Lipopolysaccharide affinity for titanium implant biomaterials

Nelson, Steven K.; Knoernschild, Kent L.; Robinson, Fonda G.; Schuster, George S.

doi:10.1016/s0022-3913(97)70210-5

Cited by 43 publications

(42 citation statements)

References 27 publications

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“…17,18,22,25,27 Among the many other factors that can influence the results of particle incubation studies, the presence or absence of endotoxin may be especially important. Previous studies have illustrated that endotoxin can adhere to titanium surfaces 38 and can be detected on orthopedic implant surfaces as well as commercially available titanium-alloy particles. 31,32,39 Recent studies by Bi and co-workers, based on the incubation of relatively large, commercially prepared metal particles, suggested that pro-inflammatory cytokine release from macrophages in vitro actually may depend on endotoxin adsorbed onto the particles.…”

Section: Discussionmentioning

confidence: 99%

The effect of particle wear debris on NFκB activation and pro‐inflammatory cytokine release in differentiated THP‐1 cells

Akisue

Bauer

Farver

et al. 2001

J. Biomed. Mater. Res.

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Orthopedic wear debris has been thought to be an important factor associated with osteolysis and loosening of total joint arthroplasties. Previous in vitro studies have reported that particles of wear debris induce the release of pro-inflammatory cytokines and other inflammatory mediators from macrophages and other cells. Several recent investigations, however, have suggested that the wear particles themselves may not be primarily responsible for the inflammatory cellular responses, but that the observed cytokine release in vitro may be caused by endotoxin adsorbed to commercially available particle preparations. The intracellular pathways involved in macrophage signal transduction also are poorly understood. The purposes of this study are to use isolated orthopedic wear debris particles to evaluate pro-inflammatory cytokine release and nuclear factor kappa B (NFkappaB) activation from macrophages. Cells from human monocyte/macrophage cell line (THP-1) were differentiated and incubated with particles of debris that had been isolated from a failed human total hip arthroplasty. The titanium-alloy particles did not evoke release of TNF-alpha or IL-1beta whereas lipopolysaccharide (LPS) or LPS-treated debris particles induced both TNF-alpha and IL-1beta. LPS-treated particles, but not particles alone, stimulated NFkappaB activation. Our results suggest that at the concentrations tested in this study, endotoxin-free wear debris particles may not themselves initiate inflammatory cellular responses in differentiated THP-1 cells. It is unclear whether adsorbed endotoxin is clinically associated with osteolysis and/or loosening in total joint arthroplasties, but several factors, including adsorbed endotoxin, need to be investigated to explore the cellular responses responsible for osteolysis and/or loosening.

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Section: Discussionmentioning

confidence: 99%

The effect of particle wear debris on NFκB activation and pro‐inflammatory cytokine release in differentiated THP‐1 cells

Akisue

Bauer

Farver

et al. 2001

J. Biomed. Mater. Res.

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“…The best characterized bacterial debris, lipolysaccharide (LPS), is derived from Gram-negative bacteria and is resistant to extreme temperatures and pH values [30,31]. LPS is ubiquitous and has a high affinity for biomaterial surfaces [30,32,33]. It induces inflammatory pathophysiological responses by activating Toll-Like Receptor 4 (TLR-4), which is expressed by most mammalian cell types [34].…”

Section: Introductionmentioning

confidence: 99%

Surface contaminants inhibit osseointegration in a novel murine model

Bonsignore

Colbrunn

Tatro

et al. 2011

Bone

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Surface contaminants, such as bacterial debris and manufacturing residues, may remain on orthopaedic implants after sterilization procedures and affect osseointegration. The goals of this study were to develop a murine model of osseointegration in order to determine whether removing surface contaminants enhances osseointegration. To develop the murine model, titanium alloy implants were implanted into a unicortical pilot hole in the mid-diaphysis of the femur and osseointegration was measured over a five week time course. Histology, backscatter scanning electron microscopy and x-ray energy dispersive spectroscopy showed areas of bone in intimate physical contact with the implant, confirming osseointegration. Histomorphometric quantification of bone-to-implant contact and peri-implant bone and biomechanical pullout quantification of ultimate force, stiffness and work to failure increased significantly over time, also demonstrating successful osseointegration. We also found that a rigorous cleaning procedure significantly enhances bone-to-implant contact and biomechanical pullout measures by two-fold compared with implants that were autoclaved, as recommended by the manufacturer. The most likely interpretation of these results is that surface contaminants inhibit osseointegration. The results of this study justify the need for the development of better detection and removal techniques for contaminants on orthopaedic implants and other medical devices.

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“…This hypothesis is supported by the findings of studies that have demonstrated that bacterial endotoxin adheres to mineralized tissues, prostheses (8), orthodontic brackets (9) and implants (12).…”

mentioning

confidence: 63%

Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files

Silva¹,

Leonardo²,

Faccioli³

et al. 2007

Braz. J. Microbiol.

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This study evaluated the presence of lipopolysaccharide (LPS) in endodontic files after in vitro contamination with E. coli LPS and sterilization in dry heat or wet heat, with or without previous immersion in a Ca(OH)2 suspension. LPS was quantified using the Kinetic QCL TM test and data were analyzed statistically by the ttest. LPS was quantified only in the contaminated group, not submitted to any immersion or sterilization procedure (0.262±0.1296 endotoxin units/mL) (p=0.0003). In conclusion, both wet heat and dry heat sterilization were effective in inactivating LPS without the need of previous immersion of the files in a Ca(OH)2 suspension.

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Lipopolysaccharide affinity for titanium implant biomaterials

Cited by 43 publications

References 27 publications

The effect of particle wear debris on NFκB activation and pro‐inflammatory cytokine release in differentiated THP‐1 cells

The effect of particle wear debris on NFκB activation and pro‐inflammatory cytokine release in differentiated THP‐1 cells

Surface contaminants inhibit osseointegration in a novel murine model

Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files

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