Liposome Solubilization Induced by Surfactant Molecules in a Microchip

Abstract

The dynamics of liposome solubilization was monitored by dynamic light scattering and optical microscopy. A newly designed Y-shape microchannel connected to a room was incorporated into a microchip and the reaction processes of the liposome suspension and surfactant solution were observed in the room after mixing the two fluids and stopping the flow. By using this microchip, we succeeded in real-time monitoring of liposome solubilization and the following dynamic processes of solubilization were proposed: 1) Deformed liposomes become spherical.2) The liposome size increases until the surfactant/liposome ratio in the liposome membrane reaches a threshold value. 3) Mixed micelles of surfactants and phospholipids are released and the liposomes collapse.

Amphiphilic Polypeptoids Serve as the Connective Glue to Transform Liposomes into Multilamellar Structures with Closely Spaced Bilayers

“…When a small fraction (<5%) of alkyl groups are present on long-chain polymers such as hydrophobically modified chitosan (hm-chitosan), the liposomes are connected into a network by the polymer chains . Hydrophobic interactions are intrinsic in understanding the effects of surfactants on lipid bilayers, where liposomes and cell membranes have been found to break down through fluidization of the bilayer and eventual dissolution of the lipids into mixed lipid–surfactant micelles. − …”

Anomalous Solubilization Behavior of Dimyristoylphosphatidyl-choline Liposomes Induced by Sodium Dodecyl Sulfate Micelles

“…In our previous study 23 we developed a system to monitor liposome-surfactant reactions; this involved mixing the solutions inside a microchip, and making observations with an optical microscope. The use of a "room-embedded" microchip (which has a wider channel compared with that of a conventional microchip in the middle of the channel, and in which the solution flow rate rapidly decreases when the flow comes into the "room") meant that the flow and stop procedures could be controlled more easily than with a conventional microchip.…”

Fabrication of Molecular Blocks with High Responsiveness to the Cancer Microenvironment by Ursodeoxycholic Acid

“…It has been reported that hydrophobic bile acids induce cell death via apoptosis signaling pathways 20 and disruption of the cell membrane by releasing mixed micelles of surfactants and phosphate lipids after insertion into the cell membrane. 19,36 On the other hand, hydrophilic bile acids have cytoprotective activity as inhibit the apoptosis pathway. 21 The hydrophobicity of bile acids used in this study was reported to be in the order of UDCA < CA < CDCA < DCA in both ionized and protonated states.…”

“…They are generally biosynthesized from cholesterol in the liver and play roles in various physiological processes such as digestion, glucose regulation, lipid metabolism and antibacterial defense in the small intestine. [16][17][18] They are also one of the amphiphilic molecules, causing cell death induction by releasing mixed micelles of surfactants and phosphate lipids by incorporation into the cell membrane, 19 or cell apoptosis via cell death signaling. 20,21 Recently, the cytotoxicity of hydrophobic bile acids has been exploited in the development of novel cancer drugs, and several bile acid derivatives have shown antiproliferative activities against a wide variety of human cancer cell lines.…”