Lipoprotein and apoprotein profile of Japanese quail

Oku, Hirosuke; Ishikawa, Makoto; Nagata, Junichi; Toda, Takayoshi; Chinen, Isao

doi:10.1016/0005-2760(93)90212-r

Cited by 14 publications

(4 citation statements)

References 20 publications

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“…This technique is extremely time‐consuming, requiring 6‐30 hours of very high‐speed centrifugation with specialized equipment 16 . It is not offered commercially for animals, but has been used for research in pigeons, chickens, quails, geese, Quaker parrots, and cockatiels 6‐9,17‐20 . While this method allows separation and isolation of lipoprotein classes and subclasses, it does not allow the determination of lipoprotein particle numbers and sizes.…”

Section: Introductionmentioning

confidence: 99%

Lipoprotein characterization in Quaker parrots (Myiopsitta monachus) using gel‐permeation high‐performance liquid chromatography

Beaufrère

Gardhouse

Ammersbach³

2020

Veterinary Clinical Pathol

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Background: Lipid accumulation disorders, such as atherosclerosis and hepatic lipidosis, are common in psittacine birds and associated with various dyslipidemias. Gel-permeation high-performance liquid chromatography (GP-HPLC) is a reference method for advanced lipoprotein profiling based on particle size separation, followed by an analysis of lipid contents. Objectives: The objectives were to (a) characterize Quaker parrot lipoproteins using a commercial GP-HPLC method (Liposearch panel), and (b) obtain preliminary information on the reliability of the Friedewald formula for low-density lipoprotein-cholesterol (LDL-C) measurements. Methods: Plasma samples were collected from 12 fasted healthy Quaker parrots. Cholesterol concentrations, triglyceride concentrations, particle sizes, and particle numbers were determined by GP-HPLC for four classes and 20 sub-fractions of lipoproteins. The LDL-C concentrations obtained using the Friedewald formula and direct measurements were compared with Bland-Altman plots. Alternate formulas were determined using multiple linear regression. Results: High-density lipoprotein (HDL) was the predominant lipoprotein in Quaker parrots, and most particles were of medium-to-small sizes belonging to two subfractions (average size, 10.6 nm). LDL was the second most common lipoprotein and included large-to-small particles belonging to three sub-fractions (average size, 24.9 nm). Very-low-density lipoproteins (VLDL) and portomicrons were present in low concentrations. The Friedewald formula underestimated LDL-C concentrations with a significant bias of 0.44 mmol/L. An alternate formula was proposed: LDL-C = 0.75*Non-HDL-C. Conclusions: GP-HPLC allowed unprecedented characterization of plasma lipoproteins in Quaker parrots. Characterizing psittacine lipoprotein is useful for validation and interpretation of routine clinical tests as well as for use in epidemiologic and experimental research on psittacine lipid accumulation disorders. K E Y W O R D S cholesterol, Friedewald formula, HDL, LDL, lipoprotein particle number, triglycerides How to cite this article: Beaufrère H, Gardhouse S, Ammersbach M. Lipoprotein characterization in Quaker parrots (Myiopsitta monachus) using gel-permeation highperformance liquid chromatography.

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Section: Introductionmentioning

confidence: 99%

Lipoprotein characterization in Quaker parrots (Myiopsitta monachus) using gel‐permeation high‐performance liquid chromatography

Beaufrère

Gardhouse

Ammersbach³

2020

Veterinary Clinical Pathol

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“…Further, the nature of the lesions is similar to that in humans (Shih et al, 1983). Altogether, the reproducibility of advanced lesions, along with a well-defined genetic background, makes the atherosclerosis-susceptible Japanese quail attractive in dyslipidemia and atherosclerosis research (Ojerio et al, 1972;Shih et al, 1983;Oku et al, 1993).…”

Section: Quailsmentioning

confidence: 93%

“…2) (Hammad et al, 1998), diet-induced hypercholesterolemia is associated with a significant shift in LDL and VLDL in serum (Ojerio et al, 1972). Increased levels of plasma cholesterol correlate with an increased frequency and severity of atherosclerotic lesions (Oku et al, 1993).…”

Section: Quailsmentioning

confidence: 99%

Animal models for the atherosclerosis research: a review

et al. 2011

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“…The lipoprotein composition has been examined in turkey [2], chicken [2], and Japanese quail [10], but little is known about lipoproteins in duck embryos and newly hatched ducklings. We [11] recently reported, however, that duck embryos from young parents have significantly less liver lipid accumulation as compared with duck embryos from older parents; therefore, suggesting that circulating lipoproteins may be altered.…”

Section: Introductionmentioning

confidence: 99%

The Circulating Levels of Lipoproteins in Embryos and Newly Hatched Ducklings Change with Parental Age<sup>1</sup>

2002

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The purpose of this study was to investigate the effects of duck breeder age on circulating very low density lipoprotein (VLDL) and low-density lipoprotein (LDL) levels in embryonic (day 25 of incubation) and newly hatched ducklings (hatch and 1, 2, 3, and 5 days after hatch). In this study, embryos and ducklings from one breeder flock were monitored at breeder ages 24 weeks (young breeders), 31 weeks (mature breeders and near peak egg production), and 47 weeks (after peak egg production). Embryos and newly hatched ducklings from young breeders (24 weeks of age) displayed lower levels of VLDL and LDL. The composition of VLDL was affected by breeder age such that embryos and ducklings from young breeders had less cholesterol ester/protein and triglyceride/protein ratios. Even though the overall levels were suppressed in embryos and newly hatched ducklings from 24-week-old breeders, the overall percentage of triglyceride within VLDL and LDL particles was higher. These data would suggest that breeder age affects concentration and composition of both VLDL and LDL in embryos and newly hatched ducklings.

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Lipoprotein and apoprotein profile of Japanese quail

Cited by 14 publications

References 20 publications

Lipoprotein characterization in Quaker parrots (Myiopsitta monachus) using gel‐permeation high‐performance liquid chromatography

Lipoprotein characterization in Quaker parrots (Myiopsitta monachus) using gel‐permeation high‐performance liquid chromatography

Animal models for the atherosclerosis research: a review

The Circulating Levels of Lipoproteins in Embryos and Newly Hatched Ducklings Change with Parental Age<sup>1</sup>

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