Liposomes in autoimmune diseases: selected applications in immunotherapy and inflammation detection

Crommelin, Daan J.A.; Rensen, A. J. M. L. Van; Wauben, Marca H. M.; Storm, Gert

doi:10.1016/s0168-3659(99)00044-9

Cited by 15 publications

(10 citation statements)

References 7 publications

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“…Moreover, these liposomes have the ability to leak from blood vessels, reaching the region of interest. [33][34][35][36] In contrast, large liposomes are not retained in infectious sites, as they are rapidly cleared from circulation by the organs of the mononuclear phagocyte system. 15 Therefore, the small size of the liposomes used in the study was adequate for the scintigraphic detection of the infection sites.…”

Section: Liposome Characterization Non-bt Liposomesmentioning

confidence: 99%

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Ferreira¹,

Boratto²,

Cardoso³

et al. 2015

IJN

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Osteomyelitis is a progressive destruction of bones caused by microorganisms. Inadequate or absent treatment increases the risk of bone growth inhibition, fractures, and sepsis. Among the diagnostic techniques, functional images are the most sensitive in detecting osteomyelitis in its early stages. However, these techniques do not have adequate specificity. By contrast, radiolabeled antibiotics could improve selectivity, since they are specifically recognized by the bacteria. The incorporation of these radiopharmaceuticals in drug-delivery systems with high affinity for bones could improve the overall uptake. In this work, long-circulating and alendronate-coated liposomes containing 99m technetium-radiolabeled ceftizoxime were prepared and their ability to identify infectious foci (osteomyelitis) in animal models was evaluated. The effect of the presence of PEGylated lipids and surface-attached alendronate was evaluated. The bone-targeted long-circulating liposomal 99m technetium–ceftizoxime showed higher uptake in regions of septic inflammation than did the non-long-circulating and/or alendronate-non-coated liposomes, showing that both the presence of PEGylated lipids and alendronate coating are important to optimize the bone targeting. Scintigraphic images of septic or aseptic inflammation-bearing Wistar rats, as well as healthy rats, were acquired at different time intervals after the intravenous administration of these liposomes. The target-to-non-target ratio proved to be significantly higher in the osteomyelitis-bearing animals for all investigated time intervals. Biodistribution studies were also performed after the intravenous administration of the formulation in osteomyelitis-bearing animals. A significant amount of liposomes were taken up by the organs of the mononuclear phagocyte system (liver and spleen). Intense renal excretion was also observed during the entire experiment period. Moreover, the liposome uptake by the infectious focus was significantly high. These results show that long-circulating and alendronate-coated liposomes containing 99m technetium-radiolabeled ceftizoxime have a tropism for infectious foci.

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Section: Liposome Characterization Non-bt Liposomesmentioning

confidence: 99%

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Ferreira¹,

Boratto²,

Cardoso³

et al. 2015

IJN

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“…The small diameter of 99m Tc-SpHL was appropriate for use in the identification of inflammatory foci. The small liposomes can take advantage of the vascular permeability increase and higher blood supply in the inflamed region where their escape occurred [20,21]. In contrast, large liposomes are not retained in inflamed sites as they are rapidly cleared from circulation by the organs of the mononuclear phagocytic system [22].…”

Section: Discussionmentioning

confidence: 99%

Biodistribution study and identification of inflammation sites using 99mTc-labelled stealth pH-sensitive liposomes

Carmo

Ferrari²,

Reis

et al. 2008

Nuclear Medicine Communications

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“…The mechanism of accumulation in the infectious sites of radiolabelled liposomes is by leakage of the vesicles through vessels due increased vascular permeability and subsequent phagocythosis by macrophages of the infected tissue (Goins et al, 1993;Erdogan et al, 2000;Laverman et al, 2001). Moreover, in the inflamed tissue the blood vessel presents endothelial junctions allowing the escape of the particles smaller that 200 nm from the blood circulation (Crommelin et al, 1999). (Awasthi et al, 2003;Crommelin et al, 1999;Erdogan et al, 2000;Boerman et al, 1997;Litzinger et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, in the inflamed tissue the blood vessel presents endothelial junctions allowing the escape of the particles smaller that 200 nm from the blood circulation (Crommelin et al, 1999). (Awasthi et al, 2003;Crommelin et al, 1999;Erdogan et al, 2000;Boerman et al, 1997;Litzinger et al, 1994). In contrast, large liposomes are not retained in infectious sites since that they are rapidly cleared from circulation by the organs of the mononuclear phagocyte system (Oyen et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Technetium-99m-labeled stealth pH-sensitive liposomes: a new strategy to identify infection in experimental model

Carmo

Oliveira

Mota

et al. 2007

Braz. arch. biol. technol.

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Liposomes in autoimmune diseases: selected applications in immunotherapy and inflammation detection

Cited by 15 publications

References 7 publications

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Biodistribution study and identification of inflammation sites using 99mTc-labelled stealth pH-sensitive liposomes

Technetium-99m-labeled stealth pH-sensitive liposomes: a new strategy to identify infection in experimental model

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