Lipoproteins from Clostridium perfringens and their protective efficacy in mouse model

Dwivedi, Pratistha; Alam, Syed Imteyaz; Kumar, Om; Kumar, Rohit

doi:10.1016/j.meegid.2015.05.029

Cited by 8 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Clostridium perfringens , the causative agent of gas gangrene, uses lipoproteins for host colonization and virulence. Three lipoproteins were assessed as potential immunization targets, and recombinant protein vaccination with PolDe induced a protective mixed Th1 and Th2 response . Also, the Tul4 lipoprotein from Francisella tularensis has induced protective effects upon vaccination through an adenoviral vector or as recombinant protein ligated onto the surface of a tobacco mosaic virus .…”

Section: Lipoprotein‐based Immunizationmentioning

confidence: 99%

Mycobacterium tuberculosis lipoproteins in virulence and immunity – fighting with a double‐edged sword

Becker

Sander

2016

FEBS Letters

View full text Add to dashboard Cite

Bacterial lipoproteins are secreted membrane‐anchored proteins characterized by a lipobox motif. This lipobox motif directs post‐translational modifications at the conserved cysteine through the consecutive action of three enzymes: Lgt, LspA and Lnt, which results in di‐ or triacylated forms. Lipoproteins are abundant in all bacteria including Mycobacterium tuberculosis and often involved in virulence and immunoregulatory processes. On the one hand, disruption of the biosynthesis pathway of lipoproteins leads to attenuation of M. tuberculosis in vivo, and mycobacteria deficient for certain lipoproteins have been assessed as attenuated live vaccine candidates. On the other hand, several mycobacterial lipoproteins form immunodominant antigens which promote an immune response. Some of these have been explored in DNA or subunit vaccination approaches against tuberculosis. The immune recognition of specific lipoproteins, however, might also benefit long‐term survival of M. tuberculosis through immune modulation, while others induce protective responses. Exploiting lipoproteins as vaccines is thus a complex matter which requires deliberative investigation. The dual role of lipoproteins in the immunity to and pathogenicity of mycobacteria is discussed here.

show abstract

Section: Lipoprotein‐based Immunizationmentioning

confidence: 99%

Mycobacterium tuberculosis lipoproteins in virulence and immunity – fighting with a double‐edged sword

Becker

Sander

2016

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…From the SDS PAGE of recovered proteins, eight most abundant protein bands for bottle brush exposure and five most abundant protein bands for glass and sand exposure were excised and subjected to in-gel digestion and subsequent identification. The cloning and expression strategies for cOTC and PolDe have been described previously, , while FBA was expressed using the same procedure as for PolDe. Expression vector pET 28 “a” (Novagen, Germany) and E. coli strain BL21-DE3 (Novagen, Germany) were used for FBA and PolDe, while pQE30-UA vector (Qiagen, Germany) and E. coli M15 cells were used for the expression of recombinant cOTC.…”

Section: Methodsmentioning

confidence: 99%

Postexposure Recovery and Analysis of Biological Agent in a Simulated Biothreat Scenario Using Tandem Mass Spectrometry

et al. 2017

Self Cite

View full text Add to dashboard Cite

Some pathogens and toxins have the potential to be used as weapons of mass destruction and instigate population-based fear. Rapid, sensitive, and unambiguous identification of biothreat agents is of paramount importance for confirmation of the event and to mitigate the direct and indirect damages to public health and resources. Although there are several potential dissemination scenarios to describe an attack with a biological weapon, artificially generated bioaerosol is of the greatest concern from a bioterrorism or warfare perspective, potentially capable of causing mass destruction to a civilian or military population by inhalation of toxic bioaerosol. The present investigation proposes methodologies for recovery of biological agent followed by an off-site unambiguous detection using tandem mass spectrometry, in a postattack situation. We envisaged a biothreat scenario wherein the polydispersed bioaerosol is disseminated in bulk over any geographical setting. The larger particles (>5 μm in diameter) of bioaerosol settle and bind to various surfaces depending on the geographical setting. Recovery of agent was optimized from foliage, sand, and glass in a simulated biothreat scenario using bovine serum albumin (BSA). The recovered agents were shown to be amenable to detection by a downstream tandem MS analysis. Applicability of the proposed methodology was demonstrated in validation experiments for the recovery and detection of toxin and bacterial agents. The use of cleaner matrices (foliage, exposed smooth surfaces, sand) is recommended for retrospective verification of agent in a biothreat scenario.

show abstract

“…Indirect ELISA was used to quantify the antibody response to the recombinant hypothetical protein CPF_2918 as described earlier (Dwivedi et al, 2015). Briefly, the microtitration plates serum with an OD greater than the mean OD of the preimmune serum (1:100 dilution) in the same group was considered the endpoint titer.…”

Section: Antibody Response To Recombinant Cpf_2918mentioning

confidence: 99%

“…In our previous proteomic elucidation of extracellular milieu or surface fractions of C. perfringens, several abundant proteins were identified as hypothetical proteins (Alam et al, 2009;Sengupta et al, 2010;Dwivedi et al, 2015). Some of these hypothetical proteins were identified as differentially expressed in the extractable proteome of highly virulent C. perfringens type A strain; arguably, having putative role in the host specificity and pathogenesis of C. perfringens (Dwivedi et al, 2015). These studies clearly demonstrated presence of at least eighteen hypothetical proteins A C C E P T E D M A N U S C R I P T…”

Section: Introductionmentioning

confidence: 99%

“…These proteins were either directly identified from C. perfringens ATCC13124, the clinical gas gangrene strain, or as a close homolog in other virulent gas gangrene causing strains isolated in our laboratory(Sengupta et al, 2010;Dwivedi et al, 2015). In the present study we have explored the putative role of these hypothetical proteins using bioinformatics tools and also evaluated the potential of these HPs as putative candidates for prophylaxis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Putative function of hypothetical proteins expressed by Clostridium perfringens type A strains and their protective efficacy in mouse model

Alam

Dwivedi

2016

Infection, Genetics and Evolution

Self Cite

View full text Add to dashboard Cite

Lipoproteins from Clostridium perfringens and their protective efficacy in mouse model

Cited by 8 publications

References 45 publications

Mycobacterium tuberculosis lipoproteins in virulence and immunity – fighting with a double‐edged sword

Mycobacterium tuberculosis lipoproteins in virulence and immunity – fighting with a double‐edged sword

Postexposure Recovery and Analysis of Biological Agent in a Simulated Biothreat Scenario Using Tandem Mass Spectrometry

Putative function of hypothetical proteins expressed by Clostridium perfringens type A strains and their protective efficacy in mouse model

Contact Info

Product

Resources

About