Lipopolysaccharide O-antigen molecular and supramolecular modifications of plant root microbiota are pivotal for host recognition

Vanacore, Adele; Vitiello, Giuseppe; Wanke, Alan; Cavasso, Domenico; Clifton, Luke A.; Mahdi, Lisa K.; Campanero-Rhodes, María Asunción; Solı́s, Dolores; Wuhrer, Manfred; Nicolardi, Simone; Molinaro, Antonio; Marchetti, Roberta; Zuccaro, Alga; Paduano, Luigi; Silipo, Alba

doi:10.1016/j.carbpol.2021.118839

Cited by 15 publications

(10 citation statements)

References 48 publications

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Section: Discussionmentioning

confidence: 99%

“…The O-methylation of LOS O-antigen residues, Rhamnose included, is instead more frequently found in plant-associated bacteria, either Exopolysaccharides or Lipopolysaccahrides. 14,38,39 As for the core OS, this was characterized by an extensive, multiple non-stochiometric O-methylation of the sugar residues, distributed along the whole saccharide chain. This included the novel and unprecedented O-methylation at position O5 on the alternative terminal Kdo/Ko units and the extreme non-reducing core region that was capped by a rhamno-trisaccharide chain extensively O-methylated, which further decreases the hydrophilicity of the outermost portion of the LOS (Figure 4).…”

Section: ■ Discussionmentioning

confidence: 99%

“…Ko has been so far found in a few bacterial species, like Burkholderia and Acinetobacter , never O -methylated. The O -methylation of LOS O -antigen residues, Rhamnose included, is instead more frequently found in plant-associated bacteria, either Exopolysaccharides or Lipopolysaccahrides. ,, …”

Section: Discussionmentioning

confidence: 99%

“…In addition, the hydrophilic sugar backbone was amply scaled down by methyl groups. It is worth noting that this vast and spread methylation could be deemed an option to modulate the glycans chemical structure and biophysical properties, facilitate the molecular dialogue with the host, mediate symbiotic and environmental interactions, and constitute a conserved target of the host innate immune system. , In this frame, we have already demonstrated the ability of M. extorquens PA1 to produce exopolysaccharide mixtures altered if grown in the presence of methanol and with a variable degree of methylation that influenced the 3D structure, supramolecular assets, diffusion properties, and hydrodynamic radius, thus contributing to increased methanol tolerance and cell stability.…”

Section: Discussionmentioning

confidence: 99%

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Expanding Knowledge of Methylotrophic Capacity: Structure and Properties of the Rough-Type Lipopolysaccharide from Methylobacterium extorquens and Its Role on Membrane Resistance to Methanol

Lorenzo

Nicolardi

Marchetti

et al. 2023

JACS Au

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The ability of Methylobacterium extorquens to grow on methanol as the sole carbon and energy source has been the object of intense research activity. Unquestionably, the bacterial cell envelope serves as a defensive barrier against such an environmental stressor, with a decisive role played by the membrane lipidome, which is crucial for stress resistance. However, the chemistry and the function of the main constituent of the M. extorquens outer membrane, the lipopolysaccharide (LPS), is still undefined. Here, we show that M. extorquens produces a rough-type LPS with an uncommon, non-phosphorylated, and extensively O-methylated core oligosaccharide, densely substituted with negatively charged residues in the inner region, including novel monosaccharide derivatives such as O-methylated Kdo/Ko units. Lipid A is composed of a non-phosphorylated trisaccharide backbone with a distinctive, low acylation pattern; indeed, the sugar skeleton was decorated with three acyl moieties and a secondary very long chain fatty acid, in turn substituted by a 3-O-acetyl-butyrate residue. Spectroscopic, conformational, and biophysical analyses on M. extorquens LPS highlighted how structural and tridimensional features impact the molecular organization of the outer membrane. Furthermore, these chemical features also impacted and improved membrane resistance in the presence of methanol, thus regulating membrane ordering and dynamics.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expanding Knowledge of Methylotrophic Capacity: Structure and Properties of the Rough-Type Lipopolysaccharide from Methylobacterium extorquens and Its Role on Membrane Resistance to Methanol

Lorenzo

Nicolardi

Marchetti

et al. 2023

JACS Au

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“…LPS possess long O-antigen that restrict the initial plant recognition, thereby enabling elicitation of innate immunity and helping to evade successfully into the host (Ranf et al, 2015; Rapicavoli et al, 2018). Whereas the recent plant immunology study also demonstrated that acetylated LPS in Arabidopsis thaliana protects the LPS from immune recognition (Vanacore et al, 2022), and it is found to activate biphasic production of reactive oxygen species (ROS) (Shang-Guan et al, 2018). In addition, LPS has been discovered to be a fundamental factor that increases bacterial viability and aids in inter-organismic interaction as well.…”

Section: Introductionmentioning

confidence: 99%

Systematic hyper-variation and evolution at a lipopolysaccharide locus in the population of Xanthomonas species that infect rice and sugarcane

Singh

Bansal

Patil

2022

Preprint

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Advent of high throughput sequencing and population genomics is enabling researchers to investigate selection pressure at hyper-variable genomic loci encoding pathogen-associated molecular patter (PAMP) molecules like lipopolysaccharide (LPS) in an unprecedented manner. Xanthomonas is a model group of phytopathogenic bacteria that infects host in tissue-specific manner. Our in-depth investigation revealed that the successful emergence of lineages infecting major cereals and grasses like rice, sugarcane, and wheat was mediated by acquisition and later replacement of an ancestral type (BXO8) of LPS cassette by distinct one. In the population of the rice xylem pathogen, X. oryzae pv. oryzae (Xoo), the BXO8 is replaced by a distinct BXO1 type of cassette. Alternatively, in diverse Xanthomonas species that infect sugarcane, the BXO8 ancestral cassette has been replaced by yet another kind of Xvv type of LPS cassette, suggesting convergent evolution at an LPS locus mediated by horizontal gene transfer (HGT) events. Aside from xylem, two closely related lineages of X. oryzae that infect parenchyma tissue of rice and Leersia hexandra grass have acquired an LPS cassette from Xanthomonas pathogens that infect citrus, walnut, and strawberry parenchyma, indicating yet another instance of parallel evolution facilitated by HGT. Our targeted and mega-population-based genome dynamic studies revealed potential role of acquisition of specific types of LPS cassettes in the emergence and evolution of tissue specificity in Xanthomonas. Additional cellular, molecular, genetic, and plant studies will help us figure out how a distinct type of LPS help Xanthomonas pathovars and lineages adapt to parenchyma and xylem tissues.

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Synthesis of Unprecedented α/β‐Alternate (1→4)‐Glucans via Stereoselective Iterative Glycosylation

Yang

Sun

et al. 2023

Chemistry A European J

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Glucans are major biomaterials on the earth, with α‐(1→4)‐glucans (i. e., amylose) and β‐(1→4)‐glucans (i. e., cellulose) being the most abundant ones, which are relevant to energy storage and structural function, respectively. Interestingly, (1→4)‐glucans with alternate α/β‐linkages, namely herewith amycellulose, have never been disclosed in nature. Here we report a robust glycosylation protocol for the stereoselective construction of the 1,2‐cis‐α‐ and 1,2‐trans‐β‐glucosidic linkages, which employs an optimal combination of glycosyl N‐phenyltrifluoroacetimidates as donors, TMSNTf2 as promoter, CH2Cl2/nitrile or CH2Cl2/THF as solvents. A broad substrate scope has been demonstrated by coupling five imidate donors with eight glycosyl acceptors, in which most of the glycosylations lead to high yield and exclusively 1,2‐cis‐α‐ or 1,2‐trans‐β‐selectivity. Applying this glycosylation protocol and with an iterative manner, the unprecedented α/β‐alternate (1→4)‐glucans up to a 16‐mer have been synthesized. Differently from amylose, that adopts a compact helicoidal arrangement, the synthetic amycellulose features an extended ribbon‐like conformation, comparable to the extended shape of cellulose.

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Lipopolysaccharide O-antigen molecular and supramolecular modifications of plant root microbiota are pivotal for host recognition

Cited by 15 publications

References 48 publications

Expanding Knowledge of Methylotrophic Capacity: Structure and Properties of the Rough-Type Lipopolysaccharide from Methylobacterium extorquens and Its Role on Membrane Resistance to Methanol

Expanding Knowledge of Methylotrophic Capacity: Structure and Properties of the Rough-Type Lipopolysaccharide from Methylobacterium extorquens and Its Role on Membrane Resistance to Methanol

Systematic hyper-variation and evolution at a lipopolysaccharide locus in the population of Xanthomonas species that infect rice and sugarcane

Synthesis of Unprecedented α/β‐Alternate (1→4)‐Glucans via Stereoselective Iterative Glycosylation

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