Lipooligosaccharide locus classes are associated with certain Campylobacter jejuni multilocus sequence types

Revez, Joana; Hänninen, Marja‐Liisa

doi:10.1007/s10096-012-1556-3

Cited by 20 publications

(20 citation statements)

References 21 publications

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“…Overall, the ST-45 CC was positive for GGT and ansB(s) and negative for fucP, but ST-45 could be divided into three groups: GGT and ansB(s) positive, ansB(s) positive only, and negative for all traits. The observed heterogeneity among isolates of ST-45 was found previously in a variety of studies using Penner heat-stable serotypes (6), FlaA short-variable-region variants (5), microarrays (37), stress response analyses (13), and lipooligosaccharide locus class distributions (20,30) and is not surprising, as ST-45 has been isolated from a wide variety of sources (21; http://pubmlst.org /campylobacter/) and seems to adapt easily to new niches. In contrast to ST-45, ST-50 (ST-21 CC) retained solely fucP.…”

Section: Discussionsupporting

confidence: 58%

Association of Campylobacter jejuni Metabolic Traits with Multilocus Sequence Types

Haan

Llarena

Revez

et al. 2012

Appl Environ Microbiol

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In this study, we describe the association of three Campylobacter jejuni metabolism-related traits, ␥-glutamyl-transpeptidase (GGT), fucose permease (fucP), and secreted L-asparaginase [ansB(s)], with multilocus sequence types (STs). A total of 710 C. jejuni isolates with known STs were selected and originated from humans, poultry, bovines, and the environment. Among these isolates, we found 31.1% to produce GGT and 49.3% and 30.3% to be positive for ansB(s) and fucP, respectively. The combination of GGT production, the presence of ansB(s), and the absence of fucP was associated with ST-22, ST-586, and the ST-45 and ST-283 clonal complexes (CCs), which were the main STs and CCs found among the human and chicken isolates. The ST-21 CC was associated with the presence of fucP and was the major CC among the bovine isolates. Although the ST-61 CC was the second major CC among the bovine isolates, these isolates did not have any of the markers studied, making the role of fucP in bovine gut colonization questionable. The ST-45 CC was subdivided into three groups that were attributed solely to ST-45. One group showed a marker combination described previously, another group was found to be positive for ansB(s) only, and the third group did not have any of the markers studied. These results suggest that the host association of these markers seems to be indirect and may arise as a consequence of host-ST and -CC associations. Thus, a representative collection of STs should be tested to draw sensible conclusions in similar studies.

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Section: Discussionsupporting

confidence: 58%

Association of Campylobacter jejuni Metabolic Traits with Multilocus Sequence Types

Haan

Llarena

Revez

et al. 2012

Appl Environ Microbiol

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“…Within this CC, the founder ST-45 is very heterogeneous by Penner heat-stable serotyping (Dingle et al , 2001), flaA short-variable-region typing (Dingle et al , 2002), comparative genomic hybridization (Taboada et al , 2008), stress response analyses (Habib et al , 2009), lipooligosaccharide locus class distributions (Revez & Hänninen, 2012), and whole-genome MLST (Kovanen et al , 2016). The existence of several possible animal host species, lack of host signals and genetic and phenotypic heterogeneity of this CC complicate the use of WGS in epidemiological investigations and source attribution.…”

Section: Introductionmentioning

confidence: 99%

Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion

et al. 2016

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The decreased costs of genome sequencing have increased the capability to apply whole-genome sequencing to epidemiological surveillance of zoonotic Campylobacter jejuni. However, knowledge of the genetic diversity of this bacteria is vital for inferring relatedness between epidemiologically linked isolates and a necessary prerequisite for correct application of this methodology. To address this issue in C. jejuni we investigated the spatial and temporal signals in the genomes of a major clonal complex and generalist lineage, ST-45 CC, by analysing the population structure and genealogy as well as applying genome-wide association analysis of 340 isolates from across Europe collected over a wide time range. The occurrence and strength of the geographical signal varied between sublineages and followed the clonal frame when present, while no evidence of a temporal signal was found. Certain sublineages of ST-45 formed discrete and genetically isolated clades containing isolates with extremely similar genomes regardless of time and location of sampling. Based on a separate data set, these monomorphic genotypes represent successful C. jejuni clones, possibly spread around the globe by rapid animal (migrating birds), food or human movement. In addition, we observed an incongruence between the genealogy of the strains and multilocus sequence typing (MLST), challenging the existing clonal complex definition and the use of whole-genome gene-by-gene hierarchical nomenclature schemes for C. jejuni.

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“…Valuable insights into the genetic origins of significant strainvariable traits have been gained by studying the effects of C. jejuni LOS genotypes on phenotype (19)(20)(21)(22)(23)(24). However, so far, only two studies have addressed the variation in gene composition of the C. coli LOS biosynthesis locus.…”

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confidence: 99%

Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

et al. 2016

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Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity. After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity. Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni. 2-Amino-2-deoxy-D-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the ␤-1=-6-linked 3-diamino-2,3-dideoxy-D-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni. Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-D-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates. Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. IMPORTANCEDespite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs. Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information. We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. C ampylobacteriosis is the most common bacterial foodborne disease in developed countries, with over 200,000 human cases being reported annually in the European Union alone (1). The true burden of the disease in the population is likely underestimated, as many infections result in mild gastroenteritis (1). Approximately 80% of reported infections are caused by Campylobacter jejuni, and 7 to 18% of cases are attributed to C. coli. Therefore, C. coli is among the five most important bacterial etiological agents of human gastroenteritis (2, 3).As in other Gram-negative bacteria, Campylobacter species cell surface glycoconjugates, including lipooligosaccharides (LOSs), play an important role in serum and bile resistance; resistanc...

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Lipooligosaccharide locus classes are associated with certain Campylobacter jejuni multilocus sequence types

Cited by 20 publications

References 21 publications

Association of Campylobacter jejuni Metabolic Traits with Multilocus Sequence Types

Association of Campylobacter jejuni Metabolic Traits with Multilocus Sequence Types

Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion

Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

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