Lipopeptides from a novel <i>Bacillus methylotrophicus</i> 39b strain suppress <i>Agrobacterium</i> crown gall tumours on tomato plants

Frikha‐Gargouri, Olfa; Abdallah, Dorra Ben; Ghorbel, Imen; Charfeddine, Ikram; Jlaiel, Lobna; Triki, Mohamed Ali; Tounsi, Slim

doi:10.1002/ps.4331

Cited by 46 publications

(36 citation statements)

References 37 publications

(75 reference statements)

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“…Another solution relies on partnering cultureindependent studies with culture-dependent ones, i.e., community analysis and characterization of isolates, where dominant or differential bacteria can be selectively isolated for functional verification in vitro (37,41,42). Some culturable strains of Rhizobium, Pseudomonas, Bacillus, and Pantoea are successful biocontrol agents (43,44) or have high biological control potential against crown gall disease (30,(45)(46)(47)(48)(49)(50)(51)(52). In this study, similar genera were found to be strongly associated with pathogen invasion in a resistant peach cultivar, and some strains showed antagonistic activity via in vitro tests (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Guo

et al. 2019

Appl Environ Microbiol

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Crown gall disease caused by Agrobacterium tumefaciens severely impacts the production of peach and other fruit trees. Several peach cultivars are partially resistant to A. tumefaciens, but little is known about the roles of endophytic microbiota in disease resistance. In the present study, the endophytic bacterial communities of resistant and susceptible peach cultivars "Honggengansutao" and "Okinawa" were analyzed using universal 16S rRNA gene amplicon sequencing in parallel with the cultivation and characterization of bacterial isolates. A total of 1,357,088 high-quality sequences representing 3,160 distinct operational taxonomic units (OTUs; Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes) and 1,200 isolates of 20 genera and 305 distinct ribotypes were collected from peach roots and twigs. It was found that factors including plant developmental stage, cultivar, and A. tumefaciens invasion strongly influenced the peach endophytic communities. The community diversity of endophytic bacteria and the abundance of culturable bacteria were both higher in the roots of the resistant cultivar, particularly after inoculation. Strikingly, the pathogen antagonists Streptomyces and Pseudomonas in roots and Rhizobium in twigs were most frequently detected in resistant plants. Our results suggest that the higher abundance and diversity of endophytic bacteria and increased proportions of antagonistic bacteria might contribute to the natural defense of the resistant cultivar against A. tumefaciens. This work reveals the relationships between endophytic bacteria and disease resistance in peach plants and provides important information for microbiome-based biocontrol of crown gall disease in fruit trees. IMPORTANCE Agrobacterium tumefaciens as the causal agent of peach crown gall disease can be controlled by planting resistant cultivars. This study profiles the endophytic bacteria in susceptible and resistant peach cultivars, advancing our understanding of the relationships between endophytic bacterial communities and peach crown gall disease, with potential implications for other complex microbiome-plantpathogen interactions. The resistant cultivar may defend itself by increasing the diversity and abundance of beneficial endophytic bacteria. The antagonists identified among the genera Streptomyces, Pseudomonas, and Rhizobium may have application potential for biocontrol of crown gall disease in fruit trees.

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Section: Discussionmentioning

confidence: 99%

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Guo

et al. 2019

Appl Environ Microbiol

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“…4 Recently, the Bacillus methylotrophicus 39d strain has been proposed as a biological agent for crown gall disease in tomato plants. 5 Chemical and agronomical methods for controlling crown gall disease are only partially effective. 3,6 Germplasm evaluation is the first step towards breeding for crown-gall-resistant plants.…”

Section: Introductionmentioning

confidence: 99%

Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot

Alburquerque

Faize

Burgos

2017

Pest Management Science

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“…Bacillus methylotrophicus, well-studied Gram-positive bacteria, has previously been identified as plant growth promoting [2]. Nevertheless, few reports describe the isolation and characterization of its secondary metabolites; they are mainly lipopeptides [3]. In the course of our studies to detect novel bioactive substances from the hot soil microorganisms [4], herein we report the isolation, structural elucidation, and antimicrobial activity of five natural products from B. methylotrophicus ASWU-C2.…”

Section: Introductionmentioning

confidence: 95%

Bacillus methylotrophicus ASWU-C2, a strain inhabiting hot desert soil, a new source for antibacterial bacillopyrone, pyrophen, and cyclopeptides

Helaly

Hamad

El‐Sayed

et al. 2018

Zeitschrift Für Naturforschung C

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A strain of Bacillus methylotrophicus was isolated from a soil sample collected in Aswan eastern desert, which is known for its extremely arid climate. After fermentation of the strain in liquid culture and subsequent extraction, a bioassay-guided isolation procedure yielded five compounds: 2-benzyl-4H-pyran-4-one, named bacillopyrone (1), pyrophen (2), macrolactin A (3) and the cyclopeptides malformin A1 (4), and bacillopeptin A (5). The structures were determined by interpretation of nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS) data. This is the first report on the isolation of compounds 1 and 2 from Bacillus species; compound 1 was reported previously as synthetic product. Bacillopyrone (1) exhibited moderate activity against the Gram-negative Chromobacterium violaceum with minimum inhibitory concentration 266.6 μg/mL, while macrolactin A (3) and malformin A1 (4) inhibited Staphylococcus aureus (minimum inhibitory concentrations 13.3 and 133.3 μg/mL, respectively).

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Lipopeptides from a novel Bacillus methylotrophicus 39b strain suppress Agrobacterium crown gall tumours on tomato plants

Cited by 46 publications

References 37 publications

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot

Bacillus methylotrophicus ASWU-C2, a strain inhabiting hot desert soil, a new source for antibacterial bacillopyrone, pyrophen, and cyclopeptides

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