Liposomal adhesion <i>via</i> electrostatic interactions and osmotic deflation increase membrane tension and lipid diffusion coefficient

Oda, Atsushi; Watanabe, Chiho; Aoki, Natsumi; Yanagisawa, Miho

doi:10.1039/d0sm00416b

Cited by 14 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There has been a recent growing interest in assembling liposomes into connected “multicellular”-like MC structures to expand their functionalities. − Reported strategies to achieve MC lipid-based structures have included microfluidic assemblies, spontaneous assemblies, electrostatic interactions, , manual manipulation by optical traps, and through emulsion phase transfer by allowing lipid-stabilized emulsion droplets to self-concentrate by settling at the interphase before transfer . Separately, MC structures have also been formed as “droplet networks”, whereby lipid stabilized aqueous droplets are connected together by hemi-fusion inside an external oil phase by simple proximity and without coalescence.…”

Section: Introductionmentioning

confidence: 99%

Scalable Synthesis of Planar Macroscopic Lipid-Based Multi-Compartment Structures

et al. 2023

View full text Add to dashboard Cite

As life evolved, the path from simple single cell organisms to multicellular enabled increasingly complex functionalities. The spatial separation of reactions at the micron scale achieved by cellular structures allowed diverse and scalable implementation in biomolecular systems. Mimicking such spatially separated domains in a scalable approach could open a route to creating synthetic cell-like structured systems. Here, we report a facile and scalable method to create multicellular-like, multi-compartment (MC) structures. Aqueous droplet-based compartments ranging from 50 to 400 μm were stabilized and connected together by hydrophobic layers composed of phospholipids and an emulsifier. Planar centimeter-scale MC structures were formed by droplet deposition on a water interface. Further, the resulting macroscopic shapes were shown to be achieved by spatially controlled deposition. To demonstrate configurability and potential versatility, MC assemblies of both homogeneous and mixed compartment types were shown. Notably, magnetically heterogeneous systems were achieved by the inclusion of magnetic nanoparticles in defined sections. Such structures demonstrated actuated motion with structurally imparted directionality. These novel and functionalized structures exemplify a route toward future applications including compartmentally assembled “multicellular” molecular robots.

show abstract

Section: Introductionmentioning

confidence: 99%

Scalable Synthesis of Planar Macroscopic Lipid-Based Multi-Compartment Structures

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The droplet transfer method, which is also known as the inverted emulsion method, has been widely used for the formation of giant vesicles (GVs) with a diameter of tens of micrometers ( Pautot et al, 2003 ; Walde et al, 2010 ). GVs have been applied as a model cell membrane for the investigation of the physical properties of lipid membranes in the field of soft-matter physics ( Jimbo et al, 2016 ; Oda et al, 2020 ; Lowe et al, 2022 ), and for the construction of artificial cells in the field of synthetic biology ( Lu et al, 2021 ). Although several methods have been developed to form GVs, such as electro formation ( Angelova and Dimitrov, 1986 ) or film hydration ( Reeves and Dowben, 1969 ; Tsumoto et al, 2009 ), the droplet transfer method ( Moga et al, 2019 ) has advantages in the generation of single lamellar membrane vesicles and its application to biochemical experiments requiring physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction

Shimane

Kuruma

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Giant vesicles have been widely used for the bottom-up construction of artificial (or synthetic) cells and the physicochemical analysis of lipid membranes. Although methods for the formation of giant vesicles and the encapsulation of molecules within them have been established, a standardized protocol has not been shared among researchers including non-experts. Here we proposed a rapid and facile protocol that allows the formation of giant vesicles within 30 min. The quality of the giant vesicles encapsulating a cell-free protein expression system was comparable to that of the ones formed using a conventional method, in terms of the synthesis of both soluble and membrane proteins. We also performed protein synthesis in artificial cells using a lyophilized cell-free mixture and showed an equivalent level of protein synthesis. Our method could become a standard method for giant vesicle formation suited for artificial cell research.

show abstract

“…[11,12] Just as organisms in nature have evolved from unicellular to multicellular, research on multicompartmentalization of artificial cells has received much attention. [13,14] There have been reports on the adhesion of postformed liposomes, [15,16] multicompartment emulsions containing drugs by centrifugal sedimentation, [17] 3D-printing injection molding of water-in-oil emulsion droplets, [18] and re-exposure of the structure to water to form lipid bilayers. [19] These methods typically require sophisticated machinery such as specialized microfluidic channels, modified 3D printers, etc.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and Driven Growth of Multicellular Lipid Compartments to Millimeter Size from Porous Polymer Structures**

et al. 2022

View full text Add to dashboard Cite

This report describes a method to obtain multicellular shaped compartments made by lipids growing from a sponge-like porous structure. Each compartment is several tens of micrometers in diameter and separated by membranes comprised of phospholipid and amphipathic molecules. The multi-compartment structure spontaneously grew to a millimeter scale, driven by an ionic concentration difference between the interior and exterior environments of the sponge. These compartments can also easily incorporate hydrophilic species as a well as smaller materials such as liposomes. Additionally, we showed that mechanical squeezing of the sponge was also effective in producing multicellular bodies. These simple methods to obtain large-scale multicellular compartment of lipid membrane will help future designs and trials of chemical communications on artificial cells.

show abstract

Liposomal adhesion via electrostatic interactions and osmotic deflation increase membrane tension and lipid diffusion coefficient

Abstract: Liposome–liposome adhesion by electrostatic interactions and osmotic contraction increase membrane tension and the lipid diffusion coefficient compared to isolated liposomes.

Cited by 14 publications

References 36 publications

Scalable Synthesis of Planar Macroscopic Lipid-Based Multi-Compartment Structures

Scalable Synthesis of Planar Macroscopic Lipid-Based Multi-Compartment Structures

Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction

Spontaneous and Driven Growth of Multicellular Lipid Compartments to Millimeter Size from Porous Polymer Structures**

Contact Info

Product

Resources

About