Lipoaspirate fluid proteome: A preliminary investigation by LC-MS top-down/bottom-up integrated platform of a high potential biofluid in regenerative medicine

Inserra, Ilaria; Martelli, Claudia; Cipollina, Mara; Cicione, Claudia; Iavarone, Federica; Taranto, Giuseppe Di; Barba, Marta; Castagnola, Massimo; Desiderio, Claudia; Lattanzi, Wanda

doi:10.1002/elps.201500504

Cited by 14 publications

(14 citation statements)

References 70 publications

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“…In our study, liposculpture of the area was performed after the excision, in order to obtain better skin redraping and to avoid irregularities. We believe that liposuction can play a key role in the final step of this procedure, with the goal of minimizing any irregularities [19,20]. After the resection, the surgeon can properly evaluate the stored area of fatty tissue and the presence of any unpleasant, irregular defects.…”

Section: Discussionmentioning

confidence: 99%

The round-the-clock technique for correction of gynecomastia

et al. 2019

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Background Gynecomastia is a common condition that can cause severe emotional and physical distress in both young and older men. Patients in whom symptomatic recalcitrant gynecomastia persists for a long time are potential candidates for surgery. Methods From January 2014 to January 2016, 15 patients underwent correction of gynecomastia through a single 3-mm incision at our institution. Only patients with true gynecomastia underwent surgery with this new technique. Through the small incision, sharp dissection was performed in a clockwise and counterclockwise direction describing two half-circles. Health-related quality of life and aesthetic outcomes were evaluated using a modified version of the Breast Evaluation Questionnaire (BEQ). Results The patients’ average age was 23.5 years (range, 18–28 years), and their average body mass index was 23.2 kg/m 2 (range, 19.2–25.3 kg/m 2 ). One case was unilateral and 14 cases were bilateral. The weight of glandular tissue resected from each breast ranged from 80 to 170 g. No excess skin was excised. Bleeding was minimal. The mean operating time was 25 minutes (range, 21–40 minutes). No complications were recorded. All lesions were histologically benign. The patients’ average score was 3.5 (on a 5-point Likert scale) in all domains of the BEQ for themselves and their partners. Conclusions In this study, we demonstrated the safety and reliability of a new technique that allows mastectomy through an imperceptible 3-mm incision. We obtained high patient satisfaction scores using our surgical technique, and patients reported considerable improvement in their social, physical, and psychological well-being after surgery.

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Section: Discussionmentioning

confidence: 99%

The round-the-clock technique for correction of gynecomastia

et al. 2019

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“…Together with them, a panel of four peptides with molecular weight of about 3 kDa and corresponding to specific sequences of both aand b-globin chains has also been identified. These peptides, whose biological function is still not clear, have also been characterized in lipoaspirate fluid (Inserra et al 2016) and craniopharyngioma adamantinomatous pediatric brain tumor intracystic fluid (Martelli et al 2014). These peptides have been previously isolated in lysates of human erythrocytes (Karelin et al 1995).…”

Section: Hemoglobin Cryptidesmentioning

confidence: 99%

Cryptides: latent peptides everywhere

Iavarone

Desiderio

Vitali

et al. 2018

Critical Reviews in Biochemistry and Molecular Biology

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Proteomic surveys with top-down platforms are today revealing thousands of naturally occurring fragments of bigger proteins. Some of them have not functional meaning because they derive from pathways responsible for protein degradation, but many have specific functions, often completely different from that one of the parent proteins. These peptides encrypted in the protein sequence are nowadays called cryptides. They are frequent in the animal and plant kingdoms and represent a new interesting -omic field of investigation. To point out how much widespread is their presence, we describe here the most studied cryptides from very common sources such as serum albumin, immunoglobulins, hemoglobin, and from saliva and milk proteins. Given its vastness, it is unfeasible to cover the topic exhaustively, therefore only several selected examples of cryptides from other sources are thereafter reported. Demanding is the development of new -omic platforms for the functional screening of new cryptides, which could provide suggestion for peptides and peptido-mimetics with variegate fields of application.

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“…We have previously demonstrated that this fluid is able to exert angiogenic and osteoinductive properties in vitro [55]. We have further investigated the proteome-peptidome composition of LAF through a top down/bottom up approach [68]. This study allowed identifying numerous bioactive proteins, peptides and paracrine factors, such as albumin and hemoglobin fragments (i.e.…”

Section: Osteoinductive Properties Of Ascsmentioning

confidence: 99%

“…The LAF also featuredS100A6, a member of the S100 Ca 2+ -binding protein family. S100A6 induces bone formation modulating the capability of cells to sense extracellular cations [68].The documented presence ASC-like cells and bioactive molecules in the LAF, along with its rapid and easy isolation, make this fluid attractive and suitable for regenerative medicine applications, specifically as a "minimally manipulated tissue" to be tested in vivofor a potentially wide range of applications.…”

Section: Osteoinductive Properties Of Ascsmentioning

confidence: 99%

Adipose-derived stem cell therapies for bone regeneration

Barba

Taranto

Lattanzi

2017

Expert Opinion on Biological Therapy

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Cell-based therapies exploit the heterogeneous and self-sufficient biological environment of stem cells to restore, maintain and improve tissue functions. Adipose-derived stem cells (ASCs) are, to this aim, promising cell types thanks to advantageous isolation procedures, growth kinetics, plasticity and trophic properties. Specifically, bone regeneration represents a suitable, though often challenging, target setting to test and apply ASC-based therapeutic strategies. Areas covered: ASCs are extremely plastic and secrete bioactive peptides that mediate paracrine functions, mediating their trophic actions in vivo. Numerous preclinical studies demonstrated that ASCs improve bone healing. Clinical trials are ongoing to validate the clinical feasibility of these approaches. This review is intended to define the state-of-the-art on ASCs, encompassing the biological features that make them suitable for bone regenerative strategies, and to provide an update on existing preclinical and clinical applications. Expert opinion: ASCs offer numerous advantages over other stem cells in terms of feasibility of clinical translation. Data obtained from in vivo experimentation are encouraging, and clinical trials are ongoing. More robust validations are thus expected to be achieved during the next few years, and will likely pave the way to optimized patient-tailored treatments for bone regeneration.

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Lipoaspirate fluid proteome: A preliminary investigation by LC-MS top-down/bottom-up integrated platform of a high potential biofluid in regenerative medicine

Cited by 14 publications

References 70 publications

The round-the-clock technique for correction of gynecomastia

The round-the-clock technique for correction of gynecomastia

Cryptides: latent peptides everywhere

Adipose-derived stem cell therapies for bone regeneration

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