Lipopolythiourea/DNA interaction: A biophysical study

Kral, Teresa; Leblond, Jeanne; Hof, Martin; Scherman, Daniel; Herscovici, Jean; Mignet, Nathalie

doi:10.1016/j.bpc.2010.02.011

Cited by 16 publications

(14 citation statements)

References 36 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this technique, addition of increasing amounts of lipothiourea slows down DNA mobility as soon as an interaction occurs. Using FCS, we evidenced an interaction at a ratio of one thiourea per phosphate and a full condensation at a ratio of 5 nmol lipid/μg DNA for the A C10 S Dig L Ser P diol compound (number 3 in Table 1, also called DDSTU in the literature) [35]. Increasing the lipid length from C 10 to C 14 (compounds 3 , 2 and 1 ), reduced the efficiency of the interaction with DNA for the serinol series.…”

Section: Resultsmentioning

confidence: 99%

“…The two lead candidates, compound 3 , (A C10 S Dig L Ser P diol ), and compound 6 (A C14 L Lys P diolP ) which were found to be the best transfecting agents from their series, are also the lipids which showed the strongest interaction with DNA [35]. In addition, these lipids were also the ones which interacted at a lower lipid/DNA ratio (see Table 1, red boxes).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Lipothioureas as Lipids for Gene Transfection: A Review

et al. 2011

Self Cite

View full text Add to dashboard Cite

Non-viral gene therapy requires innovative strategies to achieve higher transfection efficacy. A few years ago, our group proposed bioinspired lipids whose interaction with DNA was not based on ionic interactions, but on hydrogen bonds. We thus developed lipids bearing a thiourea head which allowed an interaction with DNA phosphates through hydrogen bonds. After a proof of concept with a lipid bearing three thiourea functions, a molecular and cellular screening was performed by varying all parts of the lipids: the hydrophobic anchor, the spacer, the linker, and the thiourea head. Two lipothiourea-based structures were identified as highly efficient in vitro transfecting agents. The lipothioureas were shown to reduce non specific interactions with cell membranes and deliver their DNA content intracellularly more efficiently, as compared to cationic lipoplexes. These lipids could deliver siRNA efficiently and allowed specific cell targeting in vitro. In vivo, thiourea lipoplexes presented a longer retention time in the blood and less accumulation in the lungs after an intravenous injection in mice. They also induced luciferase gene expression in muscle and tumor after local administration in mice. Therefore, these novel lipoplexes represent an excellent alternative to cationic lipoplexes as transfecting agents. In this review we will focus on the structure activity studies that permitted the identification of the two most efficient thiourea lipids.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Lipothioureas as Lipids for Gene Transfection: A Review

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…The fluorescence lifetime gives information on the local environment both PicoGreen and Hoechst label. As shown earlier [23,28], it can be interpreted as a mark of DNA tight compaction.…”

Section: Fluorescence Spectroscopymentioning

confidence: 72%

“…To explore the dibutyltin citrate complex-DNA interactions, a single-molecule detection technique that combines simultaneous time-correlated single photon counting time tag mode with fluorescence correlation spectroscopy (TCSPC-FCS) was employed, as reported earlier [21][22][23] Mainly, there are two readout parameters the residence time (s res ) and apparent particle number (PN) contain information about the diffusion coefficient and the concentration of the fluorescently labeled molecules, respectively [24]. PN is described by the equation PN = C 9 V 9 N A , where C is molarity of detected molecules (DNA), V is confocal volume (1 fL), and N A is the Avogadro constant.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Interaction of new butyltin citrate complex with lipid model membrane and DNA

Pruchnik

Kral

Hof

2014

J Therm Anal Calorim

Self Cite

View full text Add to dashboard Cite

Organotin(IV) complexes show a wide variety of biological activity: bactericidal, fungicidal, caricidal, and pesticidal. Many of them are more effective than traditional heavy metal anticancer drugs. The aim of the present study was to investigate the interaction of a new dibutyltin complex with citric acid (DBTC) with lipid membrane and plasmid DNA. The effect of this compound on the multilamellar liposomes formed with dipalmitoylphosphatidylcholine, dimyristoylphosphatidylcholine, and dimyristoylphosphatidylethanolamine was studied mainly by the means of differential scanning calorimetry and additionally by the steady-state fluorimetry. Calorimetrical results together with fluorescence spectroscopy suggest that investigated complex interacts with lipids and probably locates itself in the hydrophilic part of the membrane. The effect of DBCT on plasmid DNA was investigated using single-molecule florescence technique and time-correlated single photon counting fluorescence correlation spectroscopy. We could conclude then that DBTC-DNA interaction occurs due to the interaction with the DNA phosphate group and charge neutralization which then leads to DNA compaction.

show abstract

“…From the classical UV-vis spectrophotometry or competition dialysis assay to the renewed gel mobility electrophoresis assay or the powerful tandem HPLC-MS or capillary electrophoresis-mass spectrometry, the number of new specific assays and methodologies is overwhelming (Tian et al, 2005). We will describe below some of the most useful techniques, with the exception of separation methods such as chromatography and conventional or capillary electrophoresis, which would require a whole chapter; for further information on this subject, see (Baba, 1999;Krylova et al, 2010;Kral et al, 2010;Koster et al, 2008;Araya et al, 2007;Su et al, 2007a;Su et al, 2007b).…”

Section: Analytical Techniques To Evidence Drug-dna Interactionsmentioning

confidence: 99%