Lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification

Abstract

Twelve motile Aeromonas strains have been examined with respect to the hexose and heptose monosaccharide residures present in the core region of their cell wall lipopolysaccharides. These strains were divided into three distinctly separate groups on the basis of the various combinations of hexose and heptose residues. The assignment of a strain to any one of the three groups furnishes a distribution which is substantially the same as that recently reported in a computerbased numerical analysis. All strains tested which were previoulsy named A. liquefaciens fall into the same group.

Structure of the lipopolysaccharide core isolated from a human strain of Aeromonas hydrophila

“…Mild acetic acid hydrolysis followed by Sephadex chromatography of the supernatant solution gave two carbohydrate-containing components corresponding to an SR-polysaccharide with a KaV value between 0.45 and 0.55 and a core oligosaccharide with K,, value between 0.6 and 0.7 (for definition of the Kay value see [8]). The amounts of these components averaged 15% and 85% of the recovered carbohydrate respectively.…”

“…Cells used to isolate the lipopolysaccharide were grown at 25°C to late stationary phase (24 h) in Trypticase Soy Broth without dextrose (25 1) as previously described [8]. High-voltage paper electrophoresis was conducted for 90 min in a Shandon flat-bed electrophoresis apparatus using a buffer of 5:2:43 (v/v/v)pyridine:aceticacid:water at pH 5.4, 2 kV and 170 mA.…”

“…Cells used to isolate the lipopolysaccharide were grown at 25°C to late stationary phase (24 h) in Trypticase Soy Broth without dextrose (25 1) as previously described [8].…”

“…Recent investigations by traditional biochemical reactions [7] and by analysis of the composition of the core oligosaccharide of the cell-surface lipopolysaccharide [8] have indicated three major sub-groupings. The detailed core structures of A .…”

Quantitative and Qualitative Studies of Aeromonas salmonicida Bacteriophage

“…Cell wall LPS of rough and smooth strains was extracted and purified using the hot phenol technique outlined by Shaw & Hodder (1978). A modified technique, using 0.5% (w/v) MgCI, to precipitate LPS, was necessary for G-phase cells.…”