± 4.7 and -62.9 ± 3.5 mV. All the optimized formulations followed Higuchi-matrix release kinetics and when plotted in accordance with the Korsemeyer-Peppas method, the n-value 0.5 < n < 1.0 suggests an anomalous (non-Fickian) transport. Likewise, the PEGylated liposomes showed greater tumor growth inhibition on BT-549 and MCF-7 cell lines from in vitro cytotoxicity studies (p < 0.05). Pharmacokinetic study of conventional and PEGylated liposomes in Wistar rats demonstrated a 3.33- and 20.28-fold increase in AUC(0-∞) values when compared to pure drug (p < 0.001). Among the formulations, PEGylated liposomes showed encouraging results by way of their long circulation and sustained delivery properties for effective treatment of breast cancer.

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Section: Introductionmentioning

confidence: 99%

PEGylated liposomes of anastrozole for long-term treatment of breast cancer:in vitroandin vivoevaluation

Shavi

Reddy

Raghavendra

et al. 2015

Journal of Liposome Research

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“…One of the most useful is that based on ligand/receptor recognition (4)(5)(6). This alternative requires the identification of some biological structures selectively expressed in the membrane of tumoral cells as, for instance, laminin whose receptors (integrins) are overexpressed in proliferant cells (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic Laminin-Related Peptides: Synthesis and Surface Properties

Reig

Haro

Polo

et al. 2001

Journal of Colloid and Interface Science

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“…Selective combination of the lipid components used in liposome formulation can achieve controlled stability of the liposomal membrane and selective release of encapsulated substances under specific environmental conditions [79]. One approach involves the use of pH-sensitive lipids such as phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (POPE).…”

Section: Controlled and Enhanced Processingmentioning

confidence: 99%

Particulate Systems as Adjuvants and Carriers for Peptide and Protein Antigens

Liang¹,

Davies²,

Blanchfield³

et al. 2006

CDD

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The most common feature for antigen-delivery systems is their particulate nature. Together with a certain depot effect, it is the particulate nature that primarily dictates whether the antigen-delivery system will be successful in inducing a certain type and strength of immune response. In this article, we will summarize recent data on particulate delivery systems for peptide and protein antigens with a main focus on lipid or polymer-based particles, all of which possess high potential as both preventive and therapeutic vaccines for parenteral, nasal, and possibly oral administration.

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Liposomes: vehicles for the targeted and controlled delivery of peptides and proteins

Cited by 29 publications

References 45 publications

PEGylated liposomes of anastrozole for long-term treatment of breast cancer:in vitroandin vivoevaluation

PEGylated liposomes of anastrozole for long-term treatment of breast cancer:in vitroandin vivoevaluation

Hydrophobic Laminin-Related Peptides: Synthesis and Surface Properties

Particulate Systems as Adjuvants and Carriers for Peptide and Protein Antigens

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