Lipooligosaccharide epitopes shared among Gram-negative non-enteric mucosal pathogens

Campagnari, Anthony A.; Spinola, Stanley M.; Lesse, Alan J.; Kwaik, Yousef Abu; Mandrell, Robert E.; Apicella, Michael A.

doi:10.1016/0882-4010(90)90094-7

Cited by 106 publications

(75 citation statements)

References 20 publications

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“…This form of molecular mimicry exhibited by M. catarrhalis may provide the pathogen with an advantage for evading host defence mechanisms. This structural feature also occurs in LPS of pathogenic strains of Haemophilus injluenzae (24,25) and Neisseria spp. (25) and it has been implicated in virulence potential (26).…”

Section: +6)-p-d-glcpn-(l+6)-d-glcnol Unit Derived From the Lipid A Rmentioning

confidence: 99%

“…This structural feature also occurs in LPS of pathogenic strains of Haemophilus injluenzae (24,25) and Neisseria spp. (25) and it has been implicated in virulence potential (26). Concurrent studies on the LPS produced by clinical isolates of M. catarrhalis indicate that the OS structure deduced in the present investigation is common to LPS produced by these strains.…”

Section: +6)-p-d-glcpn-(l+6)-d-glcnol Unit Derived From the Lipid A Rmentioning

confidence: 99%

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Structural elucidation of the backbone oligosaccharide from the lipopolysaccharide of Moraxella catarrhalis serotype A

Masoud¹,

Perry²,

Brisson³

et al. 1994

Can. J. Chem.

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. Can. J. Chem. 72, 1466Chem. 72, (1994.The cell-surface lipopolysaccharide produced by Mornxella catarrhalis serotype A is composed of a hydrophobic lipid A moiety and an oligosaccharide, but lacks high-molecular-weight 0-polysaccharide chains. The oligosaccharide component is composed of D-glucose, D-galactose, D-glucosamine, and 3-deoxy-D-manno-octulosonic acid. The carbohydrate backbone was obtained from the lipopolysaccharide by employing a reaction sequence involving deacylation, dephosphorylation, and reduction of the reducing D-glucosamine terminus of the lipid A moiety. Structural analysis of the backbone oligosaccharide employed a combination of microanalytical methods and nuclear magnetic resonance spectroscopy. Homo-and hetero-nuclear chemical shift correlation techniques and nuclear Overhauser enhancement (NOE) experiments led to the unambiguous assignment of the [Traduit par la Redaction]

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Section: +6)-p-d-glcpn-(l+6)-d-glcnol Unit Derived From the Lipid A Rmentioning

confidence: 99%

Section: +6)-p-d-glcpn-(l+6)-d-glcnol Unit Derived From the Lipid A Rmentioning

confidence: 99%

Structural elucidation of the backbone oligosaccharide from the lipopolysaccharide of Moraxella catarrhalis serotype A

Masoud¹,

Perry²,

Brisson³

et al. 1994

Can. J. Chem.

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“…For immuno-TEM, samples were labeled overnight with an anti-NTHi monoclonal antibody, 6E4, followed by goat anti-mouse IgG ultra-small gold secondary antibody labeling (Electron Microscopy Sciences, Hatfield, PA) and silver enhancement. The 6E4 antibody recognizes an NTHi lipooligosaccharide (LOS) epitope and is specific for H. influenzae LOS (18). Controls for immuno-TEM included the following: no primary antibody on LRW-embedded CF BALF; LRW-embedded NTHi 2019 for a positive control; and LRWembedded 10 7 cfu NTHi 2019 with 10 5 primary human airway epithelial cells for a centrifugation artifact control.…”

Section: Human Bal Samplesmentioning

confidence: 99%

Haemophilus influenzaeForms Biofilms on Airway Epithelia

Starner

Zhang

Kim

et al. 2006

Am J Respir Crit Care Med

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155

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“…In a recent study, an isogenic heptosyltransferase-III knockout strain (35000hepϪ) was constructed by Filiatrault et al [20] in which immunochemical and molecular mass data suggested the presence of several unusual structural features. Specifically, a series of high molecular weight glycoforms (M r Ͼ 3000) were observed by SDS-PAGE which reacted with a monoclonal antibody (3F11) that recognizes LOS containing terminal LacNAc or paragloboside [21]. Moreover, the masses of the glycoforms as determined by MALDI-MS indicated that these glycoforms were formed by mutiple additions of alternating Hex and HexNAc residues, consistent with a polyLacNAc structure.…”

mentioning

confidence: 95%

Characterization of lipooligosaccharides from Haemophilus ducreyi containing polylactosamine repeats

Schilling

Gibson

Filiatrault

et al. 2002

J. Am. Soc. Mass Spectrom.

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Haemophilus ducreyi, a gram-negative human mucosal pathogen, is one of the principal causes of genital ulcer disease. The lipooligosaccharides (LOS) of these bacteria are considered to be a major virulence factor and have been implicated in the adherence and invasion of H. ducreyi to several human cell types. An isogenic heptosyltransferase-III knockout strain (waaQ) was recently constructed from H. ducreyi 35000 wild-type strain and immunochemical and molecular weight data of the isolated LOS suggested the presence of poly-N-acetyllactosamine (LacNAc) (Filiatrault et al., Infect. Immun. 2000, 68, 3352-3361). In this present study, the structures of these novel LOS-glycoforms were characterized by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry in combination with exoglycosidase digestion. Detailed structural information was obtained for the oligosaccharide (OS) portions of these LOS showing between one to five linear LacNAc repeats on the non-reducing terminus of the main oligosaccharide branch. When grown on solid media, the organism produced LacNAc repeats that were further modified by the addition of sialic acid. Enzymatic digestion with ␤-galactosidase, ␤-N-acetylhexosaminidase, and neuraminidase type VI-A yielded truncated glycoforms consistent with a polyLacNAc structure capped at various end points with sialic acid. ESI-MS/MS mass spectrometry on a quadrupole time-offlight instrument was particularly effective in obtaining detailed structural information on the least abundant, high-mass glycoforms. Although LOS containing terminal di-LacNAc have been reported, this is the first time to our knowledge that a linear polyLacNAc structure has been characterized in bacteria. (J Am Soc Mass Spectrom 2002, 13, 724 -734)

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Lipooligosaccharide epitopes shared among Gram-negative non-enteric mucosal pathogens

Cited by 106 publications

References 20 publications

Structural elucidation of the backbone oligosaccharide from the lipopolysaccharide of Moraxella catarrhalis serotype A

Structural elucidation of the backbone oligosaccharide from the lipopolysaccharide of Moraxella catarrhalis serotype A

Haemophilus influenzaeForms Biofilms on Airway Epithelia

Characterization of lipooligosaccharides from Haemophilus ducreyi containing polylactosamine repeats

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