Liposomes as antigen carriers and adjuvants in vivo

Buiting, A. M. J.; Rooijen, Nico van; Claassen, Eric

doi:10.1016/0923-2494(92)80066-t

Cited by 24 publications

(1 citation statement)

References 59 publications

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“…Although liposomes permit efficient antigen presentation to antigen-presenting cells and exhibit a depot-forming property at the injection site (110,111), we improved the immunogenicity of the gp41 int -Cys liposomes by the combination of two adjuvants, an anionic polymer (Carbopol-971P) and an oil emulsion (MF59). Carbopol-971P is commonly used for oral or topical application, and MF59 is a licensed adjuvant for influenza vaccine; both can be produced with the good manufacturing practice standard for potential clinical use.…”

Section: Discussionmentioning

confidence: 99%

A Fusion Intermediate gp41 Immunogen Elicits Neutralizing Antibodies to HIV-1

Lai

Hock

Radzimanowski

et al. 2014

Journal of Biological Chemistry

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Background: HIV-1 gp41 MPER is a target for inducing broadly neutralizing antibodies. Results: Gp41 int folds into a compact elongated structure that induces neutralizing antibodies upon immunization. Conclusion: Presentation of gp41 int in a lipid environment is beneficial to induce neutralizing antibodies. Significance: Membrane-anchored gp41 int is a promising antigen to improve breadth and potency of anti-gp41 antibody responses.

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Section: Discussionmentioning

confidence: 99%

A Fusion Intermediate gp41 Immunogen Elicits Neutralizing Antibodies to HIV-1

Lai

Hock

Radzimanowski

et al. 2014

Journal of Biological Chemistry

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TLC—A rapid method for liposome characterization

Brǎiloiu

Saila

Huhurez

et al. 1994

Biomedical Chromatography

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One of the most important problems for the use of liposomes as a drug delivery system is the modification of the vesicle induced by the liquid medium in which they are introduced (blood plasma for in vivo studies and the saline buffer solution for in vitro studies). Using thin-layer chromatography (TLC) we compared the behaviour of phosphatidylcholine (used for liposomes preparation) to that of the following unfilled liposomes: multilamellar liposomes (MLV); small unilamellar vesicles (SUV); and reverse phase evaporation vesicles (REV), before and after storage for 15 min in Krebs-Henseleit solution (37 degrees C, pH 7.4, aerated continuously with 95% O2 + 5% CO2). All variants contained the same amount of phosphatidylcholine. Thin-layer chromatography was performed on silica gel 60 as adsorbent. Two types of solvents were tested: one based on chloroform/alcohol (n-butanol or n-propanol or methanol)/water mixture (in different ratios) and another based on alcohol/alcohol/water mixture (n-butanol/n-propanol/water in 4/3/3 volume ratio). In all variants of chloroform containing solvents no differences were found between phosphatidylcholine and all types of liposomes. When using as solvent n-butanol/n-propanol/water significant differences were found between all types of liposomes before and after storage in Krebs-Henseleit solution. Their presence, after TLC treatment, was shown in electron microscopy studies.

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Liposomal Presentation of Antigens for Human Vaccines

Glück

1995

Pharmaceutical Biotechnology

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Liposomes as antigen carriers and adjuvants in vivo

Cited by 24 publications

References 59 publications

A Fusion Intermediate gp41 Immunogen Elicits Neutralizing Antibodies to HIV-1

A Fusion Intermediate gp41 Immunogen Elicits Neutralizing Antibodies to HIV-1

TLC—A rapid method for liposome characterization

Liposomal Presentation of Antigens for Human Vaccines

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