Lipopolysaccharide-binding protein: a potential marker of febrile urinary tract infection in childhood

Tsalkidou, Evanthia; Roilides, E; Gardikis, Stefanos; Trypsianis, Grigorios; Kortsaris, Alexandros; Chatzimichael, Athanassios; Tentes, Ioannis

doi:10.1007/s00467-013-2432-9

Cited by 16 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, LBP knockout mice are dramatically more susceptible to gram-negative infections compared with controls ( 43 ). Clinical studies have demonstrated that LBP is a useful biomarker for various infectious and infl ammatory states (44)(45)(46)(47).…”

Section: Cell Culturementioning

confidence: 99%

The macrophage LBP gene is an LXR target that promotes macrophage survival and atherosclerosis

Sallam

Ito

Rong

et al. 2014

Journal of Lipid Research

View full text Add to dashboard Cite

Section: Cell Culturementioning

confidence: 99%

The macrophage LBP gene is an LXR target that promotes macrophage survival and atherosclerosis

Sallam

Ito

Rong

et al. 2014

Journal of Lipid Research

View full text Add to dashboard Cite

“…The LBP binds to LPS and other microorganism components leading to activation of the inflammatory response [ 25 ]. LBP serum levels have been proposed as a useful diagnostic marker in both urinary infections in children and atherosclerosis, as well as a prognostic marker in acute appendicitis [ 26 ]-[ 28 ]. Future studies are warranted to confirm LBP as meaningful diagnostic marker in closed pyometra.…”

Section: Discussionmentioning

confidence: 99%

Molecular Expression Profile Reveals Potential Biomarkers and Therapeutic Targets in Canine Endometrial Lesions

et al. 2015

View full text Add to dashboard Cite

Cystic endometrial hyperplasia (CEH), mucometra, and pyometra are common uterine diseases in intact dogs, with pyometra being a life threatening disease. This study aimed to determine the gene expression profile of these lesions and potential biomarkers for closed-cervix pyometra, the most severe condition. Total RNA was extracted from 69 fresh endometrium samples collected from 21 healthy female dogs during diestrus, 16 CEH, 15 mucometra and 17 pyometra (eight open and nine closed-cervixes). Global gene expression was detected using the Affymetrix Canine Gene 1.0 ST Array. Unsupervised analysis revealed two clusters, one mainly composed of diestrus and CEH samples and the other by 12/15 mucometra and all pyometra samples. When comparing pyometra with other groups, 189 differentially expressed genes were detected. SLPI, PTGS2/COX2, MMP1, S100A8, S100A9 and IL8 were among the top up-regulated genes detected in pyometra, further confirmed by external expression data. Notably, a particular molecular profile in pyometra from animals previously treated with exogenous progesterone compounds was observed in comparison with pyometra from untreated dogs as well as with other groups irrespective of exogenous hormone treatment status. In addition to S100A8 and S100A9 genes, overexpression of the inflammatory cytokines IL1B, TNF and IL6 as well as LTF were detected in the pyometra from treated animals. Interestingly, closed pyometra was more frequently detected in treated dogs (64% versus 33%), with IL1B, TNF, LBP and CXCL10 among the most relevant overexpressed genes. This molecular signature associated with potential biomarkers and therapeutic targets, such as CXCL10 and COX2, should guide future clinical studies. Based on the gene expression profile we suggested that pyometra from progesterone treated dogs is a distinct molecular entity.

show abstract

“…The primary end point was evidence of microbial invasion evaluated after 5 days of supplementation and defined as 1 or more of the following: (i) positive conventional blood culture (blood cultures were read automatically using the BioMérieux BactAlert system [BioMérieux, Marcy L'Etoile, France]); (ii) evidence of microbial DNA in blood by polymerase chain reaction (PCR); (iii) plasma endotoxin level ≥0.5 endotoxin units/mL (50 pg/mL); or (iv) plasma level of lipopolysaccharide‐binding protein (LBP) ≥50 µg/mL . Blood samples were taken at the beginning of the trial, at the introduction of first enteral feeding, and when full enteral feeding was achieved (parenteral feeding was stopped when >75% of the patient's full nutrition requirement was tolerated enterally for a minimum of 24 hours).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Glutamine Supplementation on Microbial Invasion in Surgical Infants Requiring Parenteral Nutrition: Results of a Randomized Controlled Trial

Bishay

Simchowitz

Harris

et al. 2019

J Parenter Enteral Nutr

View full text Add to dashboard Cite

Background:To determine whether parenteral plus enteral glutamine supplementation influences microbial invasion in surgical infants who require parenteral nutrition (PN). Methods: An prospective double-blind randomized controlled trial studying surgical infants receiving PN for at least 5 days for congenital or acquired intestinal anomalies (2009-2012) was used. Infants were randomized to receive either glutamine supplementation (parenteral plus enteral; total 400 mg/kg/d) or isonitrogenous control. The primary end point was microbial invasion evaluated after 5 days of supplementation and defined as: (i) positive conventional blood culture, (ii) evidence of microbial DNA in blood (polymerase chain reaction), (iii) plasma endotoxin level ࣙ50 pg/mL, or (iv) plasma level of lipopolysaccharide binding protein ࣙ50 ng/mL. Data are given as median (range) and compared by logistic regression. Results: Sixty infants were randomized and reached the primary end point. Twenty-five patients had intestinal obstruction, 19 had abdominal wall defects, and 13 had necrotizing enterocolitis. Thirty-six infants showed evidence of microbial invasion during the study, and 17 of these were not detected by conventional blood culture. There was no significant difference between the 2 groups in the primary outcome; evidence of microbial invasion after 5 days was found in 9/31 (control group) and 8/29 (glutamine group) (odds ratio 0.83 [0.24-2.86; P = 0.77]). Conclusion: More than half of surgical infants requiring PN showed evidence of microbial invasion. Approximately half of this was not detectable by conventional blood cultures. Parenteral plus enteral glutamine supplementation had no effect on incidence of microbial invasion. (JPEN J Parenter Enteral Nutr. 2020;44:80-91)

show abstract

Lipopolysaccharide-binding protein: a potential marker of febrile urinary tract infection in childhood

Abstract: The results of this study suggest that the serum LBP concentration constitutes a reliable biologic marker for the diagnosis of a febrile UTI in children.

Cited by 16 publications

References 22 publications

The macrophage LBP gene is an LXR target that promotes macrophage survival and atherosclerosis

The macrophage LBP gene is an LXR target that promotes macrophage survival and atherosclerosis

Molecular Expression Profile Reveals Potential Biomarkers and Therapeutic Targets in Canine Endometrial Lesions

The Effect of Glutamine Supplementation on Microbial Invasion in Surgical Infants Requiring Parenteral Nutrition: Results of a Randomized Controlled Trial

Contact Info

Product

Resources

About