Liposome as a delivery system for the treatment of biofilm‐mediated infections

Wang, Yi

doi:10.1111/jam.15053

Cited by 53 publications

(55 citation statements)

References 105 publications

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“…Liposomes are reported to preferentially adsorb onto the biofilm surface and then penetrate into the EPS to eradicate bacterial growth. 15 , 16 Liposomes have also been demonstrated to be helpful for targeting hair follicles after the increase of flexibility. 17 Cationic surfactants can be coated on the nanoparticulate surface to kill the bacteria in biofilms by disintegrating the cell membrane through electrostatic and hydrophobic interactions.…”

Section: Introductionmentioning

confidence: 99%

Facile Biofilm Penetration of Cationic Liposomes Loaded with DNase I/Proteinase K to Eradicate Cutibacterium acnes for Treating Cutaneous and Catheter Infections

Fang¹,

Chou²,

Lin³

et al. 2021

IJN

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Background The biofilm produced by Cutibacterium acnes is a major infection threat for skin and implanted catheters. Nanoparticles provide a new approach to eradicate biofilms. The present study evaluated the capability of cationic liposomes loaded with DNase I (DNS) and proteinase K (PK) to remove preformed C. acnes biofilms. Methods DNS and PK were able to target and disassemble the biofilm by degrading extracellular polymer substances (EPS). Soyaethyl morpholinium ethosulfate (SME) was used to render a positive charge and enhance the antibacterial activity of the liposomes. Results The cationic liposomes containing enzymes yielded monodisperse nanovesicles ranging between 95 and 150 nm. The entrapment efficiency of the enzymes in the liposomes achieved a value of 67–83%. All liposomal formulations suppressed planktonic C. acnes growth at a minimum inhibitory concentration (MIC) equal to the free SME in the solution. The enzyme in the liposomal form inhibited biofilm growth much better than that in the free form, with the dual enzyme-loaded liposomes demonstrating the greatest inhibition of 54% based on a crystal violet assay. The biofilm-related virulence genes PA380 and PA1035 were downregulated by the combined enzymes in the liposomes but not the individual DNS or PK. Scanning electron microscopy (SEM) and confocal microscopy displayed reduced C. acnes aggregates and biofilm thickness by the liposomal system. The liposomes could penetrate through about 85% of the biofilm thickness. The in vitro pig skin permeation also showed a facile delivery of liposomes into the epidermis, deeper skin strata, and hair follicles. The liposomes exhibited potent activity to eliminate C. acnes colonization in mouse skin and catheters in vivo. The colony-forming units (CFUs) in the catheter treated with the liposomes were reduced by 2 logs compared to the untreated control. Conclusion The data suggested a safe application of the enzyme-loaded cationic liposomes as antibacterial and antibiofilm agents.

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Section: Introductionmentioning

confidence: 99%

Facile Biofilm Penetration of Cationic Liposomes Loaded with DNase I/Proteinase K to Eradicate Cutibacterium acnes for Treating Cutaneous and Catheter Infections

Fang¹,

Chou²,

Lin³

et al. 2021

IJN

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“…The encapsulation of vancomycin in fusogenic liposomes (which can fuse with the bacterial membrane) increases its bactericidal activity against biofilms of S. aureus 106 . Cationic liposomal capsules have been developed in order to penetrate the anionic matrix of S. aureus biofilms 107 . These drug-containing liposomes are more effective than the drug alone, being able to better slow down and inhibit bacterial biofilm growth.…”

Section: Contribution Of Nanotechnology Against Biofilmsmentioning

confidence: 99%

Recent advances in nanotechnology for eradicating bacterial biofilm

Sahli¹,

Moya²,

Lomas³

et al. 2022

Theranostics

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Microorganisms grouped together into spatially-organized communities called biofilms, are the cause of dramatic chronic infections in plants, animals and humans. In this review, the characteristics of biofilms and their interactions with antimicrobials are first described. Limitations of antibiotic treatments are discussed, and state-of-the-art alternative approaches based on the use of polymer, lipid, organic, inorganic and hybrid nanoparticles are presented, highlighting recent achievements in the application of nanomaterials to the field of theranostics for the eradication of biofilm. The aim of this review is to present a complete vision of nanobiotechnology-based approaches for eradicating bacterial biofilms and fighting antimicrobial tolerance.

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“…Successful delivery of vancomycin, gentamicin, and ampicillin and many other water-soluble antibiotics by mesoporous silica nanoparticle (MSN) has been reported [ 142 ]. Alternatively, hydrophilic antibiotics can be encapsulated by liposomes, a natural mimic of bacterial phospholipid bilayers, which can fuse with bacterial cell wall and allow deep penetration of antibacterial drugs to remove biofilm-mode pathogens [ 143 ]. When it comes to encapsulation of poorly water-soluble drugs, polymeric micellar structures are more suitable as they possess a hydrophobic core as drug reservoir.…”

Section: Microbial Resistance To Nanotechnologiesmentioning

confidence: 99%