Lipoprotein apheresis downregulates IL-1α, IL-6 and TNF-α mRNA expression in severe dyslipidaemia

Stefanutti, Claudia; Mazza, Fabio; Pasqualetti, D; Giacomo, S. Di; Watts, Gerald F.; Massari, Maria Simona; Nève, Joel De; Morozzi, Claudia; Fischer, Moritz

doi:10.1016/j.atherosclerosissup.2017.05.028

Cited by 24 publications

(19 citation statements)

References 45 publications

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“…This may indicate that lipoprotein apheresis affects expression of IP‐10 differently in an in vivo and an ex vivo setting. Recently, Stefanutti et al demonstrated an impact on the messenger RNA of IL‐1α, IL‐6, and TNF in patients undergoing lipoprotein apheresis, indicating a possible regulatory effect on the expression of precursors in the chain of production of inflammatory mediators . This could contribute to explaining the observed differences between in effect on IP‐10 in in vivo and ex vivo studies.…”

Section: Discussionmentioning

confidence: 99%

Comparison of cytokine changes in three different lipoprotein apheresis systems in an ex vivo whole blood model

Hardersen

Enebakk

Christiansen

et al. 2019

J of Clinical Apheresis

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Introduction: Even if proprotein convertase subtilisin/kexin type 9 inhibitors have replaced lipoprotein apheresis in many patients, lipoprotein apheresis still is an important option in homozygous familial hypercholesterolemia, progressive atherosclerosis or when removal of lipoprotein(a) is indicated. Additional possible favorable effects beyond lipid lowering could include changes in the concentration of cytokines and improvement of hemorheology. Methods: We evaluated how whole blood adsorption, dextran sulfate plasma adsorption, and double filtration plasmapheresis lipoprotein apheresis systems affected cytokine concentrations, using a human whole blood ex vivo model differentiating the effect of the lipoprotein apheresis and plasma separation columns and describing temporal changes.

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Section: Discussionmentioning

confidence: 99%

Comparison of cytokine changes in three different lipoprotein apheresis systems in an ex vivo whole blood model

Hardersen

Enebakk

Christiansen

et al. 2019

J of Clinical Apheresis

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“…The production of ROS has been proved to be one of the important causes of atherosclerosis [35]. In addition, in ammatory molecules and apoptosis-related proteins, such as IL-6, TNF-α, BAX were abnormally elevated in patients with hyperlipidemia [35,36]. In animal study, simvastatin administration could attenuate brain oxidative stress during experimental sepsis [37].…”

Section: Production Of Ros In Ammation and Apoptosismentioning

confidence: 99%

Plasma Purification is Associated With Reduced Inflammation Factors and Down-regulation of Lipid Metabolic and ER Stress Proteins in Patients With Hyperlipidemia

Zhang

Deng

et al. 2020

Preprint

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Background Hyperlipidemia is a strong risk factor for arteriosclerosis and cardiovascular disease (CVD). Lipid-lowering therapy using drugs often results in many side effects. The aim of our study is to investigate the potential effects of non-drug therapy by double filtration plasmapheresis (DFPP) on lipid metabolic, endoplasmic reticulum (ER) stress and apoptosis related proteins in peripheral blood mononuclear cells (PBMC) before and after lipid clear out in patients with hyperlipidemia. MethodsLipid metabolic [CD36, propotein convertase subtilisin/kexin type 9 (PCSK9) and LDL receptor], ER stress (GRP78, CHOP, ATF4, EIF2a) and apoptosis related (Bcl-2, BAX, and Caspase-3) proteins were assayed by western blot, reactive oxygen species (ROS) measured by flow cytometry (FCM) and serum inflammatory factors were detected by ELISA. Results35 patients with hyperlipidemia were selected into this study. Compared with pre-DFPP, most of lipid metabolic parameters as cholesterol, triglyceride, LDL, lipoprotein a [Lp(a)] and small dense LDL cholesterol (sdLDL) reduced. DFPP process was associated with the down-regulation of lipid metabolic, ER stress and apoptosis proteins, also resulted in the decrease in ROS and serum inflammatory factors release. Conclusion DFPP owns the capacity of lipid-lowering, also can regulate lipid metabolic, ER stress and apoptosis related proteins in PBMC and reduce inflammatory factors in patients with hyperlipidemia (ClinicalTrials.gov number, NCT03491956). Footnote: Xiao Meng Zhang and Hao Deng contribute equally to this paper.

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“…The production of ROS has been proven to be an important cause of atherosclerosis [35]. In addition, in ammatory molecules and apoptosis-related proteins, such as IL-6, TNF-α, and BAX, were abnormally elevated in patients with hyperlipidaemia [35,36].…”

Section: Ros Production In Ammation and Apoptosismentioning

confidence: 99%

Plasma purification is associated with reduced inflammation factors and down-regulation of lipid metabolic and ER stress proteins in patients with hyperlipidemia

Zhang

Deng

et al. 2020

Preprint

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Background Hyperlipidaemia is a strong risk factor for arteriosclerosis and cardiovascular disease (CVD). Therapy with lipid-lowering drugs often results in many side effects. The aim of our study was to investigate the potential effects of non-drug therapy with double-filtration plasmapheresis (DFPP) on lipid metabolism-, endoplasmic reticulum (ER) stress- and apoptosis-related proteins in peripheral blood mononuclear cells (PBMCs) before and after lipid clearance in patients with hyperlipidaemia.Methods Thirty-five patients with hyperlipidaemia were selected for this study. Proteins related to lipid metabolism [CD36, proprotein convertase subtilisin/kexin type 9 (PCSK9) and LDL receptor], ER stress (GRP78, CHOP, ATF4, EIF2a) and apoptosis (Bcl-2, BAX, and Caspase-3) were assayed by western blot, reactive oxygen species (ROS) were measured by flow cytometry (FCM), and serum inflammatory factors were detected by ELISA.Results Compared with their pre-DFPP values, the values of most lipid metabolic parameters, such as cholesterol, triglycerides, LDL, lipoprotein a [Lp(a)] and small dense LDL cholesterol (sdLDL), were reduced after DFPP. DFPP was associated with the downregulation of proteins related to lipid metabolism, ER stress and apoptosis, which also resulted in decreased ROS and serum inflammatory factor release.Conclusion DFPP has lipid-lowering activity and can also regulate lipid metabolism-, ER stress- and apoptosis-related proteins in PBMCs and reduce the levels of inflammatory factors in patients with hyperlipidaemia (ClinicalTrials.gov number: NCT03491956).

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Lipoprotein apheresis downregulates IL-1α, IL-6 and TNF-α mRNA expression in severe dyslipidaemia

Cited by 24 publications

References 45 publications

Comparison of cytokine changes in three different lipoprotein apheresis systems in an ex vivo whole blood model

Comparison of cytokine changes in three different lipoprotein apheresis systems in an ex vivo whole blood model

Plasma Purification is Associated With Reduced Inflammation Factors and Down-regulation of Lipid Metabolic and ER Stress Proteins in Patients With Hyperlipidemia

Plasma purification is associated with reduced inflammation factors and down-regulation of lipid metabolic and ER stress proteins in patients with hyperlipidemia

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