Lipolysis stimulating peptides of potato protein hydrolysate effectively suppresses high-fat-diet-induced hepatocyte apoptosis and fibrosis in aging rats

Abstract

BackgroundNon-alcoholic fatty liver disease (NAFLD) is one of the most common outcomes of obesity and is characterized by the accumulation of triglycerides, increased tissue apoptosis, and fibrosis. NAFLD is more common among elderly than in younger age groups, and it causes serious hepatic complications.ObjectiveIn this study, alcalase treatment derived potato protein hydrolysate (APPH) with lipolysis-stimulating property has been evaluated for its efficiency to provide hepato-protection in a high-fat-diet (HFD)-fed aging rats.DesignTwenty-four-month-old SD rats were randomly divided into six groups (n=8): aged rats fed with standard chow, HFD-induced aged obese rats, HFD with low-dose (15 mg/kg/day) APPH treatment, HFD with moderate (45 mg/kg/day) APPH treatment, HFD with high (75 mg/kg/day) APPH treatment, and HFD with probucol.ResultsAPPH was found to reduce the NAFLD-related effects in rat livers induced by HFD and all of the HFD-fed rats exhibited heavier body weight than those with control chow diet. However, the HFD-induced hepatic fat accumulation was effectively attenuated in rats administered with low (15 mg/kg/day), moderate (45 mg/kg/day), and high (75 mg/kg/day) doses of APPH. APPH oral administration also suppressed the hepatic apoptosis- and fibrosis-related proteins induced by HFD.ConclusionsOur results thus indicate that APPH potentially attenuates hepatic lipid accumulation and anti-apoptosis and fibrosis effects in HFD-induced rats. APPH may have therapeutic potential in the amelioration of NAFLD liver damage.

Andrographolide mitigates cardiac apoptosis to provide cardio‐protection in high‐fat‐diet‐induced obese mice

“…In this study, the C57/BL6 mice showed increase in the IGF‐1R survival mechanism as a compensative protective mechanism against HFD and andrographolide treatment further upregulated the IGF‐1R survival mechanism and fortified the cardio‐protection in obese mice (Figure ). Furthermore, our previous studies on obesity and diabetes in Zuker rats, SD rats, C57/BL6 mice and in hamsters showed a common set of changes in cardiac tissue morphology such as cardiac tissue disarray, increased interstitial space, and minor cardiac fibrosis, and further displayed significantly increased protein levels of Fas ligand, Fas death receptors, and FADD . In this study, HFD feeding in C57/BL6 mice triggered increase in the number of TUNEL‐positive apoptotic cells in heart and further caused minor cardiac fibrosis.…”

Bioactive Peptide Improves Diet-Induced Hepatic Fat Deposition and Hepatocyte Proinflammatory Response in SAMP8 Ageing Mice

“…For instance, administration of Black soy peptides (BSP, 10%) was able to restore the decreased phospho-AMPK level in white adipose tissue (WAT) of diet-induced obese (DIO) mice fed a HFD [21]. Therefore, considering that HFD-induced inflammation in liver can impair AMPK function related to cellular metabolic homeostasis, we sought to investigate whether our already-tested lipolysis-activating PH and its derived bioactive peptide [5], by interfering with HFD-driven proinflammatory response, could restore the activity of molecular regulators involving in hepatic lipid metabolism; thus would alleviate NAFLD burden in aging model. On this basis, we have designed a fourteen-week experimental study with SAM8 mice feeding a normal chow diet (NC) or a HFD and then without (HCO) or with simultaneous administration of peptide (HPEP), protein hydrolysate (HPH) or probucol, a hypocholesterolaemic drug (HRX).…”

“…Hepatosteatosis, the early reversible stage of NAFLD, may progress to nonalcoholic steatohepatitis (NASH) and furtherly to fibrosis, cirrhosis and hepatocellular carcinoma (HCC) [2,3], with subsequent health and social burden [4]. To study fatty liver and potential therapeutic strategy our lab has successfully developed various dietary murine models and investigated protein hydrolysates from soy or potato with beneficial therapeutic effects [5][6][7]. Considering special issues with drug administration in elderly, we purposed in this present work to induce NAFLD in SAMP8 specific strains with HFD and evaluate our natural ingredients.…”

Oral administration of alcalase potato protein hydrolysate‐APPH attenuates high fat diet‐induced cardiac complications via TGF‐β/GSN axis in aging rats

“…The protective effect of APPH could be because of the lipolysis‐stimulating property. APPH was demonstrated to possess fragments with lipolysis activating peptides like patatin in animal models …”