Lipofibroblasts in Structurally Normal, Fibrotic, and Emphysematous Human Lungs

Schipke, Julia; Kuhlmann, Susanne; Hegermann, Jan; Fassbender, Susanne; Kühnel, Mark Philipp; Jonigk, Danny; Mühlfeld, Christian

doi:10.1164/rccm.202101-0043le

Cited by 15 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We further found that the most sensitive FIB subpopulation in O. niloticus gills was LPFs, which are a crucial subtype of FIBs responsible for structural support and cell−cell communication. 54 However, there are still some limitations for the proposed FIB cell-type biomarker. Currently, we assume that this biomarker is conservatively suitable for specific fish species, tissues, MP type, and MP size employed in this study.…”

Section: ■ Discussionmentioning

confidence: 99%

Single-Cell RNA Sequencing Profiling Cellular Heterogeneity and Specific Responses of Fish Gills to Microplastics and Nanoplastics

Zheng,

Wang

2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

Fish gills are highly sensitive organs for microplastic (MP) and nanoplastic (NP) invasions, but the cellular heterogeneity of fish gills to MPs and NPs remains largely unknown. We employed single-cell RNA sequencing to investigate the responses of individual cell populations in tilapia Oreochromis niloticus gills to MP and NP exposure at an environmentally relevant concentration. Based on the detected differentially expressed gene (DEG) numbers, the most affected immune cells by MP exposure were macrophages, while the stimulus of NPs primarily targeted T cells. In response to MPs and NPs, H + -ATPase-rich cells exhibited distinct changes as compared with Na + /K + -ATPase-rich cells and pavement cells.Fibroblasts were identified as a potential sensitive cell-type biomarker for MP interaction with O. niloticus gills, as evidenced by the largely reduced cell counts and the mostly detected DEGs among the 12 identified cell populations. The most MP-sensitive fibroblast subpopulation in O. niloticus gills was lipofibroblasts. Cell−cell communications between fibroblasts and H + -ATPase-rich cells, neurons, macrophages, neuroepithelial cells, and Na + /K + -ATPase-rich cells in O. niloticus gills were significantly inhibited by MP exposure. Collectively, our study demonstrated the cellular heterogeneity of O. niloticus gills to MPs and NPs and provided sensitive markers for their toxicological mechanisms at single-cell resolution.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Single-Cell RNA Sequencing Profiling Cellular Heterogeneity and Specific Responses of Fish Gills to Microplastics and Nanoplastics

Zheng,

Wang

2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…The ability of lungs to accumulate retinoids locally has long been attributed to the pulmonary lipofibroblasts, lipid-laden interstitial cells located in the alveolar niche between alveolar capillary endothelial cells and the alveolar epithelial cells present in both murine 32 , 63 , 64 , 100 and human lungs 83 , 101 , 102 (Supplementary Fig. 1b ).…”

Section: Discussionmentioning

confidence: 99%

Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice

et al. 2023

View full text Add to dashboard Cite

Retinoids are potent transcriptional regulators that act in regulating cell proliferation, differentiation, and other cellular processes. We carried out studies in male mice to establish the importance of local cellular retinoid stores within the lung alveolus for maintaining its health in the face of an acute inflammatory challenge induced by intranasal instillation of lipopolysaccharide. We also undertook single cell RNA sequencing and bioinformatic analyses to identify roles for different alveolar cell populations involved in mediating these retinoid-dependent responses. Here we show that local retinoid stores and uncompromised metabolism and signaling within the lung are required to lessen the severity of an acute inflammatory challenge. Unexpectedly, our data also establish that alveolar cells other than lipofibroblasts, specifically microvascular endothelial and alveolar epithelial cells, are able to take up lipoprotein-transported retinoid and to accumulate cellular retinoid stores that are directly used to respond to an acute inflammatory challenge.

show abstract

“…Within the septa, several cell types fulfill lipid-related tasks, i.e. alveolar epithelial type 2 (AT2) cells (production and modification of complex lipid species for surfactant synthesis), interstitial fibroblasts (lipid storage) and alveolar macrophages (lipid recycling and breakdown) [ 53 , 54 , 55 ]. Since individual cell types were not addressed in this study, cell type-specific alterations were possibly not detected.…”

Section: Discussionmentioning

confidence: 99%

Oral Supplementation with the Polyamine Spermidine Affects Hepatic but Not Pulmonary Lipid Metabolism in Lean but Not Obese Mice

Pankoke

Pfarrer²,

Glage³

et al. 2022

Nutrients

View full text Add to dashboard Cite

The polyamine spermidine is discussed as a caloric restriction mimetic and therapeutic option for obesity and related comorbidities. This study tested oral spermidine supplementation with regard to the systemic, hepatic and pulmonary lipid metabolism under different diet conditions. Male C57BL/6 mice were fed a purified control (CD), high sucrose (HSD) or high fat (HFD) diet with (-S) or without spermidine for 30 weeks. In CD-fed mice, spermidine decreased body and adipose tissue weights and reduced hepatic lipid content. The HSD induced hepatic lipid synthesis and accumulation and hypercholesterolemia. This was not affected by spermidine supplementation, but body weight and blood glucose were lower in HSD-S compared to HSD. HFD-fed mice showed higher body and fat depot weights, prediabetes, hypercholesterolemia and severe liver steatosis, which were not altered by spermidine. Within the liver, spermidine diminished hepatic expression of lipogenic transcription factors SREBF1 and 2 under HSD and HFD and affected the expression of other lipid-related enzymes. In contrast, diet and spermidine exerted only minor effects on pulmonary parameters. Thus, oral spermidine supplementation affects lipid metabolism in a diet-dependent manner, with significant reductions in body fat and weight under physiological nutrition and positive effects on weight and blood glucose under high sucrose intake, but no impact on dietary fat-related parameters.

show abstract

Lipofibroblasts in Structurally Normal, Fibrotic, and Emphysematous Human Lungs

Cited by 15 publications

References 10 publications

Single-Cell RNA Sequencing Profiling Cellular Heterogeneity and Specific Responses of Fish Gills to Microplastics and Nanoplastics

Single-Cell RNA Sequencing Profiling Cellular Heterogeneity and Specific Responses of Fish Gills to Microplastics and Nanoplastics

Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice

Oral Supplementation with the Polyamine Spermidine Affects Hepatic but Not Pulmonary Lipid Metabolism in Lean but Not Obese Mice

Contact Info

Product

Resources

About