LipidTOX: A fatty acid-based index efficient for ecotoxicological studies with marine model diatoms exposed to legacy and emerging contaminants

Duarte, Bernardo; Feijão, Eduardo; Franzitta, Marco; Duarte, Irina A.; Carvalho, Ricardo Cruz de; Cabrita, Maria Teresa; Marques, João; Caçador, Isabel; Fonseca, Vanessa F.; Matos, Ana Rita

doi:10.1016/j.ecolind.2022.108885

Cited by 5 publications

(4 citation statements)

References 59 publications

(106 reference statements)

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“…Among these, metabolites identified as potential biomarkers comprised five lipid and fatty acid metabolites. This is in line with our previous findings where it was shown that some of the most relevant effects of fluoxetine exposure in P. tricornutum occurred at the lipid and fatty acid metabolism [ 10 ], with a proposed fatty acid-based integrative index developed for improved ecotoxicological assessments in fluoxetine and other emerging contaminants [ 23 ]. Another interesting biomarker identified by this untargeted FT-ICR-MS metabolomic approach was chlorophyll a precursor, chlorophyllide a [ 61 ].…”

Section: Discussionsupporting

confidence: 90%

“…In ecotoxicological terms and considering the diatom P. tricornutum as a model species for ecotoxicology, biomarker discovery approaches that can simultaneously provide insights on the mode of action of any potentially toxic compound and be a source of potential biomarkers, are key for future environmental risk assessments. In the past, several biomarkers were developed for this diatom species, focusing specifically on targeted metabolism [ 23 , 24 , 25 ], thus an untargeted approach would be of added value to provide a more comprehensive evaluation of the whole diatom metabolism. This metabolomic approach to ecotoxicological assays is very recent but promising for marine ecotoxicology [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…This is of great concern as diatoms usually account for a large proportion of the primary producers at the basis of marine trophic webs. Therefore, it is fundamental to understand the impacts of fluoxetine at the metabolic level in non-target organisms, such as the model diatom Phaeodactylum tricornutum , to also better understand the metabolic pathways governing the toxicological effects observed in prototrophic organisms [ 10 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Untargeted Metabolomics Reveals Antidepressant Effects in a Marine Photosynthetic Organism: The Diatom Phaeodactylum tricornutum as a Case Study

Duarte

Feijão

Carvalho

et al. 2022

Biology

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The increased use of antidepressants, along with their increased occurrence in aquatic environments, is of concern for marine organisms. Although these pharmaceutical compounds have been shown to negatively affect marine diatoms, their mode of action in these non-target, single-cell phototrophic organisms is yet unknown. Using a Fourier-transform ion cyclotron-resonance mass spectrometer (FT-ICR-MS) we evaluated the effects of fluoxetine in the metabolomics of the model diatom Phaeodactylum tricornutum, as well as the potential use of the identified metabolites as exposure biomarkers. Diatom growth was severely impaired after fluoxetine exposure, particularly in the highest dose tested, along with a down-regulation of photosynthetic and carbohydrate metabolisms. Notably, several mechanisms that are normally down-regulated by fluoxetine in mammal organisms were also down-regulated in diatoms (e.g., glycerolipid metabolism, phosphatidylinositol signalling pathway, vitamin metabolism, terpenoid backbone biosynthesis and serotonin remobilization metabolism). Additionally, the present work also identified a set of potential biomarkers of fluoxetine exposure that were up-regulated with increasing fluoxetine exposure concentration and are of high metabolic significance following the disclosed mode of action, reinforcing the use of metabolomics approaches in ecotoxicology.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Untargeted Metabolomics Reveals Antidepressant Effects in a Marine Photosynthetic Organism: The Diatom Phaeodactylum tricornutum as a Case Study

Duarte

Feijão

Carvalho

et al. 2022

Biology

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“…Contaminants like glyphosate, copper and some selected pharmaceutical products were evaluated by Duarte et al (2022). Diatom fatty acids in this study were affected by the applied xenobiotic type and concentrations, since saturated and monounsaturated C16 FAs showed a transversal increase to all exposures tested.…”

Section: Introductionmentioning

confidence: 94%

Mixed light photoperiod and biocide pollution affect lipid profiles of periphyton communities in freshwater ecosystems

Mazzella,

Vrba,

Moreira

et al. 2023

Preprint

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Environmental factors, such as light intensity and exposure to contaminants, may significantly influence the lipid composition of algae in periphytic communities. In this study, we investigated the joint effects of dodecylbenzyldimethylammonium chloride (BAC 12), as biocide, and light photoperiods on the polar lipidome of a freshwater biofilm. Exposure to BAC 12 in a microcosm experiment increased the heterotrophic compartment, while phototrophic organisms were severely affected, with corresponding shifts in lipid composition. The overall decrease in polyinsaturated fatty acids suggested a significant impact of the biocide on biofilm microalgae. However, it was difficult to distinguish the influence of light from that of contamination, as there was no observable effect of photoperiod on conventional fatty acid determination. Thus, the molecular species compositions of both glycolipids and phospholipids were explored in additional multivariate analyses. The results suggested that certain molecular species can serve as more specific markers of light duration at the biofilm scale, independently of the chemical pressure caused by other pollutants.

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Mixed light photoperiod and biocide pollution affect lipid profiles of periphyton communities in freshwater ecosystems

Mazzella,

Vrba,

Moreira

et al. 2023

Journal of Hazardous Materials Advances

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LipidTOX: A fatty acid-based index efficient for ecotoxicological studies with marine model diatoms exposed to legacy and emerging contaminants

Cited by 5 publications

References 59 publications

Untargeted Metabolomics Reveals Antidepressant Effects in a Marine Photosynthetic Organism: The Diatom Phaeodactylum tricornutum as a Case Study

Untargeted Metabolomics Reveals Antidepressant Effects in a Marine Photosynthetic Organism: The Diatom Phaeodactylum tricornutum as a Case Study

Mixed light photoperiod and biocide pollution affect lipid profiles of periphyton communities in freshwater ecosystems

Mixed light photoperiod and biocide pollution affect lipid profiles of periphyton communities in freshwater ecosystems

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