Liposome-enhanced transformation of streptococcus lactis and plasmid transfer by intergeneric protoplast fusion of streptococcus lactis and bacillus subtilis

Vossen, J Van der

doi:10.1016/0378-1097(88)90318-7

Cited by 10 publications

(12 citation statements)

References 15 publications

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“…1). Similar results were lo3 -lo2 - achieved by Van der Vossen et al (1988), who found 22.5% PEG 6000 to be the best concentration for transformation of Strep. lactis IL1403 and Strep.…”

Section: Resultssupporting

confidence: 77%

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Transformation of Streptococcus bovis protoplasts by plasmid DNA

Mareková

Kmet

Javorský

1996

Lett Appl Microbiol

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The transformation and subsequent regeneration of ruminal strain Streptococcus bovis AO24/85 protoplasts by plasmid DNA was studied. The best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. Optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. Addition of Ca2+ and Mg2+ ions (2.5 mmol l-1) to the transformation medium increased the proportion of regenerated cells. Transformation frequencies were 3 x 10(3) transformants per microgram of pNZ12 and 2.4 x 10(2) per microgram of pJK108, respectively.

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“…1). Similar results were lo3 -lo2 - achieved by Van der Vossen et al (1988), who found 22.5% PEG 6000 to be the best concentration for transformation of Strep. lactis IL1403 and Strep.…”

Section: Resultssupporting

confidence: 77%

“…Polyethylene glycol (PEG) 6000 is a frequently used inductor of transformation (Van der Vossen et al 1988). Our experiments with graduated concentrations of 20-35% PEG 6000 in the transformation medium revealed that 25% PEG 6000 gave the best results with protoplasts of Strep.…”

Section: Resultsmentioning

confidence: 87%

Transformation of Streptococcus bovis protoplasts by plasmid DNA

Mareková

Kmet

Javorský

1996

Lett Appl Microbiol

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“…lactis protoplasts with plasmid DNA was reported in 1982 [13]. Since that time, several modified transformation protocols have been described [14][15][16][17][18], including the now generally preferred method of electrotransformation [19][20][21][22]. Plasmid-free host strains became available such as the widely used L. lactis ssp.…”

Section: Gene Expression In Lactococcus Lactismentioning

confidence: 99%

“…lactis. Again, data obtained from this kind of experiments should be interpreted with care, because of the indirect mode of measurement of transcript formation: (1-5), P21, P23, P32, P44, P59 [38]; (6-10), PI, PI0, P21, ss45, ss80 [30]; (11,12), pWV05 prtP, prtM [39,40,97l; (13), nisin precursor [98]; (14, 14a), p9BA-60RFAI = IcnMa, ORFB1 =IcnA ([42], M. van Belkum personal communication); (15), usp45 [43]; (16), lacR [99]; (17)(18)(19)(20)(21), Pal, Pf2, Pa3, Pg2, Pfl [41l. Promoters (17)(18)(19)(20)(21) are lactococcal phage promoters, The proposed -35…”

Section: Cloning and Characterization Of Lactococcal Expression Signamentioning

confidence: 99%

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Gene expression in Lactococcus lactis

Guchte

Kok

Venema

1992

FEMS Microbiology Letters

105

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Lactic acid bacteria are of major economic importance, as they occupy a key position in the manufacture of fermented foods. A considerable body of research is currently being devoted to the development of lactic acid bacterial strains with improved characteristics, that may be used to make fermentations pass of more efficiently, or to make new applications possible. Therefore, and because the lactococci are designated ‘GRAS’ organisms (‘generally recognized as safe’) which may be used for safe production of foreign proteins, detailed knowledge of homologous and heterologous gene expression in these organisms is desired. An overview is given of our current knowledge concerning gene expression in Lactococcus lactis. A general picture of gene expression signals in L. lactis emerges that shows considerable similarity to those observed in Escherichia coli and Bacillus subtilis. This feature allowed the expression of a number of L. lactis‐derived genes in the latter bacterial species. Several studies have indicated, however, that in spite of the similarities, the expression signals from E. coli, B. subtilis and L. lactis are not equally efficient in these three organisms.

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Gene transfer systems and transposition

Gasson¹,

Fitzgerald²

1994

Genetics and Biotechnology of Lactic Acid Bacteria

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Liposome-enhanced transformation of streptococcus lactis and plasmid transfer by intergeneric protoplast fusion of streptococcus lactis and bacillus subtilis

Cited by 10 publications

References 15 publications

Transformation of Streptococcus bovis protoplasts by plasmid DNA

Transformation of Streptococcus bovis protoplasts by plasmid DNA

Gene expression in Lactococcus lactis

Gene transfer systems and transposition

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