Lipoprotein pre-staining: Comparison of nitroblue tetrazolium and Sudan Black B

Green, J.

doi:10.1016/0009-8981(76)90228-x

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…The pre-staining and electrophoretic techniques have been described (Green, 1976). With this procedure, chylomicrons remain in the top few millimetres of the gel; they are poorly stained if at all, by Sudan Black; VLDL appear as a single, or rarely as a double, diffuse band in a 3.5-4 g/100 ml gradient.…”

Section: Analytical Procedures and Preliminary Studiesmentioning

confidence: 99%

Molecular Exclusion Electrophoresis of Human Serum Lipoproteins: Patterns in Control and Ischaemic Heart-Disease Populations

Green

Carney

1977

Clinical Science

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1. Electrophoresis of pre-stained lipoproteins on acrylamide-gel gradients has been carried out on serum from populations of control subjects and patients with ischaemic heart disease. The technique resolves components intermediate in position and, by inference, in size, between very-low-density and low-density lipoproteins. 2. These central band components were found in 37% of a control population but the incidence varied with age and sex, being lowest in young males and highest in elderly males. 3. The incidence of central band components in patients with ischaemic heart disease was 64% (males) and 71% (females), and the difference between these figures and those for matched control subjects was highly significant. The intensity of central band components in the group with ischaemic heart disease was significantly greater than in the control group. 4. The presence and intensity of central bands show positive correlation with serum cholesterol and triglyceride values, but many patients showing the phenomenon have normal lipid values. Of patients with ischaemic heart disease 31% showed central band components and had normal lipid values.

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Section: Analytical Procedures and Preliminary Studiesmentioning

confidence: 99%

Molecular Exclusion Electrophoresis of Human Serum Lipoproteins: Patterns in Control and Ischaemic Heart-Disease Populations

Green

Carney

1977

Clinical Science

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“…This technique, however, uses the discrepancy (sic) between the appearance of low density lipoprotein (LDL) or ,8 lipoprotein on polyacrylamide and agarose gel electrophoresis as a means of diagnosis (Masket et al, 1973). Molecular exclusion electrophoresis on acrylamide gradients (Green, 1976) should display the very low density (VLDL)-LDL spectrum in order of molecular size.…”

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confidence: 99%

“…Material and methods ELECTROPHORESIS Separation of serum lipoproteins prestained with Sudan Black B was performed on acrylamide gradients, as described by Green (1976), with the following modifications. 0-13 ml of 3M TRIS-HCl, pH 8-9, was added to 4 ml of the propane-1, 2-diol Sudan Black solution to minimise early cathodal migration.…”

mentioning

confidence: 99%