Lipopeptides from Bacillus sp. EA-CB0959: Active metabolites responsible for in vitro and in vivo control of Ralstonia solanacearum

Villegas-Escobar, Valeska; González-Jaramillo, Lina María; Ramírez, María C; Moncada, Ruth Natalia; Sierra-Zapata, Laura; Ordúz, Sergio; Romero-Tabarez, Magally

doi:10.1016/j.biocontrol.2018.06.005

Cited by 33 publications

(30 citation statements)

References 62 publications

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“…Similarly, in the operational group B. amyloliquefaciens, our results demonstrated that antibacterial activity was related to iturin and fengycin, as has been reported [46,47]. Strains IMA2, IMA6, IMA8 and IMA10 were able to produce iturin or both iturin and fengycin, corresponding to a higher antibacterial activity compared with IMA1 and IMA9 without lipopeptide production and with low antibacterial activity.…”

Section: Discussionsupporting

confidence: 85%

“…Similarly, there is no significant difference between the IMA2 and IMA8 in vivo assays, showing that iturin is the most important active ingredient. This is probably due to too little fengycin being secreted by IMA 8 under the same conditions, although few studies display that iturin and fengycin produced by B. amyloliquefaciens have high activity against R. solanacearum [46,47], and purified fengycin has stronger antibacterial activity than iturin [47]. By comparison, no lipopeptides are found in strains IMA 4, IMA 7 and IMA 11 except IMA4 but they also show higher disease control efficacy, possibly being associated with mechanisms of induced resistance [33,48].…”

Section: Discussionmentioning

confidence: 99%

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Isolation, Identification and Characterization of Rhizobacteria Strains for Biological Control of Bacterial Wilt (Ralstonia solanacearum) of Eggplant in China

Alamer

Tomah

et al. 2020

Agriculture

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Bacterial wilt of eggplant is the most destructive disease caused by Ralstonia solanacearum throughout the world. Eleven bacterial strains with high antagonistic activity were obtained from 245 rhizobacteria. Based on analysis of morphology, 16S rRNA sequences, fatty acid profiles, gyrA and rpoB genes, they were identified as Pseudomonas putida (IMA3), Paenibacillus polymyxa (IMA5), Bacillus cereus (IMA4, IMA7 and IMA11) and the “operational group Bacillus amyloliquefaciens” (IMA1, IMA2, IMA6, IMA8, IMA9 and IMA10). The lipopeptide compounds produced by each strain also were determined. The biocontrol tests demonstrated that co-inoculation by strain IMA5 and the pathogen gave the greatest biocontrol efficiency of 87.0% and 69.2% 30 and 40 days after co-inoculation, respectively. Plant growth promotion tests revealed that IMA5 markedly promoted eggplant growth, enhancing aboveground seedling length and biomass by 60.8% and by 107.6% and underground root length and biomass by 33.0% and 69.2%, respectively. Hence, strain IMA5 could be considered for developing potential biocontrol agents and for promoting plant growth characteristics, to aid the management of the pathogen R. solanacearum in eggplants.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Isolation, Identification and Characterization of Rhizobacteria Strains for Biological Control of Bacterial Wilt (Ralstonia solanacearum) of Eggplant in China

Alamer

Tomah

et al. 2020

Agriculture

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“…This study supported results showing the strong antibacterial activity of fengycin reported by Villegas‐Escobar et al . (). The contribution of iturin to the antibacterial activity of the LP mixture secreted by FJAT‐2349 was observed.…”

Section: Discussionmentioning

confidence: 97%

“…However, Villegas‐Escobar et al . () reported that iturin and fengycin produced by B. amyloliquefaciens were also highly active against R. solanacearum which causes Moko disease in banana plants. The purified fengycin from FJAT‐2349 could strongly inhibit the growth of R. solanacearum , which is responsible for the antibacterial activity of LP mixture.…”

Section: Discussionmentioning

confidence: 99%

“…The antibacterial activities of the LPs were determined by the agar disc diffusion method (Villegas‐Escobar et al . ). The R. solanacearum FJAT‐91 was cultured in liquid culture medium B at 30°C for 48 h. In brief, 0·5 ml of the bacterial culture suspensions was uniformly dispersed in the semisolid NA media at 40–50°C, and then poured in Petri plates (90 mm) coated with NA solid medium.…”

Section: Methodsmentioning

confidence: 97%

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Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

et al. 2019

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Aims The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT‐2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. Methods and Results Lipopeptides were extracted by acid precipitation and resolved in methanol and their structure was identified through LC‐QTOF‐MS/MS method. The antibacterial activities of the LPs were evaluated through inhibition zone experiments. The biocontrol efficiency of the LPs against tomato bacterial wilt was examined by a pot test. The LPs were composed of iturin (C14–C17 iturin A), fengycin (C14/C16 fengycin A, C14 fengycin B2, C16 fengycin A2/B2, C16–C17 fengycin B, C15 fengycin A derivatives and C15 fengycin B derivatives) and surfactin (C12–C16 surfactin A). Moreover, the composition of the LPs was significantly influenced by the culture medium and temperature; the contents of iturin, fengycin and surfactin varied within the range from 0·41–5·89, 4·54–181·67 and 2·05–19·65 mg l−1 in the different culture media and from 0·39–11·04, 1·45–215·14 and 7·79–24·43 mg l−1 under different culture temperatures respectively. The results indicated that the contents of the LP mixture, fengycin and surfactin secreted from FJAT‐2349 all decreased along with an increasing culture temperature. The fermentation supernatants and LP extracts had the strongest inhibition activities of Ralstonia solanacearum when strain FJAT‐2349 was incubated at 25°C using potato dextrose broth as the culture medium among all the assayed culture conditions. The purified fengycin was found to be the active antibacterial compound against R. solanacearum, but the purified surfactin was not. The pot experiments demonstrated that the LPs secreted from the strain FJAT‐2349 could effectively control the tomato bacterial wilt with a biocontrol efficiency of 97·6%. Conclusions The LPs secreted from strain FJAT‐2349 could serve as potential biocontrol agents against tomato bacterial wilt. Significance and Impact of the Study The LPs exhibited good potential applications in the biocontrol of tomato bacterial wilt.

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Characterization of a novel antifungal protein produced by Paenibacillus polymyxa isolated from the wheat rhizosphere

et al. 2020

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BACKGROUND Fusarium head blight (FHB) is one of the disasters that seriously harm wheat and other small grain crops. It causes spoilage and mildew of the grain leading to a significant decline in the yield and quality of the grain. This research aimed to isolate antagonistic bacteria to purify antifungal proteins. A strain was isolated from the rhizosphere of healthy wheat in a wheat field affected by a severe FHB epidemic. This isolated strain was tentatively identified as Paenibacillus polymyxa 7F1, which displayed a strong inhibitory effect against several other pathogens. One novel antifungal protein was purified from the P. polymyxa 7F1 and successfully expressed. RESULTS A crude culture of P. polymyxa 7F1 demonstrated antifungal activity that was stable at a temperature range of 60–90 °C and a pH range of 2.6–9.0. However, the antifungal activity of the P. polymyxa 7F1 was inhibited with proteinase K, trypsin, and neutral protease treatment. A 36 kDa protein with broad‐spectrum antifungal activity was purified from the P. polymyxa 7F1. A glycosyl hydrolase domain was identified from this protein through liquid chromatography–mass spectrometry (LC–MS) analysis. A recombinant plasmid pET32a(+)/36kd for prokaryotic expression was constructed, and the renatured p36kd protein demonstrated similar antifungal activity to the 36 kDa protein purified from the P. polymyxa 7F1. CONCLUSION A novel antifungal protein produced by P. polymyxa 7F1 was purified and expressed. The recombinant protein showed good antifungal activity as the novel purified protein. The novel antifungal protein provides an effective way to control the Fusarium head blight. © 2020 Society of Chemical Industry

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Lipopeptides from Bacillus sp. EA-CB0959: Active metabolites responsible for in vitro and in vivo control of Ralstonia solanacearum

Cited by 33 publications

References 62 publications

Isolation, Identification and Characterization of Rhizobacteria Strains for Biological Control of Bacterial Wilt (Ralstonia solanacearum) of Eggplant in China

Isolation, Identification and Characterization of Rhizobacteria Strains for Biological Control of Bacterial Wilt (Ralstonia solanacearum) of Eggplant in China

Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

Characterization of a novel antifungal protein produced by Paenibacillus polymyxa isolated from the wheat rhizosphere

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