Lipoprotein lipase does not increase significantly in the postprandial plasma

Ishiyama, Nobuyoshi; Sakamaki, Kouji; Shimomura, Y; Kotani, Kazuhiko; Tsuzaki, Kokoro; Sakane, Naoki; Miyashita, Kazuya; I, Fukamachi; Kobayashi, Junji; Stanhope, Kimber L.; Havel, Peter J.; Kamachi, Keiko; Tanaka, Akira; Tokita, Yoshiharu; Machida, Tetsuo; Murakami, Masami; Nakajima, Katsuyuki

doi:10.1016/j.cca.2016.11.035

Cited by 12 publications

(8 citation statements)

References 44 publications

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“…The circulating levels of fasting LPL were positively correlated with LPL at 120 min in both the 0%G and 0.8%G groups and negatively correlated with triglycerides at 120 min in the 0.8%G group, but not in the 0%G group. Consistent with these results, we showed that circulating fasting LPL level was a predictor of LPL activity and circulating LPL level during heparin treatment [17,[29][30][31][32]. Tsuzaki et al reported supportive ndings.…”

Section: Discussionsupporting

confidence: 90%

“…A previous report showed no change in HTGL activity after an infusion of lipid emulsions [35]. Plasma HTGL remains in a fairly static equilibrium with the vascular binding site HTGL levels, and that shifts in metabolic conditions do not rapidly change either HTGL activity or its interaction with these vascular sites [17]. In the present study, circulating levels of FFA decreased continuously; therefore, local FFA concentrations near the HTGL binding site might not have increased.…”

Section: Discussionsupporting

confidence: 43%

“…They found that fasting LPL mass levels may be useful as a predictor of increased postprandial triglycerides and that fasting LPL mass is a simple and easy-to-use marker in clinical practice [16]. On the other hand, no signi cant increase in LPL activity was found during chylomicron and VLDL overload after different kinds of food intake [17]. These discrepancies could be explained by differences in blood sampling time or test meals.…”

Section: Discussionmentioning

confidence: 99%

“…Fasting LPL mass levels may be useful as a predictor of postprandial triglyceride increases in fasting normotriglyceridemic subjects, and fasting LPL mass is a simple easy-to-use marker that can be applied in clinical practice [16]. On the other hand, no signi cant increase in LPL activity was found during chylomicron and very low-density lipoprotein (VLDL) overload after different kinds of food intake [17]. These ndings led us to ask what ingredients in food, such as KGM, increase circulating LPL.…”

Section: Introductionmentioning

confidence: 99%

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Attenuation of Lipid Metabolism by Novel Pleiotropic Effects of Konjac Glucomannan

Kimura

Yoshida

Tsunekawa

et al. 2020

Preprint

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Background: Recently, we showed that konjac glucomannan (KGM) inhibited rice gruel-induced postprandial elevation of plasma glucose and insulin. To extend that research, we investigated the effects of adding KGM to rice gruel on pre- and postprandial circulating lipoprotein lipase (LPL), glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1), and triglyceride concentrations. Methods: Twenty-four Japanese male subjects without diabetes or gastrointestinal diseases (aged 46–56 years) were included. Subjects received rice gruel containing 0% or 0.8% of KGM (0%G and 0.8%G, respectively). Blood samples were obtained at preload and 30, 60, and 120 min after receiving rice gruel with or without 0.8% KGM. LPL, GPIHBP1, and triglyceride concentrations in these samples were measured. Results: Circulating triglycerides were significantly reduced at 30 min and then returned to the fasting value in the subjects who received 0%G, whereas lower triglyceride levels were sustained in those who received 0.8%G. Although circulating levels of LPL and GPIHBP1 were significantly reduced for 0%G, they were increased for 0.8%G. We found a significant negative correlation between circulating levels of fasting LPL and triglycerides and a significant positive correlation between circulating levels of fasting LPL and GPIHBP1. A significant negative correlation between circulating levels of triglycerides at 120 min and fasting LPL was shown in the 0.8%G group, but not in the 0%G group. A significant positive correlation between circulating levels of LPL at 0 min and 120 min was shown in the 0%G and 0.8%G groups.Conclusions: The results demonstrated a novel pleiotropic effect of KGM. Supplementation of KGM powder in rice gruel sustained lower levels of triglycerides accompanying elevation of LPL and GPIHBP1, which attenuated lipid metabolism. Additionally, fasting LPL is a predictor of postprandial circulating levels of LPL.Trial registration: UMIN registration number: UMIN000025950; registered at February 1st. 2017; https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000029247

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 43%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Attenuation of Lipid Metabolism by Novel Pleiotropic Effects of Konjac Glucomannan

Kimura

Yoshida

Tsunekawa

et al. 2020

Preprint

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“…26, 27 This difference is exaggerated in post-heparin plasma, where LPL levels rise sharply but GPIHBP1 levels remain unchanged. We failed to detect GPIHBP1–LPL complexes in human serum or plasma, indicating that little of the circulating GPIHBP1 was associated with LPL.…”

Section: Discussionmentioning

confidence: 99%

An enzyme-linked immunosorbent assay for measuring GPIHBP1 levels in human plasma or serum

Miyashita

Fukamachi

Nagao

et al. 2018

Journal of Clinical Lipidology

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BACKGROUND GPIHBP1, a glycosylphosphatidylinositol (GPI)-anchored protein of capillary endothelial cells, transports lipoprotein lipase to the capillary lumen and is essential for the lipolytic processing of triglyceride-rich lipoproteins. OBJECTIVE Because some GPI-anchored proteins have been detected in plasma, we tested whether GPIHBP1 is present in human blood, and whether GPIHBP1 deficiency or a history of cardiovascular disease affected GPIHBP1 circulating levels. METHODS We developed two monoclonal antibodies against GPIHBP1and used the antibodies to established a sandwich ELISA to measure GPIHBP1 levels in human blood. RESULTS The GPIHBP1 ELISA was linear in the 8–500 pg/ml range and allowed the quantification of GPIHBP1 in serum and in pre- and post-heparin plasma (including lipemic samples). GPIHBP1 was undetectable in the plasma of subjects with null mutations in GPIHBP1. Serum GPIHBP1 median levels were 849 pg/ml (range: 740–1014) in healthy volunteers (n = 28) and 1087 pg/ml (range: 877–1371) in patients with a history of cardiovascular or metabolic disease (n = 415). There was an extremely small inverse correlation between GPIHBP1 and triglyceride levels (r = 0.109; P <0.0275). GPIHBP1 levels tended to be slightly higher in patients who had a major cardiovascular event after revascularization. CONCLUSION We developed an ELISA for quantifying GPIHBP1 in human blood. This assay will be useful to identify patients with GPIHBP1 deficiency and patients with GPIHBP1 autoantibodies. The potential of plasma GPIHBP1 as a biomarker for metabolic or cardiovascular disease is yet questionable but needs additional testing.

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A Review on Lipases: Sources, Assays, Immobilization Techniques on Nanomaterials and Applications

Fahim,

El-Khawaga,

Sallam

et al. 2024

BioNanoSci.

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Lipoprotein lipase does not increase significantly in the postprandial plasma

Abstract: No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.

Cited by 12 publications

References 44 publications

Attenuation of Lipid Metabolism by Novel Pleiotropic Effects of Konjac Glucomannan

Attenuation of Lipid Metabolism by Novel Pleiotropic Effects of Konjac Glucomannan

An enzyme-linked immunosorbent assay for measuring GPIHBP1 levels in human plasma or serum

A Review on Lipases: Sources, Assays, Immobilization Techniques on Nanomaterials and Applications

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