Liposome accumulation in ischaemic intestine following experimental mesenteric occlusion

Abstract

Partial intestinal ischaemia was produced by ligation of selected primary laterals of the mesenteric artery in the rat. Both positively and negatively charged liposomes (multiply labelled with [99mTc]diethylenetriamine pentaacetic acid, [3H]methoxy-inulin, and [4-14C]-cholesterol), administered 24 h following ligation, were accumulated in ischemic (necrotic) intestine.

Liposomes in diagnostic radiology

“…99mTc-DTPA, as well as other chelates, accumulates in areas of ischaemia; therefore after the local release from liposomes the radiotracer remains associated with the lesion. A similar finding has been reported in ischaemic intestine (Palmer et al, 1981).…”

Radiolabelling of nanomaterials for medical imaging and therapy

“…Some of the earliest studies achieved this by simply encapsulating a radiometal complex with DTPA inside the liposomal core during formation of the liposomes (see Section 4.2.2). This was first done with 99m Tc, [275][276][277][278] and later with 111 In 279 and 159 Gd-DTPA, 280 as well as encapsulating the DOTA complex of 225 Ac. 281 One drawback of this method is the longer, more complicated radiosynthesis needed (especially relevant when using short-lived isotopes).…”

Nuclear imaging of liposomal drug delivery systems: A critical review of radiolabelling methods and applications in nanomedicine

“…Some of the earliest studies performing the radiolabelling of liposomes achieved this by simply encapsulating a radiometal complex with DTPA inside the liposomal core during formation of the liposomes. This was first done with 99m Tc [[96], [97], [98], [99]], and later 111 In [100] and 159 Gd-DTPA [101] – as well as with the therapeutic isotope 225 Ac by encapsulating the DOTA complex [102]. Alternatively, encapsulated drugs could themselves be labelled with radioiodine [56,[103], [104], [105], [106], [107], [108], [109]] or 18 F [110] before liposomal formulation and more recently liposomes were radiolabelled by being prepared in the presence of [ 18 F]FDG [[111], [112], [113], [114]].…”