Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells

Jiang, Li; Ayre, Wayne Nishio; Melling, Genevieve E; Song, Bing; Wei, Xiao-Qing; Sloan, Alastair James; Chen, Xu

doi:10.1016/j.jdent.2020.103501

Cited by 16 publications

(7 citation statements)

References 34 publications

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“…These systems have demonstrated improved odontogenic differentiation, suggested by the upregulated levels of osteodentin, RUNX-2, DMP-1, and DSPP markers in human dental pulp stem cells (hDPSCs) in vitro. [266][267][268] Dental restorations are often linked with cytotoxic and inflammatory response due to the localized accumulation of unreacted monomer that leaches out in the surrounding environment. 269,270 This is further aggravated by bacterial and enzyme-mediated polymer composite hydrolysis, [271][272][273][274] which thereby influence the activity and biochemistry of the oral microbiome.…”

Section: Dental Pulp Dentin and Alveolar Bone Regenerationmentioning

confidence: 99%

Lipid nanoparticle-based formulations for high-performance dentistry applications

et al. 2023

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Section: Dental Pulp Dentin and Alveolar Bone Regenerationmentioning

confidence: 99%

Lipid nanoparticle-based formulations for high-performance dentistry applications

et al. 2023

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“…The biological effects of TGF- β 1 on cells are contextual [ 23 ], depending on the culture medium, pretreatment procedures, incubation conditions, and more importantly, cell type and differentiation stage [ 24 , 25 ]. Furthermore, studies have shown that TGF- β 1 exerts different functionality during odontogenic differentiation [ 15 , 19 , 26 ]. Our study is now the first report to describe the dual role of TGF- β 1 in DPSC differentiation, and now shows that TGF- β 1 (at ˂5 ng/mL) facilitated early phases and modulated later phases of differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on the effect of TGF- β 1 on dentinogenesis and mineralization have generated contrasting data. Reportedly, TGF- β 1 can induce odontogenic differentiation of DPSCs and odontoblast-like cells in vitro [ 15 , 16 ]. Indeed, it has been shown that capping pulp with a TGF- β 1-containing material can induce odontoblast-like formation in dog molars [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Stage-Dependent Regulation of Dental Pulp Stem Cell Odontogenic Differentiation by Transforming Growth Factor-β1

Bai

Liu

et al. 2022

Stem Cells International

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Transforming growth factor-β1 (TGF-β1) is an important multifunctional cytokine with dual effects on stem cell differentiation. However, the role of TGF-β1 on odontogenic differentiation of dental pulp stem cells (DPSCs) remains to be entirely elucidated. In the present study, we initially investigated the effect of TGF-β1 at a range of concentrations (0.1-5 ng/mL) on the proliferation, cell cycle, and apoptosis of DPSCs. Subsequently, to determine the effect of TGF-β1 on odontogenic differentiation, alkaline phosphatase (ALP) activity and Alizarin Red S (ARS) staining assays at different concentrations and time points were performed. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were used to determine the levels of odonto-/osteo-genic differentiation-related gene and protein expression, respectively. For in vivo studies, newly formed tissue was assessed by Masson’s trichrome and von Kossa staining. Data indicated that TGF-β1 inhibited DPSCs proliferation in a concentration-and time-dependent manner ( p < 0.05 ) and induced cell cycle arrest but did not affect apoptosis. ALP activity was enhanced, while ARS reduced gradually with increasing TGF-β1 concentrations, accompanied by increased expression of early marker genes of odonto-/osteo-genic differentiation and decreased expression of late-stage mineralization marker genes ( p < 0.05 ). ALP expression was elevated in the TGF-β1-treatment group until 14 days, and the intensity of ARS staining was attenuated at days 14 and 21 ( p < 0.05 ). Compared with the control group, abundant collagen but no mineralized tissues were observed in the TGF-β1-treatment group in vivo. Overall, these findings indicate that TGF-β1 promotes odontogenic differentiation of DPSCs at early-stage while inhibiting later-stage mineralization processes.

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“…The all-in-one qPCR primer used to quantify target expression was TGF-β1 (HQP018044), with GAPDH (HQP006940) used as a reference gene (GeneCopoeia, Guangzhou, China). TGF-β1 expression was detected by RT-PCR based on previous studies of TGF-β1 release [ 37 , 38 ]. HaCaT cells (2.5 × 1 0 5 cells/well, 500 μl) were added to a 24-well plate.…”

Section: Methodsmentioning

confidence: 99%

Amphibian-derived peptide homodimer OA-GL17d promotes skin wound regeneration through the miR-663a/TGF-β1/Smad axis

et al. 2022

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Background Amphibian-derived peptides exhibit considerable potential in the discovery and development of new therapeutic interventions for clinically challenging chronic skin wounds. MicroRNAs (miRNAs) are also considered promising targets for the development of effective therapies against skin wounds. However, further research in this field is anticipated. This study aims to identify and provide a new peptide drug candidate, as well as to explore the underlying miRNA mechanisms and possible miRNA drug target for skin wound healing. Methods A combination of Edman degradation, mass spectrometry and cDNA cloning were adopted to determine the amino acid sequence of a peptide that was fractionated from the secretion of Odorrana andersonii frog skin using gel-filtration and reversed-phase high-performance liquid chromatography. The toxicity of the peptide was evaluated by Calcein-AM/propidium iodide (PI) double staining against human keratinocytes (HaCaT cells), hemolytic activity against mice blood cells and acute toxicity against mice. The stability of the peptide in plasma was also evaluated. The prohealing potency of the peptide was determined by MTS, scratch healing and a Transwell experiment against HaCaT cells, full-thickness injury wounds and scald wounds in the dorsal skin of mice. miRNA transcriptome sequencing analysis, enzyme-linked immunosorbent assay, real-time polymerase chain reaction and western blotting were performed to explore the molecular mechanisms. Results A novel peptide homodimer (named OA-GL17d) that contains a disulfide bond between the 16th cysteine residue of the peptide monomer and the sequence ‘GLFKWHPRCGEEQSMWT’ was identified. Analysis showed that OA-GL17d exhibited no hemolytic activity or acute toxicity, but effectively promoted keratinocyte proliferation and migration and strongly stimulated the repair of full-thickness injury wounds and scald wounds in the dorsal skin of mice. Mechanistically, OA-GL17d decreased the level of miR-663a to increase the level of transforming growth factor-β1 (TGF-β1) and activate the subsequent TGF-β1/Smad signaling pathway, thereby resulting in accelerated skin wound re-epithelialization and granular tissue formation. Conclusions Our results suggest that OA-GL17d is a new peptide drug candidate for skin wound repair. This study emphasizes the importance of exogenous peptides as molecular probes for exploring competing endogenous RNA mechanisms and indicates that miR-663a may be an effective target for promoting skin repair.

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Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells

Cited by 16 publications

References 34 publications

Lipid nanoparticle-based formulations for high-performance dentistry applications

Lipid nanoparticle-based formulations for high-performance dentistry applications

Stage-Dependent Regulation of Dental Pulp Stem Cell Odontogenic Differentiation by Transforming Growth Factor-β1

Amphibian-derived peptide homodimer OA-GL17d promotes skin wound regeneration through the miR-663a/TGF-β1/Smad axis

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