Liposome encapsulation of a soluble recombinant fragment of the respiratory syncytial virus (RSV) G protein enhances immune protection and reduces lung eosinophilia associated with virus challenge

Mader, David; Huang, Yan; Wang, Cheng; Fraser, Robert; Issekutz, Andrew C.; Stadnyk, Andrew W.; Anderson, Robert

doi:10.1016/s0264-410x(99)00373-4

Cited by 24 publications

(13 citation statements)

References 44 publications

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“…Since we and others have utilized prokaryotically expressed fragments of the RSV G protein as vaccine candidates (16,23,27,29), the ability of such fragments to protect against RSV challenge must be tempered with their potential to initiate harmful inflammatory responses. As we previously reported (16), wild-type Trx-G128-229 in fact sensitized mice to pulmonary eosinophilia following RSV challenge (Fig.…”

Section: Fig 1 Effects Of G-protein Mutations On the Induction Of Smentioning

confidence: 99%

“…Encapsulation of Trx-G proteins within liposomes, for example, increases protection against RSV while suppressing eosinophilia (15,23). Clearly, there are potential opportunities for new developments in molecular engineering as well as immunization delivery in the realization of a successful RSV vaccine.…”

Section: Fig 1 Effects Of G-protein Mutations On the Induction Of Smentioning

confidence: 99%

“…Thioredoxin (Trx) fusion proteins of RSV G or dengue virus type 2 E protein sequences were prepared as previously described (23). Briefly, the above-described RSV G or dengue virus type 2 E protein sequences contained in modified pET-32-LIC plasmids were expressed as Trx fusion proteins in transformed Escherichia coli BL21/DE3 cells after induction with isopropyl-␤-D-thiogalactopyranoside.…”

mentioning

confidence: 99%

“…Fourteen days after the second dose, mice were challenged intranasally with RSV (2 ϫ 10 6 PFU in 50 l). Mice were sacrificed by using sodium pentobarbital 4 days later and assayed for titers of virus in lung and leukocyte infiltration in bronchoalveolar fluids as previously described (16,23). Data were analyzed using the GraphPad (San Diego, Calif.) Instat software package, using analysis of variance by the Kruskal-Wallis test.…”

mentioning

confidence: 99%

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Modulation of Protective Immunity, Eosinophilia, and Cytokine Responses by Selective Mutagenesis of a Recombinant G Protein Vaccine against Respiratory Syncytial Virus

Huang

Anderson

2005

J Virol

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Using an Escherichia coli-grown plasmid vector encoding a fragment of thioredoxin (Trx) fused to a central region (amino acids 128 to 229) of the respiratory syncytial virus (RSV) (Long strain) G protein, we employed site-directed mutagenesis to investigate the importance of selected amino acids to vaccine efficacy. Mice were immunized with a total of 10 wild-type or mutant Trx-G proteins and challenged intranasally with RSV. Striking differences in the induction of RSV G-protein-specific antibodies, protection against RSV challenge, cytokine RNA responses, and induction of RSV-associated eosinophilic inflammation were observed among the mutant proteins examined. Taken together, the results identify a critical role for specific amino acids in the induction of protective immunity and priming for eosinophilia against RSV.

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Section: Fig 1 Effects Of G-protein Mutations On the Induction Of Smentioning

confidence: 99%

Section: Fig 1 Effects Of G-protein Mutations On the Induction Of Smentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Modulation of Protective Immunity, Eosinophilia, and Cytokine Responses by Selective Mutagenesis of a Recombinant G Protein Vaccine against Respiratory Syncytial Virus

Huang

Anderson

2005

J Virol

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“…Adjuvants may also act as delivery vehicles for antigens, resulting in the targeting of antigens to immuno-competent cells. Examples include liposomes [68][69][70][71], virosomes [72,73], oil adjuvants, small (!10 Ìm) PLG microspheres and ISCOMs [74,75]. Finally, some compounds such as FCA [76], muramyl dipeptide [77], cholera toxin [78,79], MF59 (an oil based adjuvant) [80], lipopolysaccharide and certain CpG oligonucleotides [81] act as immunostimulators.…”

Section: Delivery Systemsmentioning

confidence: 99%

Recent Advances in the Development of Peptide Vaccines for Hepatitis B

Moynihan

Howard

2001

Intervirology

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The control of hepatitis B by vaccination is arguably one of medicine’s greatest achievements in terms of protecting infants and adults at high risk of infection. Paradoxically, however, the existence of a large reservoir of chronically infected individuals will not diminish the risk of infection by those coming into close contact with such persons until universal infant immunisation is practised globally and vaccines are in place to ensure maximum efficacy in those with impaired immune responses, immunity is achieved with fewer doses, and immunisation as an adjunct to the antiviral treatment of chronic carriers is adopted. These imperatives have continued to stimulate research into vaccines based on chemically synthesised short peptides, and those systems best suited for their delivery. This review discusses the potential of synthetic peptide formulations as efficient inducers of both humoral and cellular immune responses against hepatitis B, and reviews recent advances in peptide delivery. Synthetic peptide and delivery systems technologies will, amongst others, be of paramount importance in the global fight for the eradication of hepatitis B in the 21st century.

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New Approaches to Vaccine Delivery

Partidos¹

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Liposome encapsulation of a soluble recombinant fragment of the respiratory syncytial virus (RSV) G protein enhances immune protection and reduces lung eosinophilia associated with virus challenge

Cited by 24 publications

References 44 publications

Modulation of Protective Immunity, Eosinophilia, and Cytokine Responses by Selective Mutagenesis of a Recombinant G Protein Vaccine against Respiratory Syncytial Virus

Modulation of Protective Immunity, Eosinophilia, and Cytokine Responses by Selective Mutagenesis of a Recombinant G Protein Vaccine against Respiratory Syncytial Virus

Recent Advances in the Development of Peptide Vaccines for Hepatitis B

New Approaches to Vaccine Delivery

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