Lipopolysaccharide Primes Human Basophils for Enhanced Mediator Release: Requirement for Plasma Co-factor and CD14

Iida, M.; Hirai, Kazumitsu; Shinohara, Shunji; Yamaguchi, M.; Takaishi, Toshiaki; Sakamoto, Yoshio; Ito, Kazuko; Morita, Yukiko

doi:10.1006/bbrc.1994.2323

Cited by 14 publications

(9 citation statements)

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“…Only a few studies have examined the responses of basophils to LPS, most of which have shown that in mixed cell suspensions LPS primes or enhances basophil histamine release (20,28). However, one study showed that purified basophils did not make IL-8 mRNA when stimulated with LPS, but did make IL-8 mRNA in response to control stimuli (43).…”

Section: Discussionmentioning

confidence: 99%

Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses

Sabroe

Jones

Usher

et al. 2002

The Journal of Immunology

347

324

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Leukocyte responsiveness to LPS is dependent upon CD14 and receptors of the Toll-like receptor (TLR) family. Neutrophils respond to LPS, but conflicting data exist regarding LPS responses of eosinophils and basophils, and expression of TLRs at the protein level in these granulocyte lineages has not been fully described. We examined the expression of TLR2, TLR4, and CD14 and found that monocytes expressed relatively high levels of cell surface TLR2, TLR4, and CD14, while neutrophils also expressed all three molecules, but at low levels. In contrast, basophils expressed TLR2 and TLR4 but not CD14, while eosinophils expressed none of these proteins. Tested in a range of functional assays including L-selectin shedding, CD11b up-regulation, IL-8 mRNA generation, and cell survival, neutrophils responded to LPS, but eosinophils and basophils did not. In contrast to previous data, we found, using monocyte depletion by negative magnetic selection, that neutrophil responses to LPS were heavily dependent upon the presence of a very low level of monocytes, and neutrophil survival induced by LPS at 22 h was monocyte dependent. We conclude that LPS has little role in the regulation of peripheral blood eosinophil and basophil function, and that, even in neutrophils, monocytes orchestrate many previously observed leukocyte LPS response patterns.

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Section: Discussionmentioning

confidence: 99%

Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses

Sabroe

Jones

Usher

et al. 2002

The Journal of Immunology

347

324

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“…While both peripheral blood basophils 59 and RBL cells 60 appear to express TLR-4, RBL cells are not activated by LPS, possibly because they lack the essential co-receptor CD14. 60 However, LPS also does not directly activate peripheral blood basophils, 61 despite the apparent presence of CD14, 62 whereas it appears to synergize with IgE-dependent stimuli. 61 We have not found any information about the presence of TLR-2 on RBL cells.…”

Section: Rbl Cells: Mcs or Basophils?mentioning

confidence: 99%

RBL cells as models for in vitro studies of mast cells and basophils

Falcone

Wan

Barwary

et al. 2018

Immunological Reviews

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Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically manipulated, have provided advantages over primary MCs, in particular for molecular studies relying on genetic screening. Furthermore, the ability to generate clones that stably express proteins of interest, for example, a human receptor, have marked the RBL cells as an attractive MC model for drug screening. Indeed, 3 RBL reporter cell lines (RS-ATL8, NFAT-DsRed, and NPY-mRFP) have been generated providing useful models for drug and allergen screening. Similarly, RBL cells stably expressing the human MrgprX2 receptor provide a unique paradigm for analyzing ligand interactions and signaling pathways of the unique human receptor. Finally, transient co-transfections of RBL cells allow functional genomic analyses of MC secretion by combining library screening with simultaneous expression of a reporter for exocytosis. RBL cells thus comprise powerful tools for the study of intracellular membrane trafficking and exocytosis and the detection of allergens, vaccine safety studies and diagnosis of allergic sensitization. Their recent uses as an investigative tool are reviewed here.

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“…CD14 is a glycosylphosphatidylinositol (GPI)-linked protein present on monocytes and PMN that binds LPS (1) and plays a crucial role in mediating cellular responses to LPS (8). Blockade of CD14 with monoclonal antibodies strongly reduces many cellular responses to LPS (8,9) and completely eliminates the adhesive response of PMN to LPS (1)(2)(3).…”

mentioning

confidence: 99%

Plasma Lipopolysaccharide-binding Protein Is Found Associated with a Particle Containing Apolipoprotein A-I, Phospholipid, and Factor H-related Proteins

Park¹,

Wright²

1996

Journal of Biological Chemistry

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Neutrophils exhibit a dramatic enhancement of integrin-mediated cell adhesion in response to lipopolysaccharide (LPS). This response requires CD14 on the neutrophil and plasma proteins in solution. We have purified the factor from plasma that facilitates the adhesive response of neutrophil to LPS by using a combination of affinity and ion-exchange chromatography. Previous work has shown that the activity is associated with apolipoprotein A-I (apoA-I), and here we show that this activity is associated with an apoA-I-bearing complex of protein and phospholipid. Native polyacrylamide gel electrophoresis (PAGE) analysis showed a ladder of bands in the M r 200,000 region, and electron microscopy revealed round, indented particles of 11.4 ؎ 0.12 nm in diameter. Characterization of these particles revealed a density of 1.219 -1.264 g/ml and ϳ10 molecules of lipid phosphate per M r 200,000 complex. SDS-PAGE showed that each of the bands seen in native PAGE was composed of several polypeptides. These were identified as apoA-I, LPS binding protein (LBP), and factor Hrelated proteins (FHRPs). Physical association of apoA-I, LBP, and FHRP in these particles was further confirmed using double immunodiffusion, and association of LBP and FHRP in plasma was confirmed by coimmunoprecipitation. FHRPs are the numerically dominant protein components in these particles, and all plasma FHRP-1 appears to be associated with these particles. We suggest that FHRPs may be the defining constituent of this novel "lipoprotein" particle.

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Lipopolysaccharide Primes Human Basophils for Enhanced Mediator Release: Requirement for Plasma Co-factor and CD14

Cited by 14 publications

References 0 publications

Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses

Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses

RBL cells as models for in vitro studies of mast cells and basophils

Plasma Lipopolysaccharide-binding Protein Is Found Associated with a Particle Containing Apolipoprotein A-I, Phospholipid, and Factor H-related Proteins

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