Liposome-cell interactions A study of the interactions of liposomes containing entrapped anti-cancer drugs with the EMT6, S49 and AE1 (transport-deficient) cell lines

Allen, Theresa M.; McAllister, Lowell; Mausolf, S.; Györffy, Erika

doi:10.1016/0005-2736(81)90080-8

Cited by 57 publications

(10 citation statements)

References 15 publications

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“…17,18 Liposomes are defined as bilayered lipid vesicles enclosing an aqueous volume. 19 Liposomes compose a significant vesicular carrier system for therapeutic effectiveness in terms of duration of action, decrease in dose frequency, and delivering drugs at a higher efficacy and lower toxicity. 20 Owing to the lipophilic nature of liposomes, they were considered an ideal brain-targeting drug-delivery system.…”

Section: Introductionmentioning

confidence: 99%

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer's disease

Ismail¹,

ElMeshad²,

Salem³

2013

IJN

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Background:To sustain the effect of rivastigmine, a hydrophilic cholinesterase inhibitor, nanobased formulations were prepared. The efficacy of the prepared rivastigmine liposomes (RLs) in comparison to rivastigmine solution (RS) was assessed in an aluminium chloride (AlCl 3 )-induced Alzheimer's model. Methods: Liposomes were prepared by lipid hydration (F1) and heating (F2) methods. Rats were treated with either RS or RLs (1 mg/kg/day) concomitantly with AlCl 3 (50 mg/kg/day). Results:The study showed that the F1 method produced smaller liposomes (67.51 ± 14.2 nm) than F2 (528.7 ± 15.5 nm), but both entrapped the same amount of the drug (92.1% ± 1.4%). After 6 hours, 74.2% ± 1.5% and 60.8% ± 2.3% of rivastigmine were released from F1 and F2, respectively. Both RLs and RS improved the deterioration of spatial memory induced by AlCl 3 , with RLs having a superior effect. Further biochemical measurements proved that RS and RLs were able to lower plasma C-reactive protein, homocysteine and asymmetric dimethylarginine levels. RS significantly attenuated acetylcholinesterase (AChE) activity, whereas Na + / K + -adenosine triphosphatase (ATPase) activity was enhanced compared to the AlCl 3 -treated animals; however, RLs succeeded in normalization of AChE and NaGene-expression profile showed that cotreatment with RS to AlCl 3 -treated rats succeeded in exerting significant decreases in BACE1, AChE, and IL1B gene expression. Normalization of the expression of the aforementioned genes was achieved by coadministration of RLs to AlCl 3 -treated rats. The profound therapeutic effect of RLs over RS was evidenced by nearly preventing amyloid plaque formation, as shown in the histopathological examination of rat brain. Conclusion: RLs could be a potential drug-delivery system for ameliorating Alzheimer's disease.

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Section: Introductionmentioning

confidence: 99%

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer's disease

Ismail¹,

ElMeshad²,

Salem³

2013

IJN

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“…First, the liposomes must deliver their contents efficiently to the cytoplasmic compartment. Second, the nonspecific toxicity, which may arise through the leakage of the cytotoxic agent from the liposome (7)(8)(9), must be minimized. The toxicitv of leaked drug could be minimized by using a drug that cannot enter cells but which would be toxic if delivered to the cytoplasm.…”

mentioning

confidence: 99%

Antibody-targeted liposomes: increase in specific toxicity of methotrexate-gamma-aspartate.

Heath¹,

Montgomery²,

Piper³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Liposomes conjugated with anti-H2Kk antibody associate with L929 murine fibroblasts in 6-to 20-fold greater amount than do nonspecific liposomes. The ability of methotrexate-y-aspartate to inhibit L929 growth is increased 10-fold when encapsulated in targeted liposomes but is decreased to 50% when encapsulated in liposomes with no specificity for the target cells. Ammonium chloride inhibits the effects of the encapsulated but not the free drug. Soluble antibody does not inhibit the efficacy of targeted vesicles, but empty targeted vesicles do inhibit the efficacy. The compound in both targeted and nontargeted vesicles has a minimal effect on BALB/c 3T6 fibroblasts. These results demonstrate the potential of antibody-targeted liposomes and the importance of selecting liposome-dependent cytotoxic agents.

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“…For this reason, molecules which are unable to penetrate cells are preferable because of the possibility of liposome leakage during incubation with the cells. Biologically active compounds such as anti-cancer drugs Allen et al, 1981), ribosome-inactivating proteins (Jansons and Panzner, 1983;Mclntosch and Heath, 1982), DNA (Fraley et al, 1981;Nicolau and Sene, 1982), and RNA (Lavelle et al, 1982) have been used to demonstrate liposome delivery into the cells. Because of our method of preparing vesicles, we used Hygromycin B, which is a convenient molecule that only inhibits protein synthesis in cell-free systems (Cabanas et al, 1978), or after permeation into cells in the presence of ionophores (Alonso and Carrasco, 1980).…”

Section: Discussionmentioning

confidence: 99%

“…evaluation requires the use of impermeant molecules. Thus, methotrexate and other cancer drugs (Allen et al, 1981), preclude any A.F. evaluation.…”

Section: Discussionmentioning

confidence: 99%

LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro.

Vidal¹,

Sainte‐Marie²,

Philippot³

et al. 1985

The EMBO Journal

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Communicated by P.DouzouWe describe a method for the covalent coupling of low-density lipoproteins (LDL) to the surface of small unilameliar vesicles, and the delivery of the liposome content to leukemic L2C Iymphocytes in vitro. We demonstrate the stability of the linkage between LDL and liposomes, the preservation of vesicle integrity and the affmity of the LDL for their specific receptors after the coupling reaction. Hygromycin B, an impermeant inhibitor of protein synthesis, was encapsulated in the targeted liposomes, and delivered into the cytoplasm of leukemic L2C lymphocytes by the LDL pathway, as demonstrated by the lethal effect on cells measured by 51chromium-release assay.

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Liposome-cell interactions A study of the interactions of liposomes containing entrapped anti-cancer drugs with the EMT6, S49 and AE1 (transport-deficient) cell lines

Cited by 57 publications

References 15 publications

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer's disease

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer's disease

Antibody-targeted liposomes: increase in specific toxicity of methotrexate-gamma-aspartate.

LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro.

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Liposome-cell interactions A study of the interactions of liposomes containing entrapped anti-cancer drugs with the EMT6, S49 and AE1 (transport-deficient) cell lines

Cited by 57 publications

References 15 publications

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer&#39;s disease

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer&#39;s disease

Antibody-targeted liposomes: increase in specific toxicity of methotrexate-gamma-aspartate.

LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro.

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Product

Resources

About

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer's disease

Potential therapeutic effect of nanobased formulation of rivastigmine on rat model of Alzheimer's disease