Liposome-complexed adenoviral gene transfer in cancer cells expressing various levels of coxsackievirus and adenovirus receptor

Lee, E. M.; Hong, Seunghee; Lee, Y. J.; Kang, Yoon-A; Choi, Kang‐Yell; Choi, Stephanie; Kim, I. H.; Lim, Soo-Jeong

doi:10.1007/s00432-003-0521-z

Cited by 23 publications

(17 citation statements)

References 26 publications

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“…Adenoviral vector-mediated gene manipulation is a potential approach (Song et al, 2007), but CAR-deficiency in some cancer cells prevents effective adenoviral infection of target cells. We previously found to enhance the stability and uptake of liposome-complexed adenovirus in CAR deficient human cancer cell lines (Lee et al, 2004). In this study we demonstrated that the problem of CAR-deficiency, can be overcome by complexing the adenovirus with PEGPE in vitro as well as in vivo in a mouse metastasis model we established, by injecting syngenetic mice with CAR-deficient CT-26 mouse colon cancer cells.…”

Section: Discussionmentioning

confidence: 68%

“…We demonstrated previously that addition of PEGPE enhances the stability and uptake of liposomecomplexed adenovirus in CAR deficient human cancer cell lines (Lee et al, 2004). Here we investigated the effect of liposome dose on the efficiency of liposome-complexed adenoviral gene transfer in CAR deficient CT-26 cell lines.…”

Section: Adenoviral Gene Expression In Car Deficient Cells By Liposommentioning

confidence: 95%

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Gene transfer using liposome-complexed adenovirus seems to overcome limitations due to coxsackievirus and adenovirus receptor-deficiency of cancer cells, both in vitro and in vivo

Han

Lee²,

Jung

et al. 2008

Exp Mol Med

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Section: Discussionmentioning

confidence: 68%

Section: Adenoviral Gene Expression In Car Deficient Cells By Liposommentioning

confidence: 95%

Gene transfer using liposome-complexed adenovirus seems to overcome limitations due to coxsackievirus and adenovirus receptor-deficiency of cancer cells, both in vitro and in vivo

Han

Lee²,

Jung

et al. 2008

Exp Mol Med

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“…42 PEG incorporation into DOTAP liposome-enhanced stability and uptake of liposome-complexed adenoviral and further improved the infection efficiency regardless of infection conditions and reported that in CAR-deficient cells. In this study, we prepared PEG-PE cationic liposome to encapsulate adenovirus and confirmed that the resulting complex could enhance the transfection ability both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 93%

Suppression of ovarian cancer growth via systemic administration with liposome-encapsulated adenovirus-encoding endostatin

Yang

Wang

et al. 2009

Cancer Gene Ther

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Gene therapy using adenoviral vector containing the endostatin gene is a promising strategy for advanced cancers. However, host immune response to adenovirus and the lack of the requisite coxsackie-adenovirus receptor (CAR) in many primary cells limit the in vivo application. Liposome-complexed adenoviral vectors have proven to be useful for enhancing gene delivery in target cells that lack adenoviral receptors and avoiding a neutralizing antibody response. Here, we investigated antitumor effects of intravenous administration with PEG-PE cationic liposome-encapsulated recombinant human endostatin adenovirus (Ad-hEndo) on CAR-negative ovarian cancer. Electron micrography (EM) showed that these liposomes efficiently encapsulated the vectors, allowing CAR-independent adenovector transduction. The results showed that the complex enhanced transfection efficiency of recombinant adenovirus. Prolonged systemic administration was performed in immunocompetent mice and did not induce significant antibody response. The antitumor effect with PEG-PE cationic liposome encapsulated with Ad-hE (Ad-hE/lipo) was evaluated in the human ovarian cancer model. Systemic administration was well tolerated and resulted in marked suppression of tumor growth in an established ovarian cancer model, which was associated with a decreased number of micro-vessels and increased apoptosis of tumor cells. Our study shows that PEG-PE cationic liposome-encapsulated Ad-hE (Ad-hE/Lipo) can be administrated intravenously and lastingly to inhibit angiogenesis, thus showing promising clinical application.

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“…Simple mixing of adenoviral particles and cationic nanoparticles spontaneously produces complexes, and the Ad-nanoparticle complexes facilitate viral attachment to negatively charged cell membranes and increase gene transfer in both CAR-positive and CAR-negative cells. [7][8][9][10] The composition of a nanoparticle determines the ability of that nanoparticle to act as a carrier of chemicals and biologics. 11 The type and content of a cationic lipid and other components used to form cationic lipid-based nanoparticles can affect the surface charge, size, and structure of the nanoparticles, thereby varying the strength of the interactions between nanoparticles and viral particles.…”

Section: Introductionmentioning

confidence: 99%

“…12,13 A very limited number of studies, however, have addressed this issue in adenoviral gene delivery. Compared with liposomes containing 1,2-dioleyloxy-3-trimethylammonium propane chloride as a cationic lipid (Lipofectin ® ), dioleoyltrimethylaminopropane (DOTAP)-containing liposomes were found to be more effective in enhancing adenovirus-mediated transgene expression, 7 emphasizing the importance of the of cationic lipid's chemical structure.…”

Section: Introductionmentioning

confidence: 99%

Optimization and physicochemical characterization of a cationic lipid-phosphatidylcholine mixed emulsion formulated as a highly efficient vehicle that facilitates adenoviral gene transfer

Kim¹,

Lee²,

Kim³

et al. 2017

IJN

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Cationic lipid-based nanoparticles enhance viral gene transfer by forming electrostatic complexes with adenoviral vectors. We recently demonstrated the superior complexation capabilities of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) emulsion compared with a liposomal counterpart but the cytotoxicity of DOTAP emulsions remained a challenge. The present study is aimed at formulating an emulsion capable of acting as a highly effective viral gene transfer vehicle with reduced cytotoxicity and to physicochemically characterize the structures of virus-emulsion complexes in comparison with virus–liposome complexes when the only difference between emulsions and liposomes was the presence or absence of inner oil core. The emulsion formulation was performed by 1) reducing the content of DOTAP while increasing the content of zwitterionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 2) optimizing the oil content. The complexation capability of formulated DOTAP:DMPC mixed emulsions was similar to those of emulsions containing DOTAP alone while displaying significantly lower cytotoxicity. The complexation capabilities of the DOTAP:DMPC mixed emulsion were serum-compatible and were monitored in a variety of cell types, whereas its liposomal counterpart was totally ineffective. Characterization by scanning electron microscopy, transmission electron microscopy, atomic force microscopy, and dynamic light scattering studies indicated that the optimized emulsions spontaneously surrounded the virus particles to generate emulsions that encapsulated the viral particles, whereas viral particles merely attached to the surfaces of the counterpart liposomes to form multiviral aggregates. Overall, these studies demonstrated that optimized DOTAP:DMPC mixed emulsions are potentially useful for adenoviral gene delivery due to less cytotoxicity and the unique ability to encapsulate the viral particle, highlighting the importance of nanoparticle formulation.

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Liposome-complexed adenoviral gene transfer in cancer cells expressing various levels of coxsackievirus and adenovirus receptor

Cited by 23 publications

References 26 publications

Gene transfer using liposome-complexed adenovirus seems to overcome limitations due to coxsackievirus and adenovirus receptor-deficiency of cancer cells, both in vitro and in vivo

Gene transfer using liposome-complexed adenovirus seems to overcome limitations due to coxsackievirus and adenovirus receptor-deficiency of cancer cells, both in vitro and in vivo

Suppression of ovarian cancer growth via systemic administration with liposome-encapsulated adenovirus-encoding endostatin

Optimization and physicochemical characterization of a cationic lipid-phosphatidylcholine mixed emulsion formulated as a highly efficient vehicle that facilitates adenoviral gene transfer

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