Liposomes containing lipids from Sulfolobus islandicus withstand intestinal bile salts: An approach for oral drug delivery?

Jensen, Sara Munk; Christensen, Camilla Jahn; Petersen, Julie Maria; Treusch, Alexander H.; Brandl, Martin

doi:10.1016/j.ijpharm.2015.07.026

Cited by 25 publications

(13 citation statements)

References 31 publications

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“…Preparation of liposomes, composed of tetraether lipids and DOTAP, was conducted at 5 mol% to take advantage of the stabilising effect of tetraether lipids, which are incorporated into the lipid bilayer ( Figure 2 ). A similar observation was also made by Jensen et al [ 22 ] where the highest fraction of tetraether lipid was 18 mol% in order to get stabilised liposomes. Furthermore, Parmentier et al [ 23 ] stated that a molar fraction of 5–15 mol% hGDNT was sufficient in delivering sensitive peptides through the GIT.…”

Section: Discussionsupporting

confidence: 84%

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids fromSulfolobus acidocaldarius

Engelhardt

Pinnapireddy

Baghdan

et al. 2017

Archaea

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Lipid vectors are commonly used to facilitate the transfer of nucleic acids into mammalian cells. In this study, two fractions of tetraether lipids from the archaea Sulfolobus acidocaldarius were extracted and purified using different methods. The purified lipid fractions polar lipid fraction E (PLFE) and hydrolysed glycerol-dialkyl-nonitol tetraether (hGDNT) differ in their structures, charge, size, and miscibility from conventional lipids. Liposomes were prepared by mixing tetraether lipids with cholesterol (CH) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) resulting in stable vectors for gene delivery. Lipoplexes were prepared by complexation of liposomes with a luciferase expressing plasmid (pCMV-luc) at certain nitrogen-to-phosphorus (N/P) ratios and optimised for the transient transfection of ovarian adenocarcinoma cells (SK-OV-3). Complexation efficacy was investigated by gel-red fluorescence assay. Biophysical properties, like size, surface charge, and morphology, were investigated by differential light scattering (DLS), atomic force microscopy (AFM), and scanning electron microscopy (Cryo-SEM), respectively, revealing structural differences between liposomes and lipoplexes. A range of stable transfecting agents containing tetraether lipids were obtained by incorporating 5 mol% of tetraether lipids. Lipoplexes showed a decrease in free gel-red with increasing N/P ratios indicating efficient incorporation of plasmid DNA (pDNA) and remarkable stability. Transfection experiments of the lipoplexes revealed successful and superior transfection of SK-OV-3 cell line compared to the commercially available DOTAP and branched polyethyleneimine (25 kDa bPEI).

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Section: Discussionsupporting

confidence: 84%

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids fromSulfolobus acidocaldarius

Engelhardt

Pinnapireddy

Baghdan

et al. 2017

Archaea

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“…As a group, they comprise species across wide temperature ranges for growth (from eurypsychrophilic to thermophiles), with the ability to exploit organic and/or inorganic electron donors and carbon sources, and can utilize molecular oxygen, ferric iron, and sulfate as electron acceptors. Acidophilic microorganisms have generated considerable interest as: (i) they catalyze the dissolution of sulfide minerals for recovery of valuable metals, termed “biomining” or “bioleaching” (Vera et al, 2013); (ii) they can cause uncontrolled sulfide mineral oxidation leading to the release of toxic, acidic and metal containing waters, called “acid mine drainage” (Mendez-Garcia et al, 2015); (iii) they are a source of extremozymes for use in biotechnologies (Elleuche et al, 2014); (iv) liposomes from these species have been investigated as a method for drug delivery (Jensen et al, 2015); and (v) these microorganisms may be analogs for early life on earth and potential life on other planets (Bauermeister et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Multiple Osmotic Stress Responses in Acidihalobacter prosperus Result in Tolerance to Chloride Ions

et al. 2017

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Extremely acidophilic microorganisms (pH optima for growth of ≤3) are utilized for the extraction of metals from sulfide minerals in the industrial biotechnology of “biomining.” A long term goal for biomining has been development of microbial consortia able to withstand increased chloride concentrations for use in regions where freshwater is scarce. However, when challenged by elevated salt, acidophiles experience both osmotic stress and an acidification of the cytoplasm due to a collapse of the inside positive membrane potential, leading to an influx of protons. In this study, we tested the ability of the halotolerant acidophile Acidihalobacter prosperus to grow and catalyze sulfide mineral dissolution in elevated concentrations of salt and identified chloride tolerance mechanisms in Ac. prosperus as well as the chloride susceptible species, Acidithiobacillus ferrooxidans. Ac. prosperus had optimum iron oxidation at 20 g L−1 NaCl while At. ferrooxidans iron oxidation was inhibited in the presence of 6 g L−1 NaCl. The tolerance to chloride in Ac. prosperus was consistent with electron microscopy, determination of cell viability, and bioleaching capability. The Ac. prosperus proteomic response to elevated chloride concentrations included the production of osmotic stress regulators that potentially induced production of the compatible solute, ectoine uptake protein, and increased iron oxidation resulting in heightened electron flow to drive proton export by the F0F1 ATPase. In contrast, At. ferrooxidans responded to low levels of Cl− with a generalized stress response, decreased iron oxidation, and an increase in central carbon metabolism. One potential adaptation to high chloride in the Ac. prosperus Rus protein involved in ferrous iron oxidation was an increase in the negativity of the surface potential of Rus Form I (and Form II) that could help explain how it can be active under elevated chloride concentrations. These data have been used to create a model of chloride tolerance in the salt tolerant and susceptible species Ac. prosperus and At. ferrooxidans, respectively.

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“…Hereby, the combination of two centrifugal forces gives vigorous agitation of a mixture of cells and beads (Massing et al, 2008). DAC has been introduced by Jensen et al for the disruption of archaeal cells (Jensen et al, 2015a).…”

Section: Cell Disruptionmentioning

confidence: 99%

“…Generally, liposomes including archaeosomes and mixed vesicles were prepared by lipid hydration followed by filter extrusion giving unilamellar vesicles (ULV), or bath sonication giving multilamellar vesicles (MLV) (Benvegnu et al, 2005a;Parmentier et al, 2011a;Parmentier et al, 2011b;Patel et al, 2000). For a protocol using sequential filter extrusion see (Jensen et al, 2015a), with factors of importance for the outcome of filter extrusion are described in (Hinna et al, 2016). In a recent study liposomes were prepared by dual asymmetric centrifugation (Parmentier et al, 2014).…”

Section: Liposome Preparationmentioning

confidence: 99%

“…To investigate liposomal stability in vitro, liposomes loaded with a marker ( 14 C-sucrose, 5(6)-carboxyfluorescein (CF) and fluorescein isothiocyanate (FITC)-dextran) are used as described in Section 3.2. Nonencapsulated marker was removed from the prepared liposomes by centrifugation, ultracentrifugation, Sephadex® G75 column chromatography or size exclusion chromatography (Benvegnu et al, 2005a;Jensen et al, 2015a;Li et al, 2010;Parmentier et al, 2011a;Patel et al, 2000). Experiments normally are conducted at 37°C.…”

Section: In Vitro Stability Screening Of Liposomes Containing Archaeamentioning

confidence: 99%

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Archaeal lipids in oral delivery of therapeutic peptides

Jacobsen

Jensen

Fricker

et al. 2017

European Journal of Pharmaceutical Sciences

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Archaea contain membrane lipids that differ from those found in the other domains of life (Eukarya and Bacteria). These lipids consist of isoprenoid chains attached via ether bonds to the glycerol carbons at the sn-2,3 positions. Two types of ether lipids are known, polar diether lipids and bipolar tetraether lipids. The inherent chemical stability and unique membrane-spanning characteristics of tetraether lipids render them interesting for oral drug delivery purposes. Archaeal lipids form liposomes spontaneously (archaeosomes) and may be incorporated in conventional liposomes (mixed vesicles). Both types of liposomes are promising to protect their drug cargo, such as therapeutic peptides, against the acidic environment of the stomach and proteolytic degradation in the intestine. They appear to withstand lipolytic enzymes and bile salts and may thus deliver orally administered therapeutic peptides to distant sections of the intestine or to the colon, where they may be absorbed, eventually by the help of absorption enhancers. Archaeal lipids and their semisynthetic derivatives may thus serve as biological source for the next generation oral drug delivery systems. The aim of this review is to present a systematic overview over existing literature on archaea carrying diether and tetraether lipids, lipid diversity, means of lipid extraction and purification, preparation and in vitro stability studies of archaeal lipid-based liposomal drug carriers and in vivo proof-of concepts studies.

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Liposomes containing lipids from Sulfolobus islandicus withstand intestinal bile salts: An approach for oral drug delivery?

Cited by 25 publications

References 31 publications

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids fromSulfolobus acidocaldarius

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids fromSulfolobus acidocaldarius

Multiple Osmotic Stress Responses in Acidihalobacter prosperus Result in Tolerance to Chloride Ions

Archaeal lipids in oral delivery of therapeutic peptides

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