Liposome/DNA Systems: Correlation Between Hydrophobicity and DNA Conformational Changes

Chiaramoni, Nadia Silvia; Baccarini, L. C.; Taira, Marı́a Cristina; Alonso, Silvia del Valle

doi:10.1007/s10867-008-9103-2

Cited by 8 publications

(14 citation statements)

References 21 publications

(33 reference statements)

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“…Since photopolymerizable lipids combine the plasticity of lipids with the robustness of polymers, they have received much attention in several biological areas (AlonsoRomanowski et al 2003;Chiaramoni et al 2007Chiaramoni et al , 2008Chiaramoni et al , 2010Fernandez Ruocco et al 2013;Gasparri et al 2011). Lipopolymers have many potential applications in medicine because they present great stability, given by the photoactivable bonds in photopolymerizable lipids (Yavlovich et al 2010).…”

Section: Liposomesmentioning

confidence: 99%

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

et al. 2017

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In recent years, the use of commercial nanoparticles in different industry and health fields has increased exponentially. However, the uncontrolled application of nanoparticles might present a potential risk to the environment and health. Toxicity of these nanoparticles is usually evaluated by a fast screening assay in zebrafish (Danio rerio). The use of this vertebrate animal model has grown due to its small size, great adaptability, high fertilization rate and fast external development of transparent embryos. In this review, we describe the toxicity of different micro-and nanoparticles (carbon nanotubes, dendrimers, emulsions, liposomes, metal nanoparticles, and solid lipid nanoparticles) associated to their biophysical properties using this model. The main biophysical properties studied are size, charge and surface potential due to their impact on the environment and health effects. The review also discusses the correlation of the effects of the different nanoparticles on zebrafish. Special focus is made on morphological abnormalities, altered development and abnormal behavior. The last part of the review debates changes that should be made in future directions in order to improve the use of the zebrafish model to assess nanotoxicity.

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Section: Liposomesmentioning

confidence: 99%

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

et al. 2017

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“…These conformational changes could be followed through CD spectroscopy, but more importantly, the conformation/lipid combination was found to be a more effective cellular transfecting agent . Subsequent studies found that increased liposome hydrophobicity correlates with greater DNA transformation from B to C‐form . Standard DNA/liposomes have limited periods of intracellular viability and, in turn, limited chromosomal integration, thus it is potentially feasible that specific DNA nanostructures, especially if they have additional functionality, may provide the ideal combination of lipid formulation along with DNA composition to surpass these barriers.…”

Section: Intrinsically Active Dna Materialsmentioning

confidence: 99%

Utilizing the Organizational Power of DNA Scaffolds for New Nanophotonic Applications

Bui

Dı́az

Fontana

et al. 2019

Advanced Optical Materials

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Rapid development of DNA technology has provided a feasible route to creating nanoscale materials. DNA acts as a self‐assembled nanoscaffold capable of assuming any three‐dimensional shape. The ability to integrate dyes and new optical materials such as quantum dots and plasmonic nanoparticles precisely onto these architectures provides new ways to exploit their near‐ and far‐field interactions. A fundamental understanding of these optical processes will help drive development of next‐generation photonic nanomaterials. This review is focused on latest progress in DNA‐based photonic materials and highlights DNA scaffolds for rapidly assembling and prototyping nanoscale optical devices. Three areas are discussed including intrinsically active DNA structures displaying chiral properties, DNA scaffolds hosting plasmonic nanomaterials, and fluorophore‐labeled DNAs that engage in Förster resonance energy transfer and give rise to complex molecular photonic wires. An explanation of what is desired from these optical processes when harnessed sets the tone for what DNA scaffolds are providing toward each focus. Examples from the literature illustrate current progress along with a discussion of challenges to overcome for further improvements. Opportunities to integrate diverse classes of optically active molecules including light‐generating enzymes, fluorescent proteins, nanoclusters, and metal–chelates in new structural combinations on DNA scaffolds are also highlighted.

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“…The first two are the characteristic absorbance maxima when the membrane is in the gel phase, whereas the second two are the characteristic absorbance maxima when the membrane is in liquid crystalline phase [17]. The ratio of the absorbance at 570 nm with respect to that at 500 nm, called hydrophobicity factor (HF) [18], determines the degree of hydrophobic sites exposed to the interface.…”

Section: Surface Modificationsmentioning

confidence: 99%

“…Hydrophobic defects on the surface of lipid bilayers may be related to their stability because the higher water ingress increases liposome destabilization [18]. Such defects can be studied using the probe Merocyanine 540 (MC540).…”

Section: Hydrophobicity Factor (Hf)mentioning

confidence: 99%

“…When MC540 is in a hydrophobic environment, such as a liposome bilayer, the spectrum undergoes a shift to longer wavelengths. The ratio of absorbance at 570 nm and 500 nm is a parameter called Hydrophobicity Factor (HF) and gives an idea of the number of exposed hydrophobic sites at the water-liposome interface and the level of packing or rigidity on the liposome surface [17,18,21]. Samples were measured at 10°C and 37°C to evaluate changes in the membrane at the gel phase temperature (10°C) and physiological temperature (37°C).…”

Section: Hydrophobicity Factor (Hf)mentioning

confidence: 99%

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DSPC Liposomes Improve Transport of L-cysteine and Reduce Metabolic Activity

Perrotta

Prietto

Alonso

et al. 2016

BBJ

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Aims:In this work, we developed and characterized liposomal formulations that encapsulate Lcysteine to study their further application in drug delivery and amino acid supplementation. The lipids used were 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Methodology: Encapsulation efficiency and amino acid release were determined. For biophysical characterization of the three formulations, the size, surface charge and surface packing were also studied. Cell viability was analyzed with MTT reagent after treatments with formulations ir order to study efficiency of these systems in induce changes in metabolism. Results: Results showed that L-cysteine interacts at the polar head level and that this interaction stabilizes the surface charge and prevents aggregation. We also determined the influence on cell metabolism in all formulations. The presence of L-cysteine in the DSPC formulation induced deeper Perrotta et al.; BBJ, 12(4): 1-11, 2016; Article no.BBJ.24723 2 changes in metabolism, evidencing that this formulation provides better transport of this amino acid. Conclusion: Liposomes developed herein are well suited for the application in the delivery of L-cysteine. Particularly, they can encapsulate nearly all the L-cysteine and can retain it for 6 hours. Also, L-cysteine stabilized liposomes, preventing their aggregation. L-cysteine encapsulated in the DSPC formulation induced deeper changes in cell metabolism, causing a decrease in metabolic activity; this was probably due to a higher entry, thus a better liposome-mediated transport. Considering that the smaller the particle, the better the circulation, we believe that the stabilization of the vesicle by L-cysteine may allow these transporters to have higher circulation times. Based on the above, we conclude that the DSPC formulation is the best suited for further application in L-cysteine delivery. Original Research Article

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Liposome/DNA Systems: Correlation Between Hydrophobicity and DNA Conformational Changes

Cited by 8 publications

References 21 publications

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

Utilizing the Organizational Power of DNA Scaffolds for New Nanophotonic Applications

DSPC Liposomes Improve Transport of L-cysteine and Reduce Metabolic Activity

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