Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization

Abstract

Microalgae are one of the most promising sources of raw material for biofuel production and derivatives since its high yield of biomass and metabolites possess a low environmental impact. However, its implementation on large scale facilites still faces challenges such as the optimisation of lipid production (due to strain capacity and environmental factors) and downstream processes (extraction and separation of the lipidic fraction). The objective of the present investigation was to determine the potential of the carbon/nitrogen ratio as a technical tool for the improvement of total lipids on Scenedesmus obliquus. The carbon/nitrogen ratio was evaluated using a non-factorial design coupled with surface response methodology with sodium bicarbonate and sodium nitrate as carbon and nitrogen source. Results showed that the optimal conditions that enhanced the lipid deposition (up to 66% w/w) were 1.5 g L−1 sodium bicarbonate and 0,125 g L−1 of sodium nitrate. Finally, the results of the fatty acid profile shown the presence of stearic acid (C18: 0) with 22.63% and elaidic acid (C18:1) with 77.38%, with the absence of fatty acids of two or more double bonds. In conclusion, the adjustment in the carbon/nitrogen ratio favours the final deposition of lipids in Scenedesmus obliquus which is emerging as a possible candidate for the production of lipids of interest for the generation of biodiesel.

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

“…Over the years, this method has been proven to be an interesting statistical tool for better culture media in strains such as Ankistrodesmus falcatus KJ671624, 48 Auxenochlorella protothecoides , 49 Botryococcus braunii , 24 B. braunii LB572, 50 Chlorella sp., 51 Chlorellaprotothecoides , 52 Chlorella sorokiniana Mb-1, 53 Chlorella vulgaris , 54 C. vulgaris 1803, 55 Chlorococcum oleofaciens , 17 Desmodesmus armtus , 56 Dunaliella salina , 57 C. pyrenoidosa , 58 Scenedesmus sp. ASK22, 59 Scenedesmus obliquus , 60 , 61 and Spirulina sp. 62 In PBP research, the application of RSM has been focused on their extraction and purification.…”

First report of microalgae <em>Rhexinema paucicellulare</em> (Ulvophyceae) in Mauritius and its biochemical evaluation as a source of fatty acids

“…29 Strains of S. obliquus were found to produce 39.73% of lipids under normal culture conditions. 30 N. salina CCMP1176 yielded a lipid content of 36.95% ± 0.91 while N. granulata CCMP525 yielded a content of 60.35% ± 1.20. 31 However, it should be noted that light intensity has considerable influence on how microalgae can produce and/or increase lipids.…”

Evaluation of the Light/Dark Cycle and Concentration of Tannery Wastewater in the Production of Biomass and Metabolites of Industrial Interest from Microalgae and Cyanobacteria

“…Microalgae and cyanobacterium are renowned for their high photosynthetic efficiency and ability to generate different metabolites of interest, such as lipids [9], proteins [10], pigments [11], and carbohydrates [12]. Three key elements are needed to produce this microorganism: (1) a carbon source (usually in the form of CO 2 or inorganic form); (2) a culture medium with a sufficient nutrient concentration; and (3) a source of energy-in this case, light, which is known as photosynthetically active radiation [13,14].…”