Lipoprotéines plasmatiques du veau préruminant

Bauchart, Dominique; Levieux, Didier; MEYER, Marinette; LEVIEUX, Monique

doi:10.1051/rnd:19850214

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…During the postprandial period, a large increase in the plasma concentrations of calf chylomicrons and VLDL was noted from 7 h after meal intake. Furthermore, the fatty acid composition of triacylglycerols of these particles was then representative of the dietary fatty acid composition (Bauchart & Levieux, 1985). These results provided evidence that in the calf fed on curdled milk substitute peak lipid absorption was 5-7 h later than that in conventional singlestomached animals.…”

Section: Lipid Digestion In Ruminantssupporting

confidence: 53%

“…Utilizing electromagnetic blood-flow measurement, we have shown that in calves fed on a milk diet which curdles in the abomasum, portal blood flow rapidly increases 1 h after meal intake, probably in response to the intestinal absorption of glucose (Durand et al 1988). Hyperglycaemia noted 46-90 min after intake increased plasma insulin which stimulated the degradation of plasma triacylglycerols by lipoprotein lipase (EC 3.1.1.34; LPL; Bauchart & Levieux, 1985). This was followed by the clot formation in the abomasum decreasing portal blood flow for the next 4 h due to the drop in mesenteric blood flow (which constitutes 65% of the portal blood flow) following a lack of nutrient absorption during this period.…”

Section: Lipid Digestion In Ruminantsmentioning

confidence: 99%

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Lipid absorption and hepatic metabolism in ruminants

1996

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L'absorption des lipides et le mCtabolisme hCpatique des ruminants R E S U M ELes matikres grasses sont ajoutCes aux rations des ruminants pour ameliorer l'efficacite d'utilisation de 1'Cnergie B des fins de production. L'intestin gr&le des ruminants posskde une forte capacitC d'absorption des acides gras alimentaires saturCs par l'action des hydrogenases bactkriennes du rumen. La synthkse et la secretion des lipoproteines riches en triacylglycerols (chylomicrons, VLDL) par l'intestin grCle varie avec le degri d'insaturation des chaines grasses alimentaires. Le lent processus d'absorption des acides gras chez le veau prkruminant favorise la sCcrCtion des lipoprotkines riches en triacylglycerols par la voie de la veine porte. La fraction protkique de ces particules lipoprot6iques est dominCe, chez le bovin, par l'apoprotkine B48, forme tronquee de l'apo B resultant de la modification unique post-transcriptionnelle de I'ARN messager de l'apo B. Le metabolisme hepatique des lipides des ruminants prCsente des particularites favorisant la formation de foie gras et le dCveloppement d'une cCtoacidose nuisible B la sante et aux capacites de reproduction des animaux. Le foie possede une forte capacite d'estkrification et de stockage des acides gras exogknes sous forme de triacylglycerols ou d'oxydation de ces acides gras sous forme de corps cktoniques. Les faibles capacitCs de synthkse de l'apo BlOO et de mobilisation des triacylglycCrols stock& dans le cytoplasme limitent fortement la secrktion hkpatique des triacylglycerols sous forme de VLDL. Chez les femelles en debut de lactation, le diveloppement de l'infiltration lipidique du foie n'est pas rCduit par la supplementation lipidique des rations mais plut8t par l'apport alimentaire d'acides amines limitants (mkthionine, lysine). La modulation de I'expression du gkne hkpatique de l'apo B au cours du cycle gestation-lactation explique, en partie, le developpement de l'infiltration graisseuse observee en debut de lactation.With the exception of milk substitutes in young preruminant animals, the diets of cattle contain little or no added fat. However, in the last two decades, there has been increasing interest in adding fat to the diets of growing and lactating cattle in order to increase efficiency of energy utilization for animal performance. Justifications for this approach, recently summarized by Palmquist (1988Palmquist ( , 1994 and Clinquart et al. (1995), include (1) increased energy density of the diets, (2) increased availability of high-quality fats from industry, (3) improved quality of the carcass of growing-fattening cattle, (4) improved dietetic quality of fat in milk and in muscle tissues, ( 5 ) improved lactation and reproductive performance and metabolic efficiency of lactating cattle. In order to protect https://www.cambridge.org/core/terms. https://doi

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Section: Lipid Digestion In Ruminantssupporting

confidence: 53%

Section: Lipid Digestion In Ruminantsmentioning

confidence: 99%

Lipid absorption and hepatic metabolism in ruminants

1996

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“…In all three groups concentrations of p-carotene in VLDL and LDL are higher than in cows and bulls. This is due to a higher concentration of these fractions in suckling calves (BAUCHART and LEVIEUX 1985) and in cows during the dry period, where RAPHAEL et al (1973) found a two-to-three fold increase in VLDL. The existence of chylomicrones in suckling calves contrary to mature cattle has already been reported by CHAPLIN and PALMQUIST (1971).…”

Section: Discussionmentioning

confidence: 97%

Transport of β‐carotene by the serum lipoproteins in cattle

Schweigert

Rambeck

Zücker

1987

Animal Physiology Nutrition

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Zusammenfassung Transport von β‐Carotin durch die Serumlipoproteine des Rindes Die Untersuchung über den Transport von β‐Carotin durch die Serumlipoproteinfraktionen bei Kühen, Bullen, Jungrindern und Saugkälbern zeigt, daß alle drei Lipoproteinfraktionen β‐Carotin transportieren. Mit 82% der β‐Carotin‐Konzentration des Serum transportiert bei laktierenden Kühen und Bullen die, high‐density “Lipoproteinfraktion (HDL) den größten Anteil, gefolgt von den, low‐density” Lipoproteinen (LDL) mit 12% und den, very low‐density Lipoproteinen (VLDL) mit 0.3%. Weder die Höhe der β‐Carotin‐Konzentration im Serum noch die Menge des über die Milch ausgeschiedenen β‐Carotins beeinflußte die Verteilung. Der Anstieg des Anteils von β‐Carotin in der VLDL‐ und LDL‐Fraktion, wie er bei trokkenstehenden Kühen, Jungrindern und Saugkälbern festzustellen ist, beruht auf einer Zunahme dieser Fraktionen gegenüber der HDL‐Fraktion.

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“…Moreover, the higher and faster cellular incorporation from Pyr-met-Chol-labelled purified LDL than from Pyr-met-Chol-labelled purified HDL ( Fig 2B ) is also indicative of an interaction with the corresponding specific receptors, and this is consistent with the reported overexpression of LDL receptors in the PC-3 cells [ 21 , 35 ]. However, when PC-3 cells are incubated in the presence of total serum, the relative contributions of LDL- and HDL-mediated incorporation routes are expected to be fairly comparable since HDL are present in foetal calf serum in somehow higher (2–3 fold) amounts than LDL [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Specific Cellular Incorporation of a Pyrene-Labelled Cholesterol: Lipoprotein-Mediated Delivery toward Ordered Intracellular Membranes

Gaibelet¹,

Allart²,

Tercé³

et al. 2015

PLoS ONE

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In the aim of testing tools for tracing cell trafficking of exogenous cholesterol, two fluorescent derivatives of cholesterol, 22-nitrobenzoxadiazole-cholesterol (NBD-Chol) and 21-methylpyrenyl-cholesterol (Pyr-met-Chol), with distinctive chemico-physical characteristics, have been compared for their cell incorporation properties, using two cell models differently handling cholesterol, with two incorporation routes. In the Caco-2 cell model, the cholesterol probes were delivered in bile salt micelles, as a model of intestinal absorption. The two probes displayed contrasting behaviors for cell uptake characteristics, cell staining, and efflux kinetics. In particular, Pyr-met-Chol cell incorporation involved SR-BI, while that of NBD-Chol appeared purely passive. In the PC-3 cell model, which overexpresses lipoprotein receptors, the cholesterol probes were delivered via the serum components, as a model of systemic delivery. We showed that Pyr-met-Chol-labelled purified LDL or HDL were able to specifically deliver Pyr-met-Chol to the PC-3 cells, while NBD-Chol incorporation was independent of lipoproteins. Observations by fluorescence microscopy evidenced that, while NBD-Chol readily stained the cytosolic lipid droplets, Pyr-met-Chol labelling led to the intense staining of intracellular structures of membranous nature, in agreement with the absence of detectable esterification of Pyr-met-Chol. A 48 h incubation of PC-3 cells with either Pyr-met-Chol-labelled LDL or HDL gave same staining patterns, mainly colocalizing with Lamp1, caveolin-1 and CD63. These data indicated convergent trafficking downwards their respective receptors, LDL-R and SR-BI, toward the cholesterol-rich internal membrane compartments, late endosomes and multivesicular bodies. Interestingly, Pyr-met-Chol staining of these structures exhibited a high excimer fluorescence emission, revealing their ordered membrane environment, and indicating that Pyr-met-Chol behaves as a fair cholesterol tracer regarding its preferential incorporation into cholesterol-rich domains. We conclude that, while NBD-Chol is a valuable marker of cholesterol esterification, Pyr-met-Chol is a reliable new lipoprotein fluorescent marker which allows to probe specific intracellular trafficking of cholesterol-rich membranes.

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Lipoprotéines plasmatiques du veau préruminant

Abstract: Summary. Plasma Introduction.

Cited by 9 publications

References 13 publications

Lipid absorption and hepatic metabolism in ruminants

Lipid absorption and hepatic metabolism in ruminants

Transport of β‐carotene by the serum lipoproteins in cattle

Specific Cellular Incorporation of a Pyrene-Labelled Cholesterol: Lipoprotein-Mediated Delivery toward Ordered Intracellular Membranes

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