Liposome-DNA interaction: microcalorimetric study

Mrevlishvili, G. M.; Kankia, Besik; Mdzinarashvili, T. J.; Brelidze, Tinatin I.; Khvedelidze, Mariam; Metreveli, Nunu; Razmadze, G. Z.

doi:10.1016/s0009-3084(98)00019-x

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…Differential scanning calorimetry (DSC) analysis over a large temperature range has shown that, in an aqueous buffer solution, DNA undergoes a transition at temperatures of about 40 8C. [37,38] In our experiments, the temperature does not affect samples at low b-CD concentrations, at which the DNA remains in a compact state, as shown in Figure 3. Only the samples with DNA in its coil conformation show dramatic changes at about 40 8C, in good agreement with the DNA temperature transition that was obtained from heat capacity profiles by Mrevlishvilinet et al in 1998.…”

Section: Dna In Solutionmentioning

confidence: 51%

“…By using these techniques, we explore both the effect of b-CD concentration and temperature. [37,38] In our experiments, the temperature does not affect samples at low b-CD concentrations, at which the DNA remains in a compact state, as shown in Figure 3. The adiabatic compressibility has previously been used to explore conformational changes of proteins in solution [36] and, more recently, to investigate conformational changes of DNA.…”

Section: Dna In Solutionmentioning

confidence: 56%

“…With density and sound-velocity data, it is possible to directly calculate the adiabatic compressibility (b) of the solution by using the Laplace equation, b = 1/u 2 1, in which u is the sound velocity and 1 is the density, and, by knowing the temperature dependence of those parameters, we can estimate the expansibility. [37] These results are also an indication of the protective effect of the compacting agent against denaturation if the DNA is in a compact state. [19] Figure 3 shows the temperature dependence of density and These results show a parabolic growth and a decrease for the sound velocity and the density, respectively.…”

Section: Dna In Solutionmentioning

confidence: 96%

“…Only the samples with DNA in its coil conformation show dramatic changes at about 40 8C, in good agreement with the DNA temperature transition that was obtained from heat capacity profiles by Mrevlishvilinet et al in 1998. [37] These results are also an indication of the protective effect of the compacting agent against denaturation if the DNA is in a compact state. Figure 3 b shows two well-defined linear regions, in which the inflection point indicates a DNA globule-to-coil conformational transition.…”

Section: Dna In Solutionmentioning

confidence: 96%

See 3 more Smart Citations

A Versatile Approach towards the Compaction, Decompaction, and Immobilization of DNA at Interfaces by Using Cyclodextrins

González‐Pérez

Ruso

2013

ChemPhysChem

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We investigate the temperature dependence of interactions of β-cyclodextrin (CD)/hexadecyltrimethylammonium bromide (CTAB) self-assemblies with DNA during the decompaction of DNA/CTAB complexes. By combining direct imaging techniques with density and sound-velocity measurements, we can explain the decompaction process and suggest a suitable model. The DNA-decompaction process by using CDs is accompanied by interactions with surfaces, such as glass or mica. The mechanism of β-CD/CTAB self-assembly is elucidated and the immobilization of DNA onto negatively charged surfaces is explained. Differences between the fractal dimensions of DNA that is adsorbed onto the surfaces are related to strong and weak binding, which permit the partial relaxation of DNA on the surfaces. The β-CD/CTAB self-assembled monolayers are demonstrated to be a facile and efficient route for surface functionalization, which allows for the immobilization of biomacromolecules in close proximity without any intermediate binding or deprotection steps. Moreover, this route is expected to show several advantages that might contribute to improving the performance of future biosensors as gentle immobilization-limiting alteration of the protein structure, oriented immobilization, thereby allowing homogeneous accessibility, reversible immobilization, thereby allowing reutilizations, and high compatibility with various types of biomacromolecules.

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Section: Dna In Solutionmentioning

confidence: 51%

Section: Dna In Solutionmentioning

confidence: 56%

Section: Dna In Solutionmentioning

confidence: 96%

Section: Dna In Solutionmentioning

confidence: 96%

See 2 more Smart Citations

A Versatile Approach towards the Compaction, Decompaction, and Immobilization of DNA at Interfaces by Using Cyclodextrins

González‐Pérez

Ruso

2013

ChemPhysChem

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“…Straightforward analysis provides information on the enthalpy, the transition temperatures and the cooperativity of the lipoplex phases. In a classic 1998 study published in Chemistry and Physics of Lipids , Mrevlishvili et al 12 demonstrated that using DSC to study DNA-lipid membrane interactions provides important information for the orderdisorder lipid transition with and without DNA as well as for the helix-coil transition of DNA in the presence or not of the lipid membrane. Figure 8.1 shows characteristic data from this study.…”

Section: Dna-membrane Interaction Studies With Dscmentioning

confidence: 99%

DSC applications: nucleic acids and membrane interactions

Giatrellis

Nounesis

2013

Drug-Biomembrane Interaction Studies

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Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization

Edwards

Baeumner

2006

Anal Bioanal Chem

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A novel protocol for the synthesis of dye-encapsulating liposomes tagged with DNA oligonucleotides at their outer surface was developed. These liposomes were optimized for use as signal enhancement agents in lateral-flow sandwich-hybridization assays for the detection of single-stranded RNA and DNA sequences. Liposomes were synthesized using the reverse-phase evaporation method and tagged with oligonucleotides by adding cholesteryl-modified DNA probes to the initial lipid mixture. This resulted in a greatly simplified protocol that provided excellent control of the probe coverage on the liposomes and cut the preparation time from 16 hours to just 6 hours. Liposomes were prepared using probe concentrations ranging from 0.00077 to 0.152 mol% of the total lipid, several hydrophobic and polyethylene glycol-based spacers between the cholesteryl anchor and the probe, and liposome diameters ranging from 208 nm to 365 nm. The liposomes were characterized by dynamic light scattering, visible spectroscopy, and fluorescence spectroscopy. Their signal enhancement functionality was compared by using them in lateral-flow optical biosensors for the detection of single-stranded DNA sequences. In these assays, an optimal reporter probe concentration of 0.013 mol%, liposome diameter of 315 nm, and liposome optical density of 0.4-0.6 at 532 nm were found. The spacer length between the cholesteryl anchor and the probe showed no significant effect on the signals in the lateral-flow assays. The results presented here provide important data for the general use of liposomes as labels in analytical assays, with specific emphasis on nucleic acid detection via lateral flow assays.

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Liposome-DNA interaction: microcalorimetric study

Cited by 11 publications

References 7 publications

A Versatile Approach towards the Compaction, Decompaction, and Immobilization of DNA at Interfaces by Using Cyclodextrins

A Versatile Approach towards the Compaction, Decompaction, and Immobilization of DNA at Interfaces by Using Cyclodextrins

DSC applications: nucleic acids and membrane interactions

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization

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