Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway

Qi, Gaofu; Zhu, Fayin; Du, Peng; Yang, Xiufen; Qiu, Dewen; Zhou, Yu; Chen, Jingyuan; Zhao, Xiuyun

doi:10.1016/j.peptides.2010.08.003

Cited by 114 publications

(74 citation statements)

References 28 publications

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“…Two out of the 12 previously treated MBC patients in that study achieved a response [16]. Garufi et al [17] enrolled 14 patients with MBC pretreated with anthracycline in a phase II study of oxaliplatin at a dose of 130 mg/m 2 and with a dosing interval of 3 weeks. Three patients (21%) achieved a PR, 2 patients (14%) had stable disease and 9 patients (64%) showed progression.…”

Section: Experience With Single-agent Therapymentioning

confidence: 99%

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Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways

Luo¹,

Sun²,

Chan³

et al. 2011

Chemotherapy

124

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Background: Imperatorin (IM) is a furanocoumarin isolated from the root of Angelica dahurica, which is reported to have anticonvulsant and anticancer effects. In this study, the antiproliferative effect of IM on 9 human cancer cell lines was examined, and human hepatoma HepG2 cells were chosen as the target for preferential killing by IM. Particularly, the mechanism of IM-induced apoptosis and in vivo animal effects were also studied. Methods: Cell viability was measured using MTT assay, and apoptosis was detected by Hoechst staining, annexin V-PI staining, and DNA laddering assay. Mitochondrial membrane potential was detected by JC-1 staining. Western blot analysis was employed to detect the expression of apoptosis-related proteins. In addition, the in vivo anticancer effect of IM was examined in nude mice bearing HepG2 cells. Results: IM inhibited the proliferation of HepG2 cells through apoptosis induction in a time- and dose-dependent manner by observation of the nuclear morphology, DNA fragmentation, phosphatidylserine externalization, loss of mitochondrial membrane potential, release of cytochrome c into cytosol, and activation of caspase-3, caspase-8, caspase-9, and poly(ADP-ribose) polymerase cleavage. As cell death could partly be prevented by the caspase-8 or caspase-9 inhibitor and was evidenced by the results of Western blot analysis, our results also suggest that IM-induced apoptosis is mediated through both death receptor and mitochondrial pathways. In the animal model, IM was found to effectively suppress tumor growth by 31.93 and 63.18% at dosages of 50 and 100 mg/kg, respectively, after treatment for 14 days. No significant weight loss or toxicity to the hosts was found. Conclusions: IM can function as a cancer suppressor by inducing apoptosis in HepG2 cells through both death-receptor- and mitochondria-mediated pathways. Furthermore, the in vivo antitumor activities of IM are significant with negligible weight loss and damage to the host.

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Section: Experience With Single-agent Therapymentioning

confidence: 99%

“…Response lasted around 5 months, and median survival was 12 months. No severe toxicities were encountered [17]. …”

Section: Experience With Single-agent Therapymentioning

confidence: 99%

Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways

Luo¹,

Sun²,

Chan³

et al. 2011

Chemotherapy

124

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“…Bacillus amyloliquefaciens WH1-elaborated WH1fungin inhibited the growth of Rhizoctonia solani, Helminthosprium maydis, Fusarium oxysporium, Botrytis cinereapers, Gibberella saubinetii, Colletotrichum gossypii, C. capsici, Physalospora piricola, Sclerotinia sclerotiorum and Candida albicans (Qi et al 2010;De Brucker et al 2011). At low dose, WH1 fungin impeded glucan synthesis and ATPase activity.…”

Section: Antifungalmentioning

confidence: 99%

“…Surfactin with a hydrophobic pentadecanoic fatty acyl chain was characterized to possess the hemolytic activity (Deleu et al 2003). WH1fungin is a type of surfactin elaborated by Bacillus amyloliquefaciens WH1 (Qi et al 2010). This lipopeptide demonstrated amelioration of type 1 diabetes mellitus in mouse model by immunomodulation ).…”

Section: Surfactinmentioning

confidence: 99%

Therapeutic cyclic lipopeptides mining from microbes: latest strides and hurdles

Patel

Ahmed

Eswari

2015

World J Microbiol Biotechnol

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Infectious diseases impose serious public health burdens and often have devastating consequences. The cyclic lipopeptides elaborated by bacteria Bacillus, Paenibacillus, Pseudomonas, Streptomyces, Serratia, Propionibacterium and fungus Fusarium are very crucial in restraining the pathogens. Composed of a peptide and a fatty acyl moiety these amphiphilic metabolites exhibit broad spectrum antimicrobial effects. Among the plethora of cyclic lipopeptides, only selective few have emerged as robust antibiotics. For their functional vigor, polymyxin, daptomycin, surfactin, iturin, fengysin, paenibacterin and pseudofactin have been integrated in mainstream healthcare. Daptomycin has been a significant part of antimicrobial arsenal since the past decade. As the magnitude of drug resistance rises in unprecedented manner, the urgency of prospecting novel cyclic lipopeptides is being perceived. Intense research has revealed the implication of these bioactive compounds stretching beyond antibacterial and antifungal. Anticancer, immunomodulatory, prosthetic parts disinfection and vaccine adjuvancy are some of the validated prospects. This review discusses the emerging applications, mechanisms governing the biological actions, role of genomics in refining structure and function, semi-synthetic analog discovery, novel strain isolation, setbacks etc. Though its beyond the scope of the current topic, for holistic purpose, the role of lipopeptides in bioremediation and crop biotechnology has been briefly outlined. This updated critique is expected to galvanize innovations and diversify therapeutic recruitment of microbial lipopeptides.

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“…Surfactin (C15) was purified from Bacillus amyloliquefaciens WH1 culture as described previously (Qi et al, 2010). Recombinant human INS was a gift from JS Bioway Pharmaceutical Co., Ltd (Changzhou, China).…”

Section: Materials and Reagentsmentioning

confidence: 99%

A natural lipopeptide of surfactin for oral delivery of insulin

et al. 2016

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Surfactin, a natural lipopeptide produced by Bacillus, is gaining attention for potentially biomedical and pharmaceutical applications. Here, surfactin was assayed for oral delivery of insulin (INS) by its ability to bind to and promote protein to penetrate through the cell membrane. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, surfactin was found to form co-precipitates with INS to protect it from acidic and enzymatic attack in the gastrointestinal tract. Further analysis by non-reductive electrophoresis showed surfactin could bind to INS forming heteropolymers. Analysis with circular dichroism, we found this binding significantly influenced the INS structure with decreased rigid a-helix and b-turn, but with increased flexible b-sheet and random coil. The change with more flexible structure was favorable for INS to penetrate through the cell membrane. Fluorescence spectra analysis also showed surfactin could lead Phe and Tyr in the inner of INS exposed outside, further promoting INS permeabilization by improving the hydrophobic-lipophilic interactions between INS and cell membrane. As a result, the effective permeability (Peff) of INS plus surfactin was 4.3 times of that of INS alone. In vivo assay showed oral INS with surfactin displayed excellent hypoglycemic effects with a relative bioavailability of 12.48% and 5.97% in diabetic mice and non-diabetic dogs, respectively. Summary, surfactin is potential for oral delivery of INS by its role as an effective protease inhibitor and permeability enhancer.

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Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway

Cited by 114 publications

References 28 publications

Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways

Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways

Therapeutic cyclic lipopeptides mining from microbes: latest strides and hurdles

A natural lipopeptide of surfactin for oral delivery of insulin

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