Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity

Tariq, Imran; Pinnapireddy, Shashank Reddy; Duse, Lili; Ali, Muhammad Yasir; Ali, Sajid; Amin, Muhammad; Goergen, Nathalie; Jedelská, Jarmila; Schäfer, Jens; Bakowsky, Udo

doi:10.1016/j.ejpb.2018.12.013

Cited by 32 publications

(21 citation statements)

References 43 publications

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“…The next day, cells were washed with PBS containing Ca 2+ and Mg 2+ (pH 7.4) and were incubated for 30 min with pharmacological inhibitors of different endocytic mechanisms viz. dynasore (80 µM), chlorpromazine (14 µM) and Filipin III (8 mM) [40]. After incubation with the uptake inhibitors, cells were washed again with PBS and exposed to 50 µM CUR-NP or free curcumin (dissolved in DMSO) for 4 h. After irradiation at 457 nm with a radiation fluence of 8.6 J/cm 2 , cells were incubated with MTT and the absorbance from resulting formazan crystals (dissolved in DMSO) was determined at 570 nm as described above.…”

Section: Methodsmentioning

confidence: 99%

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

et al. 2019

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Accumulation of photosensitisers in photodynamic therapy in healthy tissues is often the cause of unwanted side effects. Using nanoparticles, improved bioavailability and site-specific drug uptake can be achieved. In this study, curcumin, a natural product with anticancer properties, albeit with poor aqueous solubility, was encapsulated in biodegradable polymeric poly(lactic-co-glycolic acid) (PLGA) nanoparticles (CUR-NP). Dynamic light scattering, laser Doppler anemometry and atomic force microscopy were used to characterise the formulations. Using haemolysis, serum stability and activated partial thromboplastin time tests, the biocompatibility of CUR-NP was assessed. Particle uptake and accumulation were determined by confocal laser scanning microscopy. Therapeutic efficacy of the formulation was tested in SK-OV-3 human ovarian adenocarcinoma cells post low level LED irradiation by determining the generation of reactive oxygen species and cytotoxicity. Pharmacologic inhibitors of cellular uptake pathways were used to identify the particle uptake mechanism. CUR-NP exhibited better physicochemical properties such as stability in the presence of light and improved serum stability compared to free curcumin. In addition, the novel nanoformulation facilitated the use of higher amounts of curcumin and showed strong apoptotic effects on tumour cells.

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Section: Methodsmentioning

confidence: 99%

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

et al. 2019

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“…393,394 In non-viral gene delivery, lipids are currently the gold standard. For example, liposomes were applied as DNA and RNA nanocarriers 395,396 as well as mimics of the viral envelope for gene delivery. 397 Recent reviews on non-viral application of lipids for gene delivery can be found elsewhere.…”

Section: Lipidsmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“…The polymer condenses with DNA to form a positively charged nanoparticle. [ 33,34 ] The lipopolyplexes (LPP) at different mass ratio were prepared by adding ultrasound contrast vesicles into polyplexes. The average size of complex, formed with electrostatic interaction between positively charged polyplex and negatively charged lipid vesicles, was measured by photon correlation spectroscopy.…”

Section: Resultsmentioning

confidence: 99%

“…The average size was found to be not more than 500 nm at mass ratio 0.6 and 0.7, which indicate the initiation of aggregation of lipopolyplexes. [ 33 ] The zeta potentials were found to decrease up to 3 mV on successive addition of anionic lipid vesicles (Figure 3a). Notably, the particle size of both lipopolyplexes formulations was larger than the sizes of initial ultrasound contrast vesicles.…”

Section: Resultsmentioning

confidence: 99%

Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model

Shah

Tariq

Engelhardt

et al. 2020

Macromolecular Bioscience

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This work focuses on the development of ultrasound contrast vesicles for ultrasound‐mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equivalent to the commercially available SonoVue. These anionic lipid vesicles establish electrostatic interaction with cationic polyplexes based on linear polyethylenimine (22kDa) forming lipopolyplexes with ultrasound contrast. The lipopolyplexes are characterized regarding shape, size, and zeta potential. When exposed to low frequency ultrasound, these carriers show elevated transfection efficiency and reduced cytotoxicity. The effect of post‐transfection ultrasound on cellular uptake of lipopolyplexes is also evaluated. An analogous transfection is also observed in the tumor mimicking multicellular 3D spheroid culture of ovarian cancer cells. The emergence of tumor imaging and enhanced gene delivery by medical ultrasound, a noninvasive imaging modality, is considered paving the way for efficient theranostic gene therapy.

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Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity

Cited by 32 publications

References 43 publications

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

Materials promoting viral gene delivery

Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model

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