Lipo Layering of Autologous Fat: An Improved Technique with Promising Results

Ersek, Robert A.; Chang, Peter; Salisbury, Mark

doi:10.1097/00006534-199803000-00038

Cited by 114 publications

(58 citation statements)

References 25 publications

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“…The mean value of VSF is high in relation to other rates published in the literature (Assadi and Haramis, 1993;Niechajevia and Sevcuk, 1994;Ersek et al, 1998). There is a remarkable difference between our 66% survival and the 90% survival reported by Coleman (1995;1997) and with the study of Guerrerosantos (2000) that employed the same surgical procedure.…”

Section: Discussioncontrasting

confidence: 42%

First Use of Stereology to Quantify the Survival of Fat Autografts

Cuellar¹,

Solís²

2011

Image Anal Stereol

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It is not usual to perform quantitative analyses on surgical materials. Rather, they are evaluated clinically, through qualitative methods, and if quantitation is done, it is on a 2-dimensional basis. In this study, the long-term survival of fat autografts (FAG) in 40 subjects with facial soft tissue defects is quantified. An adipose tissue preparation from the abdomen obtained through liposuction and centrifugation is injected subcutaneously. Approximately 14 months later, the treated area is biopsied. Extensive computer-based histological analyses were performed using the stereological method in order to directly obtain three parameters: volume fraction of adipocytes in the fat tissue (V V ), density (number per volume) of adipocytes in the fat tissue (N V ), and the mean cell volume of adipocytes (VA) in each tissue sample. A set of equations based on these three quantitative parameters is produced for evaluation of the volumetric survival fraction (VSF) of FAG. The presented data evidenced a 66% survival fraction at the 14-month follow-up. In routine practice, it would be sufficient to perform this volumetric analysis on the injected and biopsied fat samples to know what fraction of the FAG has survived. This is an objective method for quantifying FAG survival and will allow a standardized comparison between different research series and authors.

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Section: Discussioncontrasting

confidence: 42%

First Use of Stereology to Quantify the Survival of Fat Autografts

Cuellar¹,

Solís²

2011

Image Anal Stereol

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“…This led to a new wave of negative publications, modifications and even an attempt for reviving the idea of the en block transplantation (Ellenbongen, 1986). On the other hand many authors in Europe and USA reported big series of patients with successful application of autologous fat tissue harvested by liposuction and each one of them suggested a personal technique of processing and reinjection of the graft (Asken, 1987;Bircoll, 1987;Bircoll, Novack, 1987;Carraway, Mellow,1990;Chajhir et al, 1990;Ersek et al, 1998;Illouz, 1986;Fournier, 1996). One of them is S.R.…”

Section: Wwwintechopencommentioning

confidence: 99%

Autologous Fat Grafting - Factors of Influence on the Therapeutic Results

Khater¹,

Atanassova²

2012

Current Concepts in Plastic Surgery

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“…Adequate vascularization of the tissue engineered fat is still the most important problem in preventing more extensive use of this tissue. Free adipose tissue grafts are largely absorbed or replaced by fibrous tissue and oil cysts [7], even though some reports demonstrate increasing survival of the transplanted tissue by careful handling [8,9].…”

Section: Introductionmentioning

confidence: 99%

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

Hemmrich¹,

Sijpe²,

Rhodes³

et al. 2008

Journal of Surgical Research

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Background. There is a major clinical need for strategies for adequately reconstructing the soft tissue defects found after deep burns, tumor resection, or trauma. A promising solution is adipose tissue engineering with preadipocytes, stem-cell derived precursors of the adipose tissue, implanted within biomaterials. This pilot study evaluated hyaluronan gels mixed with autologous undifferentiated preadipocytes in a pig model for their potency to generate new fat.Materials and methods. Preadipocytes were isolated from intra-abdominal pig fat by collagenase digestion, plated on fibronectin-coated culture dishes in Dulbecco's Modified Eagle Medium/Ham's F12 (Biochrom, Berlin, Germany) combined with 10% pig serum, expanded, and mixed with hyaluronan gel. Two types of gels with varying degrees of amidation of the carboxyl groups were tested (HYADD3, HYADD4). Cell-loaded gels and unseeded controls were injected subcutaneously into the ears of three pigs, explanted at 6 wk, and analyzed histologically.Results. Both cell-loaded specimens were detected macroscopically. They demonstrated a slight volume effect with limited stability after 6 wk. Unloaded HYADD3 and HYADD4 controls could not be identified at the time of explantation. Histology of HYADD3 revealed islets of mature adipocytes and vessels embedded in fat tissue surrounded by gel. In contrast, no fat formation was found in HYADD4 gels when implanted in the ear.Conclusions. Histological findings demonstrate that HYADD3 is a promising gel for generating adipose tissue. Even though HYADD3 might be a potential material for the reconstruction of small tissue defects, the question remains as to whether the adipose tissue within the gel is attributable to preadipocyte maturation or ingrowth from neighboring tissue.

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Lipo Layering of Autologous Fat: An Improved Technique with Promising Results

Cited by 114 publications

References 25 publications

First Use of Stereology to Quantify the Survival of Fat Autografts

First Use of Stereology to Quantify the Survival of Fat Autografts

Autologous Fat Grafting - Factors of Influence on the Therapeutic Results

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

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