Abstract:Products of the mitochondrial protein-synthesizing system have been labeled in vivo in the presence of cycloheximide in microaerobic cells and in cells from glucose-limited and glucose-repressed aerobic continuous cultures of Saccharomyces cerevisiae. Lipophilic proteins were extracted from labeled mitochondrial membranes with aqueous methanol and neutral and acidic chloroformmethanol solvents. In glucose-limited aerobic and microaerobic cells, about half of the total mitochondrial products were soluble in org… Show more
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