Lipopolysaccharide in the pathogenicity of Treponema hyodysenteriae

Nuessen, M E

doi:10.31274/rtd-180813-4771

Cited by 9 publications

(13 citation statements)

References 61 publications

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“…Alternatively, invasion is preceded by initial damage to tight junctions (Kang and Olander, 1990;ter Huurne and Gaastra, 1995). In addition, the host's immune response might exacerbate the lesions (Hontecillas et al, 2005;Nuessen et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Consecutive pathological and immunological alterations during experimentally induced swine dysentery – A study performed by repeated endoscopy and biopsy samplings through an intestinal cannula

Jacobson

Lindberg

Jonasson

et al. 2007

Research in Veterinary Science

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The development of intestinal lesions after inoculation with Brachyspira hyodysenteriae was followed by repeated endoscopy and biopsy sampling through a caecal cannula. Seven eight-week-old pigs were cannulated and inoculated, two were cannulated but not inoculated, and two pigs were inoculated but not cannulated. Endoscopy, biopsy, and blood sampling to determine SAA (serum amyloid A), haptoglobin, cortisol, and WBC counts were performed at scheduled time-points. At the third day of disease, endoscopy showed a hyperaemic, perturbed mucosa and excessive amount of mucus. Histologically, crypt hyperplasia, depletion of goblet cell mucus, and erosions were noted. Simultaneously, elevated acute phase proteins and circulating monocytes, and decreased number of intraepithelial CD3(+) cells were observed. After five days the pigs recovered. Intestinal lesions were demarcated and interspersed among apparently normal mucosa and blood parameters returned to initial values. Endoscopy through an intestinal cannula made it possible to follow the development of intestinal alterations in vivo and describe the sequential events during the course of swine dysentery. The number of animals used in a study could thus be minimised and the precision of the experiment increased.

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Section: Introductionmentioning

confidence: 99%

Consecutive pathological and immunological alterations during experimentally induced swine dysentery – A study performed by repeated endoscopy and biopsy samplings through an intestinal cannula

Jacobson

Lindberg

Jonasson

et al. 2007

Research in Veterinary Science

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“…suggested the possible involvement of endotoxinlike activity associated with those organisms (3,5,10,11,19). However, no precise chemical characterization was performed in these studies.…”

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confidence: 95%

Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi

Takayama¹,

Rothenberg²,

Barbour³

1987

Infect Immun

220

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We were unable to deMonstrate the presence of the classic enterobacterium-type lipopolysaccharide in the cells of the Lyme disease spirochete, Borrelia burgdorferi B31. This finding was primarily based on chemical analysis and the absence of free lipid A upon mild acid hydrolysis of the appropriate cell extracts. These re4ults do not preclude the possible existence of an unusual lipopolysaccharide-like compound(s) in B. burgdorferi. Beck et al. (4) recently reported the presence of lipopolysaccharide (LPS) irq the Lyme disease spirochete (Borrelia burgdorferi). We wanted to confirm their results and then examine the nature of the lipid A moiety of LPS from this source. We extracted the cells of B. burgdorferi B31 by two well-known methods used in the isolation of LPS from gram-negative bacteria. The appropriate fractions obtained by these two methods were hydrolyzed under mild acid conditions and examined by thin-layer chromatography (TLC) for the presence of free lipid A. Chemical analyses for the presence of 2-keto-3-deoxyoctonate, glucosamine, and hydroxy fatty acids were performed on the extracts. We found no evidence for the presence of lipid A in any of the fractions tested. Thus, we must conclude that the classic LPS associated with gram-negative bacteria is absent in cells of B. burgdorferi.Cells of B. burgdorferi B31 (ATCC 35210) were grown in BSK II medium (1), washed three times with 50 mM Tris (pH 7.4)-150 mM NaCI-5 mM MgCI2 (2), and lyophilized. In one experiment, the extraction method of Galanos et al. (7) with modifications was used (12,14). The results of this extraction are summarized in Table 1. Almost all of the extractable material was found in the 90% aqueous ethanol fraction, whereas very little material appeared in the phenol-waterchloroform-petroleum ether fraction, which should contain the rough-type LPS (R-LPS). This latter fraction was hydrolyzed in 0.1 N HCI as previously described for the preparation of monophosphoryl lipid A (14), and 30 ,ug of the organic extract was analyzed by TLC for the presence of lipid A (12,14). A mixture of hexa-, penta-, and tetraacyl monopliosphoryl lipid A (from LPS of Salmonella typhimurium) was used as a chromatographic standard (13). There was no evidence of the presence of free lipid A in this sample, indicating the absence of R-LPS in the original extract (phenol-water-chloroform-petroleum ether).Previous studies of lipids X and Y (monosaccharide precursors of LPS) showed that much of X and Y was extracted into the aqueous ethanol fraction (15,16). Thus, it is conceivable that the 113.2 mg of the 90% ethanol extract (Table 1) might contain the R-LPS. This material was initially fractionated by solvent precipitation to yield 79.9 mg of an acetone-insoluble glycolipid-phospholipid fraction. This * Corresponding author. fraction was further purified by preparative TLC on Silica Gel G (Merck & Co., Inc.) by a solvent system of chloroform-methanol-water (65:25:4). Five major fractions (designated A to E) were obtained. Each of these fractions was acid hy...

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“…Several factors which may be associated with the virulence of T. hyodysenteriae have been identified. These include lipopolysaccharide (LPS), [4][5][6][7] a haemolysin [8,9] and possibly a cytotoxin [10]. Swine that recover from SD are immune to rechallenge [5,11] and parenteral vaccination against T.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the cell envelope of Treponema hyodysenteriae

Chatfield

Fernie

Beesley

et al. 1988

FEMS Microbiology Letters

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Cell envelope preparations of Treponema hyodysenteriae (strain CN 8368) were examined using biochemical and immunochemical methods. Several major polypeptides were detected with molecular weight between 24‐kDa and 45‐kDa. The majority of these polypeptides were recognised by serum from a pig vaccinated with an experimental whole‐cell T. hyodysenteriae vaccine and hyperimmune anti‐T. hyodysenteriae rabbit sera. Immune electron microscopy confirmed that the major antigens detected were associated with the cell envelope. Triton X‐100, in the presence of EDTA, completely solubilised a polypeptide with an approximate molecular weight of 36‐kDa. Antibodies to this polypeptide were not absorbed by whole T. innocens cells.

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Lipopolysaccharide in the pathogenicity of Treponema hyodysenteriae

Cited by 9 publications

References 61 publications

Consecutive pathological and immunological alterations during experimentally induced swine dysentery – A study performed by repeated endoscopy and biopsy samplings through an intestinal cannula

Consecutive pathological and immunological alterations during experimentally induced swine dysentery – A study performed by repeated endoscopy and biopsy samplings through an intestinal cannula

Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi

Characterisation of the cell envelope of Treponema hyodysenteriae

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