Liposomal delivery systems for herbal extracts

Sogut, Oguz; Sezer, Ümran Aydemir; Sezer, Serdar

doi:10.1016/j.jddst.2020.102147

Cited by 34 publications

(37 citation statements)

References 129 publications

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“…Due to poor solubility and limited stability and bioavailability, the optimal exploitation of RES activities is dependent on a suitable carrier. Liposomes are well-established nanocarriers suitable for the delivery of polyphenols [14][15][16][17]. The phospholipid-based nanovesicles are biocompatible and allow entrapment of compounds of different molecular weights and lipophilicity [2].…”

Section: Introductionmentioning

confidence: 99%

Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginalTM Tissue Model

et al. 2022

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The natural polyphenol resveratrol (RES) has shown great potential as an antimicrobial, including against microbes associated with vaginal infections. To fully exploit the activities of RES, an all-natural ingredients formulation for RES delivery at vaginal site has been developed, namely liposomes loaded with RES, incorporated into a chitosan hydrogel as secondary vehicle. Although considered non-toxic and safe on their own, the compatibility of the final formulation must be evaluated for its biocompatibility and non-irritancy to the vaginal mucosa. As a preclinical safety assessment, the impact of RES formulation on the tissue viability, the effect on barrier function and cell monolayer integrity, and cytotoxicity were evaluated using the cell-based vaginal tissue model, the EpiVaginal™ tissue. RES liposomes-in-hydrogel formulations neither affected the mitochondrial activity, nor the integrity of the cell monolayer in RES concentration up to 60 µg/mL. Moreover, the barrier function was maintained to a greater extent by RES in formulation, emphasizing the benefits of the delivery system. Additionally, none of the tested formulations expressed an increase in lactate dehydrogenase activity compared to the non-treated tissues. The evaluation of the RES delivery system suggests that it is non-irritant and biocompatible with vaginal tissue in vitro in the RES concentrations considered as therapeutic.

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Section: Introductionmentioning

confidence: 99%

Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginalTM Tissue Model

et al. 2022

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“…One of the most important advantages of liposomes is that they can incorporate both lipophilic and hydrophilic compounds, making them suitable candidates for the encapsulation and delivery of plant extracts [32]. Yet, achieving the desired in vitro/in vivo effect is dependent on the quality attributes of the liposomal formulation, i.e., the encapsulated drug concentration, liposomal size, polydispersity index, or zeta potential [33,34].…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative and Antimicrobial Effects of Rosmarinus officinalis L. Loaded Liposomes

et al. 2022

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Rosmarinus officinalis L. is a species that is widely known for its culinary and medicinal uses. The purpose of the present study consisted of the evaluation of the antiproliferative and antimicrobial effects of R. officinalis-loaded liposomes (L-R). Characterization of the liposomes was performed by establishing specific parameters. The load of the obtained liposomes was analyzed using an LC-MS method, and antiproliferative assays evaluated the cell viability on a liver adenocarcinoma cell line and on a human hepatic stellate cell line. Antimicrobial assays were performed by agar–well diffusion and by broth microdilution assays. The obtained liposomes showed high encapsulation efficiency, suitable particle size, and good stability. High amounts of caffeic (81.07 ± 0.76), chlorogenic (14.10 ± 0.12), carnosic (20.03 ± 0.16), rosmarinic (39.81 ± 0.35), and ellagic (880.02 ± 0.14) acids were found in their composition, together with other polyphenols. Viability and apoptosis assays showed an intense effect on the cancerous cell line and a totally different pattern on the normal cells, indicating a selective toxicity towards the cancerous ones and an anti-proliferative mechanism. Antimicrobial potential was noticed against all tested bacteria, with a better efficacy towards Gram-positive species. These results further confirm the biological activities of R. officinalis leaf extract, and proposes and characterizes novel delivery systems for their encapsulation, enhancing the biological activities of polyphenols, and overcoming their limitations.

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“…Indeed, it was previously demonstrated that the beneficial properties of each phytocomplex or phytochemical can be maximized by selecting the most suitable delivery systems [ 16 ]. Phospholipid vesicles were chosen because of their versatility and their ability to simultaneously load both hydrophilic and lipophilic compounds, thus being ideal carriers for the incorporation of plant-derived phytocomplexes, containing different bioactive molecules [ 46 ]. Hyalurosomes were selected due to their optimal performance and effectiveness on skin lesions, and they were further enriched by using Tween 80, a surfactant, which can act as an edge activator, and glycerol, which has an emollient and moisturizing effect on the skin [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Formulation and In Vitro Efficacy Assessment of Teucrium marum Extract Loading Hyalurosomes Enriched with Tween 80 and Glycerol

et al. 2022

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The extract of Teucrium marum L. (Lamiaceae) was obtained using the aerial parts of the plant, by means of a maceration process. Verbascoside, caffeic acids derivatives and flavonols were the main components contained in the extract as detected using high-performance liquid chromatography coupled with diode array detector (HPLC–DAD) as an analytical method. The extract was successfully incorporated into hyalurosomes, which were further enriched by adding a water cosolvent (glycerol) and a surfactant (Tween 80), thus obtaining glycerohyalurosomes. Liposomes, transfersomes and glycerosomes were prepared as well and used as comparisons. All vesicles were small, as the mean diameter was never higher than ~115 nm, thus ideal for topical application and stable on storage, probably thanks to the highly negative surface charge of the vesicles (~−33 mV). The cryo-TEM images confirmed the formation of close-packed, oligolamellar and multicompartment hyalurosomes and glycerohyalurosomes in which around 95% of the used extract was retained, confirming their ability to simultaneously load a wide range of molecules having different chemical natures. Moreover, the extract, when loaded in hyalurosomes and glycerohyalurosomes was able to counteract the damages induced in the fibroblasts by hydrogen peroxide to a better extent (viability~110%) than that loaded in the other vesicles (viability~100%), and effectively promoted their proliferation and migration ensuring the healing of the wound performed in a cell monolayer (scratch assay) during 48 h of experiment. Overall in vitro results confirmed the potential of glycerohyalurosomes as delivery systems for T. marum extract for the treatment of skin lesions connected with oxidative stress.

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Liposomal delivery systems for herbal extracts

Cited by 34 publications

References 129 publications

Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginalTM Tissue Model

Toxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginalTM Tissue Model

Antiproliferative and Antimicrobial Effects of Rosmarinus officinalis L. Loaded Liposomes

Formulation and In Vitro Efficacy Assessment of Teucrium marum Extract Loading Hyalurosomes Enriched with Tween 80 and Glycerol

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