Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Venero, Orion M; Sato, Wakana; Heili, Joseph M; Deich, Christopher; Adamala, Katarzyna P.

doi:10.1007/978-1-0716-1998-8_14

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…Liposomes were prepared according to the DSCF protocol, using a 3D printed device described before. 39 Briefly, thin lipid films prepared by evaporating chloroform from a mixture of lipids were dissolved in mineral oil. A microcapillary was fitted into the 3D printed microfluidic device.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

et al. 2023

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Recently, a new subset of fluorescent proteins has been identified from the Aequorea species of jellyfish. These fluorescent proteins were characterized in vivo; however, there has not been validation of these proteins within cell-free systems. Cell-free systems and technology development is a rapidly expanding field, encompassing foundational research, synthetic cells, bioengineering, biomanufacturing, and drug development. Cell-free systems rely heavily on fluorescent proteins as reporters. Here we characterize and validate this new set of Aequorea proteins for use in a variety of cell-free and synthetic cell expression platforms.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

et al. 2023

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“…Liposomes were prepared according to the DSCF protocol, using 3D printed device described before. 33…”

Section: Methodsmentioning

confidence: 99%

“…Liposomes were prepared according to the DSCF protocol, using 3D printed device described before. 33 Briefly, thin lipid films prepared by evaporating chloroform from mixture of lipids were dissolved in mineral oil. Microcapillary was fitted into the 3D printed microfluidic device.…”

Section: Liposomesmentioning

confidence: 99%

NewAequoreafluorescent proteins for cell-free bioengineering

Deich

Gaut

Sato

et al. 2022

Preprint

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Recently, a new subset of fluorescent proteins has been identified from the Aequorea species of jellyfish. These fluorescent proteins were characterized in vivo; however, there has not been validation of these proteins within cell-free systems. Cell-free systems and technology development is a rapidly expanding field, encompassing foundational research, synthetic cells, bioengineering, biomanufacturing and drug development. Cell-free systems rely heavily on fluorescent proteins as reporters. Here we characterize and validate this new set of Aequorea proteins for use in a variety of cell-free and synthetic cell expression platforms.

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“…By further increasing the number of nested stages, functional particles with more complex structures can also be produced. [ 71 ] However, it requires extremely high dimensional accuracy and cleanliness of manufacturing instruments, and the glass microchannels are easily blocked during the experimental operation. Considering the fragile of glass capillaries, the manual fabrication with microforge and pipette puller restricts production quantity at a time during assembly.…”

Section: Droplet Microfluidics Devicesmentioning

confidence: 99%

Recent Progress of Droplet Microfluidic Emulsification Based Synthesis of Functional Microparticles

et al. 2023

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The remarkable control function over the functional material formation process enabled by droplet microfluidic emulsification approaches can lead to the efficient and one‐step encapsulation of active substances in microparticles, with the microparticle characteristics well regulated. In comparison to the conventional fabrication methods, droplet microfluidic technology can not only construct microparticles with various shapes, but also provide excellent templates, which enrich and expand the application fields of microparticles. For instance, intersection with disciplines in pharmacy, life sciences, and others, modifying the structure of microspheres and appending functional materials can be completed in the preparation of microparticles. The as‐prepared polymer particles have great potential in a wide range of applications for chemical analysis, heavy metal adsorption, and detection. This review systematically introduces the devices and basic principles of particle preparation using droplet microfluidic technology and discusses the research of functional microparticle formation with high monodispersity, involving a plethora of types including spherical, nonspherical, and Janus type, as well as core–shell, hole–shell, and controllable multicompartment particles. Moreover, this review paper also exhibits a critical analysis of the current status and existing challenges, and outlook of the future development in the emerging fields has been discussed.

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Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Cited by 8 publications

References 15 publications

New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

NewAequoreafluorescent proteins for cell-free bioengineering

Recent Progress of Droplet Microfluidic Emulsification Based Synthesis of Functional Microparticles

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