Lipoprotein lipase hydrolysis of retinyl ester. Possible implications for retinoid uptake by cells

Blaner, William S.; Obunike, Joseph C.; Kurlandsky, Sara; Al-Haideri, Maysoon; Piantedosi, Roseann; Deckelbaum, Richard J.; Goldberg, Ira J.

doi:10.1016/s0021-9258(19)89425-5

Cited by 121 publications

(38 citation statements)

References 40 publications

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“…Briefly, [ 14 C]hexadecyl mesylate was synthesized from 100 Ci [1-14 C]palmitic acid (50 mCi/mmol, Du-Pont NEN) and the labeled mesylate was reacted with 100 mg unlabeled retinol (which had been purified immediately prior to use on a column of 10% deactivated alumina), to yield retinyl [ 14 C]hexadecyl ether. The newly synthesized radiolabeled retinoids were purified on columns of 5% deactivated alumina followed by chromatography on a normal phase HPLC system (14).…”

Section: Preparation Of Intralipid Emulsions Doubly Labeled With [ 3 H]retinyl Palmitate and Retinyl [ 14 C]hexadecyl Ethermentioning

confidence: 99%

“…Therefore, neither the process of sonication nor the incorporation of the tracer doses of the radiolabeled retinoids into the emulsion had influenced the composition of the emulsion. Moreover, as greater than 90% of total 3 H and 14 C cpm placed into the capillary tube were recovered in the top fraction after centrifugation and as the ratios of 3 H cpm, 14 C cpm, triglyceride, and phospholipid remained constant, the labeled retinoids must have been incorporated homogeneously into the Intralipid emulsion.…”

Section: Preparation Of Intralipid Emulsions Doubly Labeled With [ 3 H]retinyl Palmitate and Retinyl [ 14 C]hexadecyl Ethermentioning

confidence: 99%

“…After vigorous mixing, centrifugation to separate phases and removal of the lower lipid containing chloroform phase, the total lipid extracts were allowed to evaporate in a standard laboratory hood and the resulting lipid film was redissolved in 20 mL Hydrofluor liquid scintillation counting solution (National Diagnostics, Atlanta, Georgia). 3 H and 14 C cpm were assayed simultaneously in a Beckman LS 1800 Liquid Scintillation Counter. The spillover of 14 C cpm into the 3 H channel was 4.8% and the spillover of 3 H cpm into the 14 C channel was 7.5%.…”

Section: Liquid Scintillation Countingmentioning

confidence: 99%

“…Thus, LpL concentration influenced cellular uptake of retinyl esters from either chylomicrons and/or their remnants. Based on these observations, we hypothesized that differences in the level of LpL expression and/or LpL activity influence tissue uptake of chylomicron retinyl esters by extrahepatic tissues in vivo (14). The present study explores this possibility.…”

mentioning

confidence: 91%

“…We (14) and others (15) have reported that LpL can catalyze hydrolysis of chylomicron retinyl esters after most of the triglyceride present in the chylomicrons has first been hydrolyzed (14). We further demonstrated that the amount of total retinol (retinol ϩ retinyl ester) taken up by murine BFC-1 ␤ adipocyte cultures from retinyl ester-containing chylomicrons was greatly increased by addition of exogeneous LpL to the cultured cells (14). Thus, LpL concentration influenced cellular uptake of retinyl esters from either chylomicrons and/or their remnants.…”

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confidence: 99%

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Lipoprotein lipase expression level influences tissue clearance of chylomicron retinyl ester

Bennekum

Kako

Weinstock

et al. 1999

Journal of Lipid Research

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Approximately 25% of postprandial retinoid is cleared from the circulation by extrahepatic tissues. Little is known about physiologic factors important to this uptake. We hypothesized that lipoprotein lipase (LpL) contributes to extrahepatic clearance of chylomicron vitamin A. To investigate this, [ 3 H]retinyl ester-containing rat mesenteric chylomicrons were injected intravenously into induced mutant mice and nutritionally manipulated rats. The tissue sites of uptake of 3 H label by wild type mice and LpL-null mice overexpressing human LpL in muscle indicate that LpL expression does influence accumulation of chylomicron retinoid. Skeletal muscle from mice overexpressing human LpL accumulated 1.7-to 2.4-fold more 3 H label than wild type. Moreover, heart tissue from mice overexpresssing human LpL, but lacking mouse LpL, accumulated less than half of the 3 H-label taken up by wild type heart. Fasting and heparin injection, two factors that increase LpL activity in skeletal muscle, increased uptake of chylomicron [ 3 H] retinoid by rat skeletal muscle. Using [ 3 H]retinyl palmitate and its non-hydrolyzable analog retinyl [ 14 C]hexadecyl ether incorporated into Intralipid emulsions, the importance of retinyl ester hydrolysis in this process was assessed. We observed that 3 H label was taken up to a greater extent than 14 C label by rat skeletal muscle, suggesting that retinoid uptake requires hydrolysis. In summary, for each of our experiments, the level of lipoprotein lipase expression in skeletal muscle, heart, and/or adipose tissue influenced the amount of [ 3 H]retinoid taken up from chylomicrons and/ or their remnants.-

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Section: Preparation Of Intralipid Emulsions Doubly Labeled With [ 3 H]retinyl Palmitate and Retinyl [ 14 C]hexadecyl Ethermentioning

confidence: 99%

Section: Preparation Of Intralipid Emulsions Doubly Labeled With [ 3 H]retinyl Palmitate and Retinyl [ 14 C]hexadecyl Ethermentioning

confidence: 99%

Section: Liquid Scintillation Countingmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 99%

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Lipoprotein lipase expression level influences tissue clearance of chylomicron retinyl ester

Bennekum

Kako

Weinstock

et al. 1999

Journal of Lipid Research

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Vitamin A Metabolism, Storage and Tissue Delivery Mechanisms

Blaner

2015

The Retinoids

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Retinoids: Potent regulators of metabolism

Brun¹,

Yang²,

Lee³

et al. 2012

BioFactors

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Retinoids (vitamin A and its analogs) are highly potent regulators of cell differentiation, cell proliferation, and apoptosis. Because of these activities, retinoids have been most extensively studied in the contexts of embryonic development and of proliferative diseases, especially cancer and skin disease. Recently, there has been considerable new research interest focused on gaining understanding of the roles that retinoids and/or retinoid-related proteins may have in the development of metabolic diseases, primarily obesity, diabetes, and dyslipidemia. This review will summarize recent advances that have been made in these areas, focusing on the role of retinoids in modulating adipogenesis, the roles of retinoids and retinoid-related proteins as signaling molecules linking obesity with the development of type II diabetes, the roles of retinoids in pancreatic β-cell biology/insulin secretion, and the actions of retinoids in hepatic steatosis.

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Lipoprotein lipase hydrolysis of retinyl ester. Possible implications for retinoid uptake by cells

Cited by 121 publications

References 40 publications

Lipoprotein lipase expression level influences tissue clearance of chylomicron retinyl ester

Lipoprotein lipase expression level influences tissue clearance of chylomicron retinyl ester

Vitamin A Metabolism, Storage and Tissue Delivery Mechanisms

Retinoids: Potent regulators of metabolism

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